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| Other Sizes |
| Targets |
8-Hydroxyquinoline hemisulfate targets bacterial cells and fungi through its ability to chelate metal ions, thereby disrupting essential metal-dependent enzymatic processes. It functions as a transcription inhibitor, interfering with gene expression in microorganisms. The compound's antimicrobial activity is attributed to its metal-chelating properties, which deprive microorganisms of essential metal cofactors required for growth and metabolism. It exhibits activity against a broad spectrum of microorganisms, including bacteria and fungi.
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| ln Vitro |
8-Hydroxyquinoline hemisulfate demonstrates mild bacteriostatic and fungistatic activity in vitro against a variety of microorganisms. It exhibits antiseptic, disinfectant, and pesticide properties. The compound's metal-chelating activity contributes to its antimicrobial effects by disrupting essential metal-dependent cellular processes. It is also used as a reagent in biochemical assays and as a preservative in various applications.
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| ln Vivo |
In vivo activity of 8-hydroxyquinoline hemisulfate has been documented in its use as an antiseptic, disinfectant, and pesticide. It has been employed as a radioactive indium carrier for diagnostic imaging purposes. The compound's anthelmintic and amebicidal actions suggest potential for treating parasitic infections. However, detailed in vivo efficacy data against specific pathogens are limited in the available literature.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for 8-hydroxyquinoline hemisulfate typically involve measuring its metal-chelating activity using spectrophotometric or fluorometric methods. The compound's ability to bind metal ions such as iron, copper, or zinc can be assessed by monitoring changes in absorbance or fluorescence upon complex formation. Its transcription inhibitory activity can be evaluated using reporter gene assays in bacterial or eukaryotic cells.
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| Cell Assay |
In vitro cellular assays for 8-hydroxyquinoline hemisulfate involve culturing bacterial or fungal cells in the presence of varying concentrations of the compound. Antimicrobial activity is assessed by measuring inhibition of growth using optical density measurements or colony counting. The compound's cytotoxic effects on mammalian cells can also be evaluated using standard cell viability assays such as MTT or CCK-8. These assays demonstrate the compound's broad-spectrum antimicrobial activity.
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| Animal Protocol |
In vivo animal experiments for 8-hydroxyquinoline hemisulfate are not extensively documented in the literature. The compound has been used as an antiseptic and disinfectant in various settings, but detailed animal study protocols are limited. Its use as a radioactive indium carrier for diagnostic purposes suggests potential for imaging applications in animal models. Further in vivo studies would be needed to characterize its efficacy and safety.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 8-hydroxyquinoline hemisulfate are limited in the available literature. As a metal-chelating agent, its absorption, distribution, metabolism, and excretion would be influenced by its ability to bind metal ions and form complexes. The compound's pharmacokinetic properties have not been extensively characterized in vivo. Further studies are needed to determine its systemic exposure and elimination pathways.
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| Toxicity/Toxicokinetics |
8-Hydroxyquinoline hemisulfate demonstrates a positive result for Drug Induced Liver Injury (DILI) according to the DILIps validation dataset, indicating potential hepatotoxic effects. The compound should be handled with appropriate safety precautions. It is intended for research use only and is not for human therapeutic application. Standard laboratory safety protocols should be followed when handling this compound.
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| Additional Infomation |
8-Hydroxyquinoline sulfate is a pale yellow to yellow crystalline powder with a slight saffron odor and a burning sensation. (NTP, 1992)
Hydroxyquinoline sulfate is a polymer. It is a preservative with mild antibacterial, bacteriostatic, anthelmintic, and amoebic effects. It is also used as a reagent and metal chelating agent, as well as a radioactive indium carrier for diagnostic purposes. Furthermore, its halogenated derivatives are used as topical anti-infective agents and oral anti-amoebic drugs. 8-Hydroxyquinoline hemisulfate (8-Quinolinol hemisulfate) (CAS#: 134-31-6) is a monoprotic bidentate chelating agent with antiseptic, disinfectant, and pesticide properties. It functions as a transcription inhibitor and has mild antimicrobial, anthelmintic, and amebicidal effects. The compound is used as a preservative, reagent, and metal chelating agent. It is intended for research use only and is not for human therapeutic application. |
| Molecular Formula |
C9H7NO.1/2H2O4S
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|---|---|
| Molecular Weight |
194.20
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| Exact Mass |
388.072
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| CAS # |
134-31-6
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| Related CAS # |
8-Hydroxyquinoline;148-24-3
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| PubChem CID |
517000
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.26g/cm3
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| Boiling Point |
267ºC at 760 mmHg
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| Melting Point |
~175 °C
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| Flash Point |
143.1ºC
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| Vapour Pressure |
8.34E-14mmHg at 25°C
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| LogP |
4.308
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
27
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| Complexity |
219
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C12=CC=CN=C1C(O)=CC=C2.C12=CC=CN=C1C(O)=CC=C2.S(=O)(=O)(O)O
|
| InChi Key |
YYVFXSYQSOZCOQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/2C9H7NO.H2O4S/c2*11-8-5-1-3-7-4-2-6-10-9(7)8;1-5(2,3)4/h2*1-6,11H;(H2,1,2,3,4)
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| Chemical Name |
quinolin-8-ol;sulfuric acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1493 mL | 25.7467 mL | 51.4933 mL | |
| 5 mM | 1.0299 mL | 5.1493 mL | 10.2987 mL | |
| 10 mM | 0.5149 mL | 2.5747 mL | 5.1493 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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