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| Other Sizes |
| Targets |
5NAA does not have a single well-defined molecular target but is a biochemical compound used as a biological material or organic reagent for biomedical research. As a nitroaromatic compound, it may be reduced to aromatic amines in biological systems. Its mechanism of action may involve inhibition of bacterial enzymes or disruption of cellular processes. It is primarily used as a chemical intermediate and research reagent.
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| ln Vitro |
In vitro, 5NAA is used as a biochemical compound for biomedical research. As a nitroaromatic compound, it may exhibit antimicrobial activity against certain microorganisms. It is used as a starting material for the synthesis of dyes and other nitroaromatic compounds. Its in vitro properties are consistent with other nitroaromatic compounds used in research.
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| ln Vivo |
In vivo, 5NAA is not used as a therapeutic drug but is used as a research compound. As a nitroaromatic compound, it may be metabolized by nitroreductases to aromatic amines, which could have toxicological implications. Its primary applications are in chemical synthesis and biomedical research rather than as a therapeutic agent.
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| Enzyme Assay |
In non-cell-based biochemical assays, 5NAA is evaluated using standard biochemical methods. Its purity is assessed using HPLC (99.93%). Its solubility and other physicochemical properties are characterized. The compound may be used as a substrate or inhibitor in enzyme assays, particularly those involving nitroreductases. It is also used as a starting material for the synthesis of other compounds.
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| Cell Assay |
In vitro cellular assays for 5NAA involve testing its effects on cultured cells. The compound may be used to study the effects of nitroaromatic compounds on cell viability, proliferation, and oxidative stress. However, specific cellular assay data are limited, as 5NAA is primarily used as a chemical intermediate and research reagent rather than as a bioactive compound.
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| Animal Protocol |
In vivo animal studies for 5NAA have not been extensively reported, as the compound is primarily used as a chemical intermediate and research reagent rather than as a therapeutic agent. When studied, it is typically administered to animals to assess its metabolism, excretion, and potential toxicity. However, specific protocols and data are limited in publicly available sources.
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| ADME/Pharmacokinetics |
5NAA has a molecular weight of 182.13 and a molecular formula of C7H6N2O4. It is a light yellow to yellow solid powder with a density of 1.6±0.1 g/cm³. The compound is a nitroaromatic compound and is used as a starting material for the synthesis of dyes and other nitroaromatic compounds. It is a molecule secreted by Streptomyces scabies.
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| Toxicity/Toxicokinetics |
As a research compound, 5NAA is not intended for human use and should be handled with appropriate safety precautions. Nitroaromatic compounds may have toxicological effects, including mutagenicity and carcinogenicity. The compound should be stored and handled in accordance with standard laboratory safety guidelines. Comprehensive toxicology studies have not been extensively published.
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| Additional Infomation |
5-Nitro-o-aminobenzoic acid is an aminobenzoic acid in which the amino group is located ortho to the carboxyl group, and the para position of the amino group is replaced by a nitro group. It is the conjugate acid of 5-nitro-o-aminobenzoic acid ester. 2-Amino-5-nitrobenzoic acid has been reported in Streptomyces scabiei, and relevant data are available.
5NAA (2-Amino-5-nitrobenzoic acid, 5-nitroanthranilic acid) is a nitroaromatic compound with an amino group and a carboxylic acid group in the ortho position and a para-nitro substituent. It is used as a starting material for the synthesis of dyes and other nitroaromatic compounds. 5NAA is a molecule secreted by Streptomyces scabies. It is a biochemical compound used as a biological material or organic reagent for biomedical research. 5NAA is not an approved drug and is intended for research use only. |
| Molecular Formula |
C7H6N2O4
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|---|---|
| Molecular Weight |
182.135
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| Exact Mass |
182.032
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| CAS # |
616-79-5
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| PubChem CID |
12032
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
437.9±35.0 °C at 760 mmHg
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| Melting Point |
270 °C (dec.)(lit.)
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| Flash Point |
218.6±25.9 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
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| Index of Refraction |
1.682
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| LogP |
1.86
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
13
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| Complexity |
225
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC(=C(C=C1[N+](=O)[O-])C(=O)O)N
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| InChi Key |
RUCHWTKMOWXHLU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C7H6N2O4/c8-6-2-1-4(9(12)13)3-5(6)7(10)11/h1-3H,8H2,(H,10,11)
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| Chemical Name |
2-Amino-5-nitrobenzoic acid
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| Synonyms |
5 NAA 5-NAA5NAA
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4903 mL | 27.4514 mL | 54.9028 mL | |
| 5 mM | 1.0981 mL | 5.4903 mL | 10.9806 mL | |
| 10 mM | 0.5490 mL | 2.7451 mL | 5.4903 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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