| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
5α-7,24-cholestadiene is an oxygenated derivatives of cholesterol (oxysterols) which present a remarkably diverse profile of biological activities, including effects on sphingolipid metabolism, platelet aggregation, apoptosis, protein prenylation, and cholesterol homeostasis.
| Targets |
Not applicable (biochemical reagent; interacts with lipid membranes).
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|---|---|
| ln Vitro |
No specific pharmacological activity reported; 5alpha-7,24-cholestadiene is primarily a sterol standard. As an intermediate in cholesterol synthesis, it can be used to trace the mevalonate pathway in cells. Its double bond structure affects its behavior in lipid bilayers compared to saturated sterols, potentially influencing membrane fluidity and permeability.
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| ln Vivo |
No specific in vivo activity reported; 5alpha-7,24-cholestadiene is typically used ex vivo to spike biological samples for analytical method validation. As a reference standard, it is not administered to animals for pharmacodynamic evaluation. Instead, its presence in tissues or food products is quantified after extraction and analysis by GC or LC-MS.
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| Enzyme Assay |
Assay: Lipid extraction and GC analysis. Protocol: Biological samples (e.g., plasma, tissues, or food) are saponified to release sterols. Lipids are extracted using organic solvents (e.g., chloroform/methanol). The extract is derivatized (e.g., trimethylsilylation) to improve volatility. Samples are analyzed by GC with a DB-5 or similar capillary column and flame ionization detection (FID) or mass spectrometry (MS). Retention time and mass spectrum are compared to the authentic standard.
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| Cell Assay |
No specific cell-based assays; 5alpha-7,24-cholestadiene is typically not used in cell culture experiments to study pharmacological effects. It is not used to treat cells for viability or functional assays. Instead, it is processed analytically: cells grown in culture are harvested, lipids are extracted, and the content of 5alpha-7,24-cholestadiene is quantified by GC-MS to evaluate sterol biosynthesis or metabolic flux.
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| Animal Protocol |
No specific in vivo protocol; 5alpha-7,24-cholestadiene is not administered to animals as a therapeutic agent. It is used as an analytical standard to quantify sterol intermediates in biological samples. For in vivo studies, animals (e.g., rodents) may be fed a controlled diet, and tissues (liver, plasma) are collected post-mortem. Sterols are extracted and analyzed by GC-MS, with the compound used as an external standard to calibrate the instrument.
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| ADME/Pharmacokinetics |
No specific PK data; 5alpha-7,24-cholestadiene is an endogenous intermediate and is not a drug candidate. In animal models, its concentration in plasma or tissues reflects cholesterol biosynthesis rather than exogenous administration. It is hydrophobic and would likely be incorporated into lipoproteins and transported in the bloodstream if administered exogenously, similar to cholesterol.
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| Toxicity/Toxicokinetics |
No specific toxicity data; 5alpha-7,24-cholestadiene is an endogenous sterol that is a natural intermediate in the cholesterol synthesis pathway. At normal physiological concentrations, it is not toxic. When used as an analytical standard, it is handled in minimal quantities (mg-scale) and poses little risk. Standard laboratory safety precautions (gloves, eye protection) should be followed to avoid skin/eye contact.
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| References | |
| Additional Infomation |
5α-Cholesterol-7,24-dien-3β-ol is a 3β-sterol. It is a metabolite in both mice and humans. It is derived from the hydrogenation of 5α-cholestane. Reports have indicated the detection of 5α-cholestane-7,24-dien-3β-ol in Homo sapiens, and relevant data are available for reference.
5alpha-7,24-cholestadiene (CAS#: 651-54-7) is a sterol standard. It is often used in combination with other sterol standards (e.g., cholesterol, desmosterol, lathosterol) to study the entire cholesterol biosynthesis pathway. The compound is not a drug and is not intended for human use. It is strictly a research reagent for analytical chemistry and lipid metabolism studies. |
| Molecular Formula |
C27H44O
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|---|---|
| Molecular Weight |
384.63766
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| Exact Mass |
384.339
|
| CAS # |
651-54-7
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| PubChem CID |
5459827
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| Appearance |
White to off-white solid powder
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| Density |
1g/cm3
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| Boiling Point |
481.9ºC at 760 mmHg
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| Melting Point |
147 - 151 °C
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| Flash Point |
208.9ºC
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| Index of Refraction |
1.535
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| LogP |
7.308
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
1
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
28
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| Complexity |
641
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| Defined Atom Stereocenter Count |
8
|
| SMILES |
C[C@H](CCC=C(C)C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3C2=CC[C@@H]4[C@@]3(CC[C@@H](C4)O)C)C
|
| InChi Key |
PKEPPDGGTSZLBL-SKCNUYALSA-N
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| InChi Code |
InChI=1S/C27H44O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h7,10,19-21,23-25,28H,6,8-9,11-17H2,1-5H3/t19-,20+,21+,23-,24+,25+,26+,27-/m1/s1
|
| Chemical Name |
(3S,5S,9R,10S,13R,14R,17R)-10,13-dimethyl-17-[(2R)-6-methylhept-5-en-2-yl]-2,3,4,5,6,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~1.43 mg/mL (~3.72 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5998 mL | 12.9992 mL | 25.9983 mL | |
| 5 mM | 0.5200 mL | 2.5998 mL | 5.1997 mL | |
| 10 mM | 0.2600 mL | 1.2999 mL | 2.5998 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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