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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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500mg |
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Other Sizes |
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5-Hydroxymethyldeoxyuridine is a nucleoside analog with potent anticancer and antiviral activities. It inhibits the replication of murine S180 lung carcinoma cells and Ehrlich ascites mammary carcinoma cells (ED50s = 8.5 and 4 μM, respectively) and multiple human leukemia cell lines (IC50s = 1.7-5.8 μM).
ln Vitro |
5-Hydroxymethyl-2'-deoxyuridine (0-10 μM) has an ED50 value of 8.5 μM for Ehrlich ascites cancer cells and 4 μM for sarcoma 180 cells, which both impede the multiplication of these cells [1]. Herpes simplex virus type 1 (HSV-1) pyrimidine 2'-deoxyribonucleoside kinase is inhibited by 5-hydroxymethyl-2'-deoxyuridine, with a Ki value of 3.5 μM [1]. 5-Hydroxymethyl-2'-deoxyuridine, with IC50 values ranging from 1.7 to 5.8 μM, inhibits the reproduction of several human leukemia cell lines[2]. Human acute promyelocytic leukemia cell lines are toxic to 5-hydroxymethyl-2'-deoxyuridine (10-100 μM) in a dose-dependent manner [2].
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ln Vivo |
The survival of DBA/2 mice harboring L1210 leukemia is increased by 5-hydroxymethyl-2'-deoxyuridine (0, 5 and 50 mg/kg; intraperitoneal injection; once) [3].
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References |
[1]. Shiau GT, et al. Synthesis and biological activities of 5-(hydroxymethyl, azidomethyl, or aminomethyl)-2'-deoxyuridine and related 5'-substituted analogues. J Med Chem. 1980 Feb;23(2):127-33.
[2]. Kahilainen LI, et al. 5-Hydroxymethyl-2'-deoxyuridine. Cytotoxicity and DNA incorporation studied by using a novel [2-14C]-derivative with normal and leukemic human hematopoietic cells. Acta Chem Scand B. 1985;39(6):477-84. [3]. JA Vilpo, et al. 5-Hydroxymethyl-2′-Deoxyuridine: Studies of Antileukemic Properties in Vitro and in Vivo. Nucleosides and Nucleotides . 1987. |
Molecular Formula |
C10H14N2O6
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Molecular Weight |
258.22796
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Exact Mass |
258.09
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Elemental Analysis |
C, 46.51; H, 5.46; N, 10.85; O, 37.17
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CAS # |
5116-24-5
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Appearance |
Solid powder
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SMILES |
OC[C@@H]1[C@H](C[C@H](N2C(NC(C(CO)=C2)=O)=O)O1)O
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InChi Key |
IPAVKOYJGUMINP-XLPZGREQSA-N
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InChi Code |
InChI=1S/C10H14N2O6/c13-3-5-2-12(10(17)11-9(5)16)8-1-6(15)7(4-14)18-8/h2,6-8,13-15H,1,3-4H2,(H,11,16,17)/t6-,7+,8+/m0/s1
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Chemical Name |
Uridine, 2'-deoxy-5-(hydroxymethyl)
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Synonyms |
5-Hydroxymethyldeoxyuridine; α-Hydroxythymidine; 2'-Deoxy-5-(hydroxymethyl)uridine; 5-Hydroxymethyl-2'-deoxyuridine
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~ 125 mg/mL (~484.06 mM)
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.8725 mL | 19.3626 mL | 38.7252 mL | |
5 mM | 0.7745 mL | 3.8725 mL | 7.7450 mL | |
10 mM | 0.3873 mL | 1.9363 mL | 3.8725 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.