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| Targets |
7-Diethylamino-4-methylcoumarin does not have a specific biological target as it is a fluorescent dye. Its function is to provide bright blue fluorescence for various imaging and sensing applications. The compound's strong fluorescence properties make it useful as a fluorescent marker in biological assays. It is also used as a fluorescence probe for the investigation of hydrogen bond interactions. The compound's fluorescence can be excited by laser excitation and emits at red-shifted wavelengths.
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| ln Vitro |
In vitro, 7-Diethylamino-4-methylcoumarin is used as a fluorescent dye for various applications. It provides bright blue emission for sensor and imaging probe design. The compound is used as a fluorescent marker in biological assays. It is also used as a fluorescence probe for the investigation of hydrogen bond interactions. The compound's strong fluorescence properties make it suitable for fluorescence microscopy and photonics applications. It is also used as a laser dye.
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| ln Vivo |
7-Diethylamino-4-methylcoumarin is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a research tool for fluorescence-based assays and imaging. The compound is used in fluorescence microscopy, photonics, and as a fluorescent marker in biological assays. It is not administered to animals for therapeutic purposes but is used in the preparation of solutions for biological experiments.
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| Enzyme Assay |
In vitro experiments with 7-Diethylamino-4-methylcoumarin typically involve preparing stock solutions in DMSO or ethanol and diluting them in assay buffers. The dye exhibits bright blue fluorescence, and its fluorescence properties can be measured using a fluorescence spectrophotometer or plate reader with appropriate excitation and emission wavelengths (typically excitation around 370-390 nm and emission around 440-460 nm). The dye is used in fluorescence microscopy and flow cytometry applications. The compound is typically stored at room temperature, protected from light.
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| Cell Assay |
In vitro cell-based assays using 7-Diethylamino-4-methylcoumarin are performed to study cellular processes using fluorescence imaging. Cells are incubated with the dye at concentrations of 1-10 µM for 15-60 minutes. After washing, cells are imaged using fluorescence microscopy with appropriate excitation and emission filters. The dye's bright blue fluorescence allows for the visualization of cellular structures or the tracking of labeled molecules. The compound can also be used in flow cytometry applications. The dye is typically dissolved in DMSO and diluted in cell culture medium.
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| Animal Protocol |
Specific in vivo animal experiment protocols for 7-Diethylamino-4-methylcoumarin are not detailed in the available literature. The compound is primarily used as a fluorescent dye for in vitro applications. For potential in vivo imaging applications, the dye would need to be conjugated to targeting molecules and formulated for administration. The compound's fluorescence properties could allow for in vivo imaging, but such applications are not standard for this compound.
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| ADME/Pharmacokinetics |
7-Diethylamino-4-methylcoumarin has a molecular weight of 231.29 g/mol and the formula C14H17NO2. The compound appears as a white to amber to dark green powder to crystal with a melting point of 70-74°C. For storage, the compound is kept at room temperature, protected from light. Its purity is typically ≥98%. The compound is also known as Coumarin 1 and is potentially useful as a laser dye. It is used as a fluorescence probe for the investigation of hydrogen bond interactions.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 7-Diethylamino-4-methylcoumarin are not provided in the available sources. As a fluorescent dye, it is intended for research use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The dye may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References | |
| Additional Infomation |
7-Diethylamino-4-methylcoumarin is a light brown granular substance that emits bright blue-white fluorescence in very dilute solutions. (NTP, 1992)
Coumarin 460 is one of the 7-aminocoumarin compounds and functions as a fluorescent dye. 7-Diethylamino-4-methylcoumarin (CAS 91-44-1) is a classic fluorescent dye providing bright blue emission for sensor and imaging probe design. It is also known as Coumarin 1 and has the molecular formula C14H17NO2. The compound is a high-purity fluorescent dye with strong fluorescence properties, making it an ideal candidate for various applications in fluorescence microscopy, photonics, and as a fluorescent marker in biological assays. Coumarin 1 is potentially useful as a laser dye. It is used as a fluorescence probe for the investigation of hydrogen bond interactions. The compound is intended for research use only. |
| Molecular Formula |
C14H17NO2
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| Molecular Weight |
231.29028
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| Exact Mass |
231.125
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| CAS # |
91-44-1
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| PubChem CID |
7050
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| Appearance |
White to off-white solid powder
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
391.7±37.0 °C at 760 mmHg
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| Melting Point |
72-75 °C
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| Flash Point |
152.0±17.4 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.574
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| LogP |
4.15
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
17
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| Complexity |
320
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
AFYCEAFSNDLKSX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H17NO2/c1-4-15(5-2)11-6-7-12-10(3)8-14(16)17-13(12)9-11/h6-9H,4-5H2,1-3H3
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| Chemical Name |
7-(diethylamino)-4-methylchromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3236 mL | 21.6179 mL | 43.2358 mL | |
| 5 mM | 0.8647 mL | 4.3236 mL | 8.6472 mL | |
| 10 mM | 0.4324 mL | 2.1618 mL | 4.3236 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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