| Size | Price | Stock | Qty |
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| 5mg |
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| Targets |
6-ROX, SE does not have a specific biological target as a drug. Its primary application is as a fluorescent label for oligonucleotides, peptides, proteins, and other amine-containing biomolecules. The succinimidyl ester (SE) moiety reacts specifically with primary amines (-NH₂) to form stable amide bonds. This covalent labeling allows the dye to be attached to target molecules, enabling their detection and tracking in various assays, including automated DNA sequencing, capillary electrophoresis, and fluorescence-based detection methods.
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| ln Vitro |
In vitro, 6-ROX, SE is widely used for oligonucleotide labeling and automated DNA sequencing. It is a highly fluorescent, photostable rhodamine dye for various applications, including labeling of amino-groups in peptides, proteins, and amino-oligonucleotides. Compared to other rhodamines, ROX is noted to be very unstable, but the purified single isomer form is used for specific applications. Its fluorescence intensity and stability make it a preferred choice for sensitive detection methods.
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| ln Vivo |
In vivo activity of 6-ROX, SE is not typically studied, as it is a fluorescent labeling reagent rather than a bioactive drug. However, labeled biomolecules (e.g., labeled antibodies or oligonucleotides) can be used in vivo for imaging or tracking purposes. The dye's fluorescence can be detected in tissues, allowing researchers to study the distribution and dynamics of labeled molecules in living organisms. Its stability and fluorescence properties make it suitable for such applications, provided the labeled molecule is appropriately formulated.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays using 6-ROX, SE typically involve the dye as a label for biomolecules, rather than as a binding agent itself. A standard protocol for labeling oligonucleotides: the oligonucleotide is synthesized with a primary amine group at the 5' or 3' end. The amine-modified oligonucleotide is dissolved in a buffer (e.g., 0.1 M sodium bicarbonate, pH 8.5) and mixed with a solution of 6-ROX, SE in DMSO. The reaction mixture is incubated at room temperature for 1-2 hours in the dark. The labeled oligonucleotide is purified by ethanol precipitation or HPLC. The degree of labeling is determined by measuring the absorbance at 260 nm (oligonucleotide) and 585 nm (ROX).
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| Cell Assay |
In vitro cell-based assays for 6-ROX, SE typically involve using the dye to label cell surface proteins or to detect specific nucleic acid sequences. A standard protocol for labeling cell surface proteins: cells are harvested and incubated with a 6-ROX, SE-labeled antibody in PBS for 30-60 minutes at 4°C. Cells are washed to remove unbound antibody and analyzed by flow cytometry. The fluorescence intensity measured corresponds to the expression level of the target protein on the cell surface. Alternatively, 6-ROX-labeled oligonucleotide probes can be used in fluorescence in situ hybridization (FISH) to detect specific DNA or RNA sequences within cells.
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| Animal Protocol |
In vivo animal experiments for 6-ROX, SE are typically not performed for the dye itself, but for 6-ROX-labeled biomolecules. A standard protocol for biodistribution of a labeled antibody: a 6-ROX-labeled antibody is administered intravenously to mice. At various time points, animals are euthanized, and organs are collected. The tissues are homogenized, and the fluorescence intensity is measured using a fluorometer to determine the distribution of the antibody. Alternatively, whole-body imaging can be performed using an imaging system equipped with appropriate excitation and emission filters for ROX to visualize the localization of the labeled antibody in real-time.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 6-ROX, SE are not a primary focus of study. The compound is a fluorescent dye with a large molecular weight (631.67 g/mol) and is not expected to cross biological membranes readily. Once conjugated to a biomolecule, the pharmacokinetics of the conjugate are determined by the biomolecule rather than the dye. The dye itself is stable and photostable, making it suitable for long-term imaging and detection applications. It should be stored protected from light to prevent photobleaching.
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| Toxicity/Toxicokinetics |
Toxicity data for 6-ROX, SE is limited. As a fluorescent dye, it is generally considered to have low toxicity at the concentrations used for labeling and detection. However, as with all chemical reagents, appropriate safety precautions should be taken when handling the compound. It should be stored protected from light and handled with standard laboratory safety equipment. The compound is not intended for human use.
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| References | |
| Additional Infomation |
6-ROX, SE is an amine-reactive form of carboxy-X-rhodamine widely used for oligonucleotide labeling and automated DNA sequencing applications. It is a pure single isomer. The succinimidyl ester moiety enables covalent conjugation to amine-containing substrates. The dye exhibits high fluorescence intensity and stability. It is not a drug and has no clinical use or approval status. It is commercially available from chemical suppliers for research purposes only.
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| Molecular Formula |
C37H33N3O7
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|---|---|
| Molecular Weight |
631.67400
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| Exact Mass |
631.232
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| CAS # |
216699-36-4
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| PubChem CID |
2762610
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| Appearance |
Light brown to brown solid powder
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| LogP |
4.583
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
47
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| Complexity |
1490
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KWADUASJUMATIK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(36(43)44)26(19-22)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2
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| Chemical Name |
4-(2,5-dioxopyrrolidin-1-yl)oxycarbonyl-2-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5831 mL | 7.9155 mL | 15.8311 mL | |
| 5 mM | 0.3166 mL | 1.5831 mL | 3.1662 mL | |
| 10 mM | 0.1583 mL | 0.7916 mL | 1.5831 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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