| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
7-Methoxycoumarin-3-carboxylic acid does not have a specific biological target. It functions as a fluorescent probe and derivatization agent. Its coumarin core provides fluorescence properties, making it useful for labeling and detecting biomolecules. It is used in FRET-based assays to study protease activities, where it serves as a donor or acceptor fluorophore.
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| ln Vitro |
7-Methoxycoumarin-3-carboxylic acid (compound 5) has an IC50 of >100, >100, >100, and > 100 μM, respectively, and is harmless to K562, Daudi, RPMI-8226, and Jurkat cells [1].
In vitro, 7-Methoxycoumarin-3-carboxylic acid is used as a fluorescent label in various biochemical assays. It is suitable for fluorescence-based applications and is used for HPLC derivatization to enhance the detection of analytes. Its role in FRET probes allows for the analysis of protease activities by monitoring changes in fluorescence upon cleavage of a peptide linker. |
| ln Vivo |
In vivo applications of 7-Methoxycoumarin-3-carboxylic acid are not typical, as it is primarily used in vitro as a fluorescent probe and derivatization reagent. It is not administered to living animals for therapeutic or diagnostic purposes. Its use is confined to laboratory research settings.
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| Enzyme Assay |
A cell-free assay for 7-Methoxycoumarin-3-carboxylic acid would involve its use as a fluorescent probe in biochemical assays. For example, it can be used in FRET-based protease assays where a peptide substrate labeled with the coumarin and a quencher is cleaved by the protease, resulting in a change in fluorescence. The compound's fluorescence properties can be characterized spectroscopically.
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| Cell Assay |
Cellular experiments with 7-Methoxycoumarin-3-carboxylic acid are not typical, as it is primarily used as a biochemical reagent rather than a cell-based probe. Its use is confined to in vitro biochemical assays and HPLC applications.
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| Animal Protocol |
In vivo animal experiments for 7-Methoxycoumarin-3-carboxylic acid are not applicable, as it is a research reagent used in vitro.
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| ADME/Pharmacokinetics |
7-Methoxycoumarin-3-carboxylic acid is a small molecule with a molecular weight of 220.18. Its pharmacokinetic properties are not relevant as it is not a therapeutic drug. The compound is used in vitro for research applications. It is soluble in DMF, DMSO, and acetonitrile.
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| Toxicity/Toxicokinetics |
Toxicity data for 7-Methoxycoumarin-3-carboxylic acid are not provided in the available sources. As a fluorescent probe, it is generally considered to have low toxicity for its intended research applications. However, specific toxicological information is not available. The compound is intended for research use only and not for human consumption.
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| References |
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| Additional Infomation |
7-Methoxycoumarin-3-carboxylic acid (CAS: 20300-59-8) is a coumarin derivative used as a fluorescent probe. It is used for HPLC derivatization and for developing FRET probes to analyze protease activities. Its molecular formula is C11H8O5 and its molecular weight is 220.18. It appears as a white to almost white powder or crystal with a purity of ≥97.0% (HPCE). It is not a therapeutic drug and is strictly for research purposes.
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| Molecular Formula |
C11H8O5
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|---|---|
| Molecular Weight |
220.1782
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| Exact Mass |
220.037
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| CAS # |
20300-59-8
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| PubChem CID |
583941
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
424.4±45.0 °C at 760 mmHg
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| Melting Point |
194ºC
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| Flash Point |
172.4±22.2 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.611
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| LogP |
1.56
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
16
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| Complexity |
346
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VEEGNDSSWAOLFN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H8O5/c1-15-7-3-2-6-4-8(10(12)13)11(14)16-9(6)5-7/h2-5H,1H3,(H,12,13)
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| Chemical Name |
7-methoxy-2-oxochromene-3-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5417 mL | 22.7087 mL | 45.4174 mL | |
| 5 mM | 0.9083 mL | 4.5417 mL | 9.0835 mL | |
| 10 mM | 0.4542 mL | 2.2709 mL | 4.5417 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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