| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
ABMDMA targets singlet oxygen (¹O2), a reactive oxygen species produced during photodynamic reactions and oxidative stress. It is not a biological receptor target but rather a chemical trap that reacts specifically with singlet oxygen.
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|---|---|
| ln Vitro |
ABMDMA undergoes photobleaching when exposed to singlet oxygen, transforming into its corresponding endoperoxide. This reaction causes a time-dependent decrease in absorbance at 380-400 nm, which can be monitored spectrophotometrically. The probe is insensitive to H2O2 or peroxides.
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| ln Vivo |
No in vivo activity data are documented as ABMDMA is a chemical probe for detecting singlet oxygen in biochemical and photochemical systems, not a therapeutic agent for systemic administration.
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| Enzyme Assay |
This assay is performed in a cuvette or microplate containing 10-100 uM ABMDMA in aqueous buffer (pH 7.4). Singlet oxygen is generated using a photosensitizer (e.g., Rose Bengal, methylene blue) with light irradiation, or chemically using thermolabile endoperoxide donors. Absorbance at 400 nm is measured at regular intervals to monitor the decrease due to endoperoxide formation.
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| Cell Assay |
ABMDMA is not typically used in live-cell assays due to potential cellular toxicity. Instead, cell lysates or cell culture media are collected, and 10-100 uM ABMDMA is added to the sample. Singlet oxygen generated by photodynamic treatment or by cells is quantified by measuring the decrease in absorbance at 400 nm over time.
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| Animal Protocol |
ABMDMA is not for in vivo animal administration. For ex vivo tissue studies, tissue homogenates are incubated with 50-100 uM ABMDMA, and singlet oxygen production is induced by light irradiation or photosensitizers. The decrease in absorbance at 400 nm is monitored spectrophotometrically to quantify ROS generation.
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| ADME/Pharmacokinetics |
ABMDMA is stable as a solid at room temperature, but stock solutions should be prepared fresh and protected from light. It shows good water solubility due to its dimalonic acid side chains. Solubility: soluble in water and DMSO. Spectrophotometric detection: measure absorbance decrease at 400 nm for singlet oxygen detection.
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| Toxicity/Toxicokinetics |
Toxicity data are limited as ABMDMA is a research-grade chemical probe not intended for in vivo use. When handling, standard laboratory safety protocols should be followed, including the use of gloves and eye protection. Avoid exposure to light to prevent premature photobleaching.
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| References | |
| Additional Infomation |
This is a research-use-only chemical probe, not an approved drug or dye. It is suitable for assessing the photodynamic effect of photosensitizers, including curcumin. Molecular formula: C22H18O8; molecular weight: 410.37. Also known as ABMDMA. The absorption, emission, and excitation spectra are not pH-dependent.
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| Molecular Formula |
C22H18O8
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|---|---|
| Molecular Weight |
410.37352
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| Exact Mass |
410.1
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| CAS # |
307554-62-7
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| PubChem CID |
3458978
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.527g/cm3
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| Boiling Point |
745.4ºC at 760 mmHg
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| Flash Point |
418.5ºC
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| Index of Refraction |
1.717
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| LogP |
2.648
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
30
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| Complexity |
579
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
DNUYOWCKBJFOGS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H18O8/c23-19(24)17(20(25)26)9-15-11-5-1-2-6-12(11)16(10-18(21(27)28)22(29)30)14-8-4-3-7-13(14)15/h1-8,17-18H,9-10H2,(H,23,24)(H,25,26)(H,27,28)(H,29,30)
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| Chemical Name |
2-[[10-(2,2-dicarboxyethyl)anthracen-9-yl]methyl]propanedioic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~243.68 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4368 mL | 12.1841 mL | 24.3683 mL | |
| 5 mM | 0.4874 mL | 2.4368 mL | 4.8737 mL | |
| 10 mM | 0.2437 mL | 1.2184 mL | 2.4368 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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