| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt does not have a biological target in the traditional pharmacological sense. Its mechanism of action is chemical, not biological. It acts as a fluorogenic probe by reacting specifically with thiol groups (-SH) on molecules. The reaction replaces the fluorine atom with the thiol group, forming a highly fluorescent adduct that can be detected and quantified.
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| ln Vitro |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt's "activity" is its ability to react with and fluorescently label thiols. In solution, it is non-fluorescent or weakly fluorescent, but upon reaction with thiols, it forms a highly fluorescent product. This property is used to detect and quantify thiols in various samples. It is not a pharmacological agent and does not exert biological effects like enzyme inhibition or receptor modulation.
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| ln Vivo |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt does not have in vivo pharmacological activity. It is a chemical reagent used for analytical purposes. It is not administered to animals for therapeutic or pharmacological studies. Its use is limited to in vitro and ex vivo applications for labeling thiols in biological samples.
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| Enzyme Assay |
Non-cellular assays for 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt involve its use as a fluorescent probe. In a typical protocol, a sample containing thiols is incubated with the reagent. The reaction is allowed to proceed, typically at a specific pH and temperature. The resulting fluorescence is measured using a spectrofluorometer or a fluorescence microplate reader. The fluorescence intensity is proportional to the concentration of thiols in the sample.
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| Cell Assay |
In vitro cellular assays for 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt are used to measure intracellular thiol levels. Cells are lysed, and the resulting cell lysate is treated with the probe. The fluorescent adduct is then analyzed, often by HPLC with fluorescence detection, to quantify the concentration of specific thiols like cysteine and glutathione. It is also used for labeling thiol-containing proteins in cell extracts.
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| Animal Protocol |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt is not used in in vivo animal studies as a therapeutic or pharmacological agent. Its application is limited to ex vivo analysis of biological samples collected from animals, such as blood, plasma, or tissues, to measure their thiol content.
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| ADME/Pharmacokinetics |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt is not a drug and does not have pharmacokinetic properties. It is a chemical reagent used for in vitro or ex vivo labeling. Its physical properties are more relevant; it is water-soluble, and its molecular weight is 235.20.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 7-Fluorobenzofurazan-4-sulfonic acid ammonium salt are not extensively detailed in standard product information. As a research chemical, it should be handled with standard laboratory precautions, avoiding inhalation, ingestion, and skin contact. It is not intended for human use.
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| References | |
| Additional Infomation |
7-Fluorobenzofurazan-4-sulfonic acid ammonium salt is a research-grade chemical reagent for laboratory use only and is not approved for clinical use. Its CAS number is 84806-27-9, and its molecular formula is C₆H₆FN₃O₄S. Its primary application is as a fluorescent labeling reagent for the detection and quantification of thiols, such as Cys and GSH, in biological samples using HPLC and fluorescence assays.
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| Molecular Formula |
C6H2N2O4FS-.H4N+
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|---|---|
| Molecular Weight |
235.19294
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| Exact Mass |
235.006
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| CAS # |
84806-27-9
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| PubChem CID |
121825
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.762 g/cm3
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| Boiling Point |
400.9ºC
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| Melting Point |
>290°C dec.
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| Flash Point |
196.3ºC
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| LogP |
2.013
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
15
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| Complexity |
316
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JXLHNMVSKXFWAO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3
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| Chemical Name |
azanium;7-fluoro-2,1,3-benzoxadiazole-4-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~250 mg/mL (~1062.97 mM)
H2O : ≥ 50 mg/mL (~212.59 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.2519 mL | 21.2594 mL | 42.5188 mL | |
| 5 mM | 0.8504 mL | 4.2519 mL | 8.5038 mL | |
| 10 mM | 0.4252 mL | 2.1259 mL | 4.2519 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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