| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
8-Phenyl-BODIPY 505/515 does not have a specific biological target as it is a fluorescent dye. Its function is to provide fluorescence for imaging and detection applications. The compound is used as a fluorescent probe for various bioimaging applications. It is particularly used for labeling lipid droplets in cells and tissues, demonstrating good fluorescence performance and stability. The phenyl substitution alters the phosphorescent properties of BODIPY, affecting triplet and singlet oxygen generation.
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| ln Vitro |
In vitro, 8-Phenyl-BODIPY 505/515 is used as a fluorescent probe for various bioimaging applications. The compound is a phenyl-substituted BODIPY derivative and a fluorophore. It is particularly useful for labeling lipid droplets in cells and tissues, demonstrating good fluorescence performance and stability. The phenyl substitution alters the phosphorescent properties of BODIPY, affecting triplet and singlet oxygen generation. The compound's fluorescence properties make it suitable for fluorescence microscopy and other imaging applications.
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| ln Vivo |
8-Phenyl-BODIPY 505/515 is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a research tool for fluorescence-based imaging. The compound is used as a fluorescent probe for various bioimaging applications. It is not administered to animals for therapeutic purposes but is used in the preparation of solutions for biological imaging experiments.
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| Enzyme Assay |
In vitro experiments with 8-Phenyl-BODIPY 505/515 typically involve preparing stock solutions in DMSO or other organic solvents and diluting them in assay buffers or cell culture media. For cell staining, cells are incubated with the dye at concentrations of 1-10 µM for 15-60 minutes. After washing, cells are imaged using fluorescence microscopy with appropriate excitation and emission filters (typically excitation around 505 nm and emission around 515 nm). The dye is used for labeling lipid droplets and other cellular structures.
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| Cell Assay |
In vitro cell-based assays using 8-Phenyl-BODIPY 505/515 are performed to study cellular structures and processes using fluorescence imaging. Cells are incubated with the dye at concentrations of 1-10 µM for 15-60 minutes. After washing, cells are imaged using fluorescence microscopy. The dye is particularly useful for labeling lipid droplets in cells. The compound's fluorescence properties allow for the visualization of lipid droplets and other cellular structures. The dye is typically dissolved in DMSO and diluted in cell culture medium.
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| Animal Protocol |
Specific in vivo animal experiment protocols for 8-Phenyl-BODIPY 505/515 are not detailed in the available literature. The compound is primarily used as a fluorescent probe for in vitro applications. For potential in vivo imaging applications, the dye would need to be formulated for administration. The compound's fluorescence properties could allow for in vivo imaging, but such applications are not standard for this compound.
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| ADME/Pharmacokinetics |
8-Phenyl-BODIPY 505/515 has a molecular weight of 324.17 g/mol and the formula C19H19BF2N2. The compound is a phenyl-substituted BODIPY derivative and a fluorophore. For storage, the compound is kept at -20°C, protected from light. Its purity is typically ≥97%. The compound is used as a fluorescent probe for various bioimaging applications. It is particularly used for labeling lipid droplets in cells and tissues. The phenyl substitution alters the phosphorescent properties of BODIPY.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 8-Phenyl-BODIPY 505/515 are not provided in the available sources. As a fluorescent dye, it is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The dye may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References |
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| Additional Infomation |
4,4-Difluoro-1,3,5,7-Tetramethyl-8-phenyl-4-boron-3a,4a-diaza-s-indene is a BODIPY dye.
8-Phenyl-BODIPY 505/515 (CAS 194235-40-0) is a phenyl-substituted BODIPY derivative and a fluorophore. It has the molecular formula C19H19BF2N2 and a molecular weight of 324.17 g/mol. The phenyl substitution alters the phosphorescent properties of BODIPY, affecting triplet and singlet oxygen generation. 8-Phenyl-BODIPY 505/515 can be used as a fluorescent probe. It is used for various bioimaging applications, particularly for labeling lipid droplets in cells and tissues, demonstrating good fluorescence performance and stability. The compound is intended for research use only. |
| Molecular Formula |
C19H19BF2N2
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|---|---|
| Molecular Weight |
324.1754
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| Exact Mass |
324.16
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| CAS # |
194235-40-0
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| PubChem CID |
25058170
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| Appearance |
Orange to red solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
429.5±55.0 °C at 760 mmHg
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| Melting Point |
174-179°C
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| Flash Point |
213.5±31.5 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.544
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| LogP |
6.34
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
24
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| Complexity |
616
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
QFNRXPJMRSOECW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C19H19BF2N2/c1-12-10-14(3)23-18(12)17(16-8-6-5-7-9-16)19-13(2)11-15(4)24(19)20(23,21)22/h5-11H,1-4H3
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| Chemical Name |
2,2-difluoro-4,6,10,12-tetramethyl-8-phenyl-3-aza-1-azonia-2-boranuidatricyclo[7.3.0.03,7]dodeca-1(12),4,6,8,10-pentaene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0847 mL | 15.4235 mL | 30.8471 mL | |
| 5 mM | 0.6169 mL | 3.0847 mL | 6.1694 mL | |
| 10 mM | 0.3085 mL | 1.5424 mL | 3.0847 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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