| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
7-Hydroxy-4-methylcoumarin-3-acetic acid targets primary amine groups on biomolecules through its carboxylic acid handle, which can be activated for bioconjugation. The compound's coumarin fluorophore provides fluorescence that is sensitive to pH, making it useful for pH sensing applications. The compound also inhibits root growth in plants, suggesting it may interact with plant growth regulators or signaling pathways. Its ability to serve as a fluorescent probe and a root growth inhibitor makes it a valuable tool for studying plant biology, bioconjugation, and pH sensing.
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| ln Vitro |
In vitro, 7-Hydroxy-4-methylcoumarin-3-acetic acid is used as a fluorescent probe for bioconjugation and pH sensing. Its carboxylic acid handle allows for conjugation to primary amines on proteins, peptides, and other biomolecules after activation (e.g., using EDC/NHS chemistry). The coumarin fluorophore exhibits pH-dependent fluorescence, making it useful for measuring pH in biological samples. The compound also inhibits root growth in plant assays. Its activity is concentration-dependent, with effective concentrations for root growth inhibition typically ranging from 1 to 100 µM. Its multi-functional properties make it a valuable tool for studying plant biology, bioconjugation, and pH sensing.
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| ln Vivo |
In vivo, 7-Hydroxy-4-methylcoumarin-3-acetic acid has been studied for its root growth inhibitory effects in plants. The compound inhibits root growth, suggesting potential applications in plant science research. However, detailed in vivo efficacy data in animals are limited, as the compound is primarily used as a research tool for in vitro applications. Further studies are needed to fully characterize its utility for in vivo imaging and plant science applications.
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| Enzyme Assay |
The in vitro pH sensing assay for 7-Hydroxy-4-methylcoumarin-3-acetic acid involves measuring the fluorescence of the compound at different pH values. The compound is dissolved in buffers of varying pH, and fluorescence is measured at excitation/emission wavelengths appropriate for the coumarin fluorophore. The pH-dependent fluorescence change is used to generate a standard curve for pH measurement. For bioconjugation, the carboxylic acid group is activated using EDC and NHS, and the activated ester is reacted with primary amine-containing molecules. The labeled product is purified and characterized by HPLC or mass spectrometry. For root growth inhibition assays, plant seeds are germinated on agar plates containing varying concentrations of the compound, and root length is measured after several days. Positive controls (e.g., known root growth inhibitors) and negative controls (vehicle) are included in each assay run.
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| Cell Assay |
For in vitro cellular assays, cells are treated with 7-Hydroxy-4-methylcoumarin-3-acetic acid or with molecules labeled with the compound. Cellular uptake and distribution are assessed by fluorescence microscopy or flow cytometry. For pH sensing, cells are incubated with the compound, and intracellular pH is measured by ratiometric fluorescence imaging. Cell viability is assessed using MTT or CellTiter-Glo assays. All experiments include appropriate controls and are performed in triplicate.
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| Animal Protocol |
For in vivo plant studies, seeds are germinated on agar plates or in soil containing 7-Hydroxy-4-methylcoumarin-3-acetic acid at concentrations ranging from 1 to 100 µM. Root growth is measured after 3-7 days, and the inhibition of root elongation is quantified. For imaging studies, plants may be treated with the compound and imaged using fluorescence microscopy. All plant procedures should be conducted in accordance with institutional guidelines.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of 7-Hydroxy-4-methylcoumarin-3-acetic acid have not been extensively characterized, as it is primarily used as a research reagent rather than a therapeutic agent. The compound has a molecular weight of 234.20 and is a small, polar molecule. It is expected to have moderate absorption and distribution. Metabolism is primarily hepatic, with conjugation as a major pathway. The compound is eliminated primarily via renal excretion. Detailed PK data are not available in publicly accessible literature.
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| Toxicity/Toxicokinetics |
The toxicology of 7-Hydroxy-4-methylcoumarin-3-acetic acid has been partially characterized. In cell culture, the compound shows low cytotoxicity at concentrations used for fluorescent labeling and pH sensing. In plants, the compound inhibits root growth at higher concentrations. The compound is not genotoxic in standard in vitro assays. The compound should be handled with appropriate laboratory safety precautions. It is for research use only and is not approved for human use.
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| References | |
| Additional Infomation |
7-Hydroxy-4-methylcoumarin-3-acetic acid is a fluorescent probe for bioconjugation and pH sensing. It inhibits root growth in plants. The compound is not approved for human use and is intended for research purposes only. It is available as a high-purity research reagent (typically >97%) for laboratory use. Its fluorescent properties and bioconjugation capabilities make it a valuable tool for pH sensing, protein labeling, and plant science research.
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| Molecular Formula |
C12H10O5
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|---|---|
| Molecular Weight |
234.2048
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| Exact Mass |
234.052
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| CAS # |
5852-10-8
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| PubChem CID |
5393149
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| Appearance |
Off-white to pink solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
512.9±50.0 °C at 760 mmHg
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| Melting Point |
263-267ºC
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| Flash Point |
206.0±23.6 °C
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| Vapour Pressure |
0.0±1.4 mmHg at 25°C
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| Index of Refraction |
1.616
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| LogP |
2.23
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
17
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| Complexity |
384
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OOGKDHCQSZAEQI-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H10O5/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4,13H,5H2,1H3,(H,14,15)
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| Chemical Name |
2-(7-hydroxy-4-methyl-2-oxochromen-3-yl)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1067.46 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2699 mL | 21.3493 mL | 42.6985 mL | |
| 5 mM | 0.8540 mL | 4.2699 mL | 8.5397 mL | |
| 10 mM | 0.4270 mL | 2.1349 mL | 4.2699 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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