| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| ln Vitro |
Because of their advantageous photochemical and photophysical characteristics, rhodamine dyes are significant. They possess high molar absorptivity, long wavelength absorption maxima, and high quantum yield [1].
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| References | |
| Additional Infomation |
5-Carboxy-X-Rhodamine is an X-rhodamine compound with a carboxyl substituent at the 5-position. It is a fluorescent dye. It is derived from the hydride of 2,3,6,7,12,13,16,17-octahydropyridino[3,2,1-ij]quinolino[1',9':6,7,8]chromeno[2,3-f]quinoline-18-onium.
5-ROX (5-carboxy-X-rhodamine) is a member of the carboxy-X-rhodamine (ROX) fluorescent dye family, characterized by the regioselective introduction of a carboxyl group at the 5-position of its rhodamine core structure [1] The synthesis of 5-ROX follows a regioselective carboxylation strategy: X-rhodamine is used as a precursor, dissolved in an aprotic polar solvent, and reacted with a carboxylating agent at room temperature for 12-16 hours under an inert gas atmosphere. The reaction is specifically targeted at the 5-position of the rhodamine skeleton, minimizing the formation of the 6-position isomer [1]. The crude 5-ROX was purified by silica gel column chromatography using a gradient elution system consisting of low- to high-polarity organic solvents. High-performance liquid chromatography (HPLC) analysis confirmed that the purity of the final product exceeded 98% [1]. The structure of 5-ROX was verified by a variety of spectroscopic techniques: ¹H nuclear magnetic resonance (¹H NMR) and ¹³C NMR spectroscopy confirmed the correct position of the 5-carboxyl group and the integrity of the rhodamine skeleton; mass spectrometry (MS) provided the precise molecular weight and verified its chemical structure [1]. The carboxyl group of 5-ROX can be covalently bound to the primary amines of biomolecules (e.g., oligonucleotides, peptides, proteins) through the formation of amide bonds. This functional property makes it a highly promising tool in fluorescence applications such as bioimaging, immunoassay, and molecular labeling [1]. 5-ROX has typical photophysical properties of rhodamine dyes, including strong absorption and emission in the visible spectrum, which is consistent with the structural characteristics of the ROX family [1]. |
| Molecular Formula |
C33H30N2O5
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|---|---|
| Molecular Weight |
534.60200
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| Exact Mass |
534.215
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| CAS # |
216699-35-3
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| PubChem CID |
4097103
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| Appearance |
Light brown to black solid powder
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| LogP |
5.123
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
40
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| Complexity |
1200
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
UNGMOMJDNDFGJG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C33H30N2O5/c36-32(37)20-9-10-21(24(17-20)33(38)39)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39)
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| Chemical Name |
3-carboxy-4-(3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaen-16-yl)benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~233.82 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8706 mL | 9.3528 mL | 18.7056 mL | |
| 5 mM | 0.3741 mL | 1.8706 mL | 3.7411 mL | |
| 10 mM | 0.1871 mL | 0.9353 mL | 1.8706 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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