| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
6-Amino-5-nitropyridin-2-one functions as an artificial nucleobase in hachimoji DNA, an eight-letter genetic alphabet. It specifically pairs with 5-aza-7-deazaguanine through hydrogen bonding, forming a non-natural base pair. This pairing expands the genetic code beyond the natural A-T and G-C base pairs, enabling the creation of DNA molecules with increased information storage capacity. The compound's primary "target" is its complementary synthetic nucleobase, 5-aza-7-deazaguanine, within the context of hachimoji DNA.
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| ln Vitro |
In vitro, 6-Amino-5-nitropyridin-2-one functions as a nucleobase that pairs with 5-aza-7-deazaguanine in hachimoji DNA. Its pairing properties have been characterized in biochemical studies of hachimoji DNA, demonstrating its ability to form stable base pairs with its complementary nucleobase. The compound is used in the synthesis of oligonucleotides containing non-natural base pairs for various applications in synthetic biology and DNA nanotechnology.
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| ln Vivo |
In vivo studies of 6-Amino-5-nitropyridin-2-one are not typically performed, as it is primarily a research tool for in vitro applications in synthetic biology. As an artificial nucleobase, its use is generally confined to cell-free systems or in vitro DNA synthesis. Future studies might explore its incorporation into living systems, but such applications are currently in the realm of basic research.
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| Enzyme Assay |
For in vitro enzyme/receptor binding assays, 6-Amino-5-nitropyridin-2-one is not typically used as an enzyme inhibitor or receptor ligand. Instead, it is used as a substrate or building block in DNA synthesis reactions. Its incorporation into DNA by polymerases can be studied using in vitro DNA synthesis assays. Its pairing specificity with 5-aza-7-deazaguanine can be assessed using thermal melting experiments or other biophysical methods to measure duplex stability.
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| Cell Assay |
For in vitro cellular experiments, 6-Amino-5-nitropyridin-2-one is not typically used in cell-based assays, as it is a synthetic nucleobase for DNA nanotechnology and synthetic biology. However, its incorporation into DNA constructs used in cell-based studies could be explored. The compound's effects on cell viability and function are not typically assessed, as it is not intended for therapeutic or cellular applications.
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| Animal Protocol |
For in vivo animal experiments, 6-Amino-5-nitropyridin-2-one is not typically administered to animals, as it is a research tool for in vitro applications. Its use is confined to synthetic biology and DNA nanotechnology research. Future studies might explore its potential for in vivo applications, but such studies are not currently reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 6-Amino-5-nitropyridin-2-one are not relevant, as it is used as a chemical building block rather than a therapeutic agent. The compound is not administered to animals or humans for therapeutic purposes. Its stability and solubility are important for its use in chemical synthesis and DNA nanotechnology applications.
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| Toxicity/Toxicokinetics |
Toxicological data for 6-Amino-5-nitropyridin-2-one are limited, as it is used as a chemical building block rather than a therapeutic agent. The compound is generally considered to have low toxicity for in vitro use. However, as with all research chemicals, appropriate safety precautions should be taken when handling the compound, including the use of personal protective equipment and adherence to institutional safety guidelines.
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| References | |
| Additional Infomation |
6-Amino-5-nitropyridin-2-one is a research compound used in synthetic biology and DNA nanotechnology. No clinical trials or regulatory approvals have been reported for this compound as a therapeutic agent. It is available from various chemical suppliers for research purposes only. The compound serves as a nucleobase in hachimoji DNA, pairing with 5-aza-7-deazaguanine.
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| Molecular Formula |
C5H5N3O3
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|---|---|
| Molecular Weight |
155.1115
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| Exact Mass |
155.033
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| CAS # |
211555-30-5
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| PubChem CID |
11182729
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.54g/cm3
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| Boiling Point |
305.9ºC at 760 mmHg
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| Flash Point |
138.8ºC
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| Vapour Pressure |
0.000799mmHg at 25°C
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| Index of Refraction |
1.634
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| LogP |
0.969
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
11
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| Complexity |
273
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JFGLOZOVAGYLKU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C5H5N3O3/c6-5-3(8(10)11)1-2-4(9)7-5/h1-2H,(H3,6,7,9)
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| Chemical Name |
6-amino-5-nitro-1H-pyridin-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~1.67 mg/mL (~10.77 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.4470 mL | 32.2352 mL | 64.4704 mL | |
| 5 mM | 1.2894 mL | 6.4470 mL | 12.8941 mL | |
| 10 mM | 0.6447 mL | 3.2235 mL | 6.4470 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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