| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Target: Isotope-Labeled Compounds / Endogenous Metabolite. 5alpha-Cholestane is a saturated steroidal hydrocarbon with a perhydrocyclopentanophenanthrene skeleton. It is a non-polar reference compound used in steroid chemistry. 5alpha-Cholestane is a precursor in the synthesis of brassinosteroids, plant hormones that regulate growth and development.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. No specific biological activity is reported for the labeled form. As an internal standard, it is chemically inert and does not bind to biological receptors. |
| ln Vivo |
In vivo, the labeled standard is not administered to animals for efficacy studies. The unlabeled 5alpha-Cholestane is a biologically inactive compound used solely as an analytical reference. Cholesterol, the precursor of 5alpha-Cholestane, is essential for membrane structure and steroid hormone synthesis, but the saturated hydrocarbon itself has no known biological activity.
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| Enzyme Assay |
For cell-free assay: plasma, serum, or tissue homogenates are saponified to release sterols, followed by lipid extraction (Folch method). The extracts are derivatized (e.g., to trimethylsilyl (TMS) ethers) and analyzed by GC-MS. 5alpha-Cholestane-d6 is spiked into the samples as an internal standard prior to processing to correct for losses during extraction and derivatization and to provide a stable reference for quantitation of cholesterol and other sterols.
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| Cell Assay |
No cell-based assays are performed with the labeled standard. The unlabeled 5alpha-Cholestane is not used in cell culture because it is a highly hydrophobic hydrocarbon that is not metabolized or incorporated into biological membranes. It is purely an analytical reference compound for sterol analysis, not a cell-penetrating active molecule.
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| Animal Protocol |
No animal studies are conducted with the labeled internal standard itself. For cholesterol metabolism studies, animals are fed a normal or high-cholesterol diet, and tissues (liver, plasma, aorta) are collected. 5alpha-Cholestane-d6 is added as an internal standard during sample processing for GC-MS analysis to quantify cholesterol and its metabolites, but the compound itself is not administered to the animals.
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| ADME/Pharmacokinetics |
PK properties are not reported for 5alpha-Cholestane-d6, as it is not a drug and is not absorbed or metabolized in a pharmacological sense. The compound is used as an external standard for the quantitative analysis of cholesterol. It is chemically stable and does not undergo metabolism when used as an internal standard in processed biological samples. Its XLogP3 is 11.1, indicating extreme hydrophobicity.
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| Toxicity/Toxicokinetics |
No toxicity data are available for this specific compound. 5alpha-Cholestane is considered to have low acute toxicity because it is poorly absorbed and is not biologically active. The deuterated standard is for research use only and is not intended for human consumption. The compound is stable if stored under recommended conditions (room temperature, dry). Non-hazardous for transport.
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| References | |
| Additional Infomation |
C27-steroid is a C27 steroid.
See also: cholesterane (note moved to); coprosterane (note moved to). 5alpha-Cholestane-d6 is a research standard, not a drug. It is not FDA-approved for clinical use. It is used as an internal standard for cholesterol quantification in clinical diagnostics, lipidomics, and nutritional research. The unlabeled 5alpha-Cholestane is used in the synthesis of brassinosteroids for agricultural research. The labeled compound is essential for stable isotope dilution assays in mass spectrometry. |
| Molecular Formula |
C27H42D6
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|---|---|
| Molecular Weight |
378.71
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| Exact Mass |
378.413
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| CAS # |
358731-18-7
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| PubChem CID |
10202
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| Appearance |
White to off-white solid powder
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| LogP |
8.497
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
0
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
27
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| Complexity |
506
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
XIIAYQZJNBULGD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C27H48/c1-19(2)9-8-10-20(3)23-14-15-24-22-13-12-21-11-6-7-17-26(21,4)25(22)16-18-27(23,24)5/h19-25H,6-18H2,1-5H3
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| Chemical Name |
10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthrene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6405 mL | 13.2027 mL | 26.4054 mL | |
| 5 mM | 0.5281 mL | 2.6405 mL | 5.2811 mL | |
| 10 mM | 0.2641 mL | 1.3203 mL | 2.6405 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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