| Size | Price | Stock | Qty |
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| 5g |
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| Other Sizes |
| Targets |
7-Ethoxy-4-methylcoumarin targets mammalian cytochromes P450, specifically isoforms 1A1, 2B4, and 2B6. As a substrate probe, it is metabolized by these enzymes to release the fluorescent product 7-hydroxy-4-methylcoumarin. The increase in fluorescence is proportional to enzyme activity. This makes the compound a valuable tool for studying cytochrome P450 enzyme activity, inhibition, and induction. The compound is used in drug metabolism studies and pharmaceutical research.
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| ln Vitro |
In vitro, 7-Ethoxy-4-methylcoumarin is used as a fluorogenic substrate in enzyme assays to measure cytochrome P450 activity. The compound is metabolized by P450 enzymes to release the fluorescent product 7-hydroxy-4-methylcoumarin. The increase in fluorescence is proportional to enzyme activity. The substrate is used in drug metabolism studies, enzyme kinetics, and high-throughput screening of P450 inhibitors. Its distinct chromophore makes it highly fluorescent upon enzymatic cleavage.
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| ln Vivo |
7-Ethoxy-4-methylcoumarin is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a research tool for in vitro enzyme assays and drug metabolism studies. The compound is used in pharmaceutical research to study cytochrome P450 enzyme activity. It is not administered to animals for therapeutic purposes but is used in the preparation of solutions for biological experiments.
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| Enzyme Assay |
In vitro enzyme assays for 7-Ethoxy-4-methylcoumarin typically involve incubating the substrate with cytochrome P450 enzymes (e.g., microsomal fractions) in an appropriate buffer containing NADPH as a cofactor. The compound is dissolved in DMSO or methanol and diluted in assay buffer to working concentrations (typically 1-100 µM). The reaction is incubated at 37°C for 10-30 minutes. The reaction is stopped, and the fluorescence of the product (7-hydroxy-4-methylcoumarin) is measured using a fluorescence plate reader with excitation at approximately 360-380 nm and emission at approximately 440-460 nm. The increase in fluorescence is proportional to enzyme activity.
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| Cell Assay |
In vitro cell-based assays using 7-Ethoxy-4-methylcoumarin are performed to measure cytochrome P450 activity in cultured cells. Cells are incubated with the substrate (typically 1-100 µM) for 1-4 hours. The fluorescent product 7-hydroxy-4-methylcoumarin is released into the culture medium. The medium is collected, and fluorescence is measured using a fluorescence plate reader with excitation at approximately 360-380 nm and emission at approximately 440-460 nm. The assay can be used to study P450 enzyme induction or inhibition in cells. The compound is typically dissolved in DMSO and diluted in cell culture medium.
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| Animal Protocol |
Specific in vivo animal experiment protocols for 7-Ethoxy-4-methylcoumarin are not detailed in the available literature. The compound is primarily used as a research tool for in vitro enzyme assays. For potential in vivo studies, the compound could be administered to animals to study P450 enzyme activity in vivo, but such applications are not standard. The compound's use in vivo would require formulation for administration and validation of the fluorescence signal in biological samples.
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| ADME/Pharmacokinetics |
7-Ethoxy-4-methylcoumarin has a molecular weight of 204.22 g/mol and the formula C12H12O3. The compound is a coumarin derivative and is also known as ethyl 4-methylumbelliferyl ether. It appears as a white solid. For storage, the compound is kept at room temperature in a dry place. Its purity is typically 97-99%. The compound is soluble in organic solvents such as DMSO and methanol. It is used as a substrate probe of mammalian cytochromes P450 1A1, 2B4, and 2B6.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 7-Ethoxy-4-methylcoumarin are not provided in the available sources. As a research chemical, it is intended for laboratory use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The compound may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References |
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| Additional Infomation |
7-Ethoxy-4-methyl-2H-1-benzopyran-2-one is a coumarin compound.
7-Ethoxy-4-methylcoumarin (CAS 87-05-8) is a coumarin derivative used as a substrate probe of mammalian cytochromes P450 1A1, 2B4, and 2B6. It has the molecular formula C12H12O3 and a molecular weight of 204.22 g/mol. The compound is also known as ethyl 4-methylumbelliferyl ether. 7-Ethoxy-4-methylcoumarin is a fluorescent probe that becomes highly fluorescent upon enzymatic cleavage. It is widely used in fluorogenic enzyme assays, pharmaceutical research, and biochemical studies. The compound is intended for research use only. |
| Molecular Formula |
C12H12O3
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|---|---|
| Molecular Weight |
204.2219
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| Exact Mass |
204.078
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| CAS # |
87-05-8
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| PubChem CID |
66595
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
351.4±37.0 °C at 760 mmHg
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| Melting Point |
114-116 °C(lit.)
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| Flash Point |
146.2±21.1 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.548
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| LogP |
2.9
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
283
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
NKRISXMDKXBVRJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H12O3/c1-3-14-9-4-5-10-8(2)6-12(13)15-11(10)7-9/h4-7H,3H2,1-2H3
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| Chemical Name |
7-ethoxy-4-methylchromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8967 mL | 24.4834 mL | 48.9668 mL | |
| 5 mM | 0.9793 mL | 4.8967 mL | 9.7934 mL | |
| 10 mM | 0.4897 mL | 2.4483 mL | 4.8967 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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