| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 100mg | |||
| Other Sizes |
| Targets |
5-MethoxyPinocembroside is classified as a flavonoid, a class of polyphenolic compounds with diverse biological activities. As a natural product extracted from Penthorum chinense Pursh, it may interact with various cellular targets involved in inflammation, oxidative stress, and metabolic regulation. Detailed molecular targets are not extensively characterized.
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| ln Vitro |
In vitro activity data for 5-MethoxyPinocembroside are not extensively documented. As a flavonoid from Penthorum chinense Pursh, the compound is expected to exhibit antioxidant, anti-inflammatory, or other biological activities characteristic of flavonoids. Detailed IC₅0 or EC₅0 values are not available in the public literature. Further studies would be needed to characterize its bioactivity.
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| ln Vivo |
In vivo activity data for 5-MethoxyPinocembroside are not extensively documented. As a natural flavonoid, the compound may have potential applications in inflammation and metabolic research. Further in vivo studies would be needed to characterize its pharmacokinetic and efficacy profile.
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| Enzyme Assay |
The in vitro assay for flavonoid activity typically involves measuring antioxidant capacity using DPPH, ABTS, or FRAP assays. Anti-inflammatory activity may be assessed by measuring inhibition of pro-inflammatory cytokine production (such as TNF-alpha, IL-6) in LPS-stimulated macrophages. Cells are treated with varying concentrations of 5-MethoxyPinocembroside, and activity is measured using colorimetric or ELISA-based methods. IC₅0 values are calculated from dose-response curves.
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| Cell Assay |
For in vitro cell-based assays, macrophages or other cell lines are cultured and treated with 5-MethoxyPinocembroside at various concentrations. Cell viability is measured using MTT or other proliferation assays. Anti-inflammatory activity is assessed by measuring cytokine levels (TNF-alpha, IL-6, IL-1beta) in culture supernatants by ELISA. Antioxidant activity may be assessed by measuring intracellular ROS levels using fluorescent probes. Experiments are typically performed in triplicate.
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| Animal Protocol |
In vivo animal studies for 5-MethoxyPinocembroside would typically involve mouse models of inflammation (such as LPS-induced or carrageenan-induced inflammation). The compound is administered orally or intraperitoneally at various doses. Inflammatory markers are measured in serum or tissue samples. Antioxidant parameters (such as MDA, SOD, GSH) may be measured in tissues. Efficacy is evaluated by reduction in inflammatory or oxidative stress parameters.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-MethoxyPinocembroside are not extensively documented. The compound has molecular formula C22H24O₉ and molecular weight 432.4 g/mol. It is soluble in DMSO and typically formulated for in vitro studies. Purity is typically >98% (HPLC). Storage at -20degC under desiccated conditions is recommended. Further PK parameters would require dedicated studies.
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| Toxicity/Toxicokinetics |
Toxicological data for 5-MethoxyPinocembroside are not well characterized. As a natural flavonoid, standard safety precautions should be observed. The compound is for laboratory use only and not intended for human therapeutic applications. Flavonoids as a class are generally considered to have favorable safety profiles. Comprehensive toxicity profiling would be required for therapeutic development.
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| Additional Infomation |
5-MethoxyPinocembroside (CAS# 1450878-89-3) is a flavonoid extracted from Penthorum chinense Pursh. It has molecular formula C22H24O₉ and molecular weight 432.4 g/mol. The compound is used in natural product research and is not approved for clinical use.
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| Molecular Formula |
C22H24O9
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|---|---|
| Molecular Weight |
432.420567512512
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| Exact Mass |
432.142
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| CAS # |
1450878-89-3
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| PubChem CID |
21580051
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0.7
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
31
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| Complexity |
610
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| Defined Atom Stereocenter Count |
6
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| SMILES |
COC1=CC(=CC2=C1C(=O)C[C@H](O2)C3=CC=CC=C3)O[C@H]4[C@@H]([C@H]([C@@H]([C@H](O4)CO)O)O)O
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| InChi Key |
RRNVUIVTKIOLBO-YMTXFHFDSA-N
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| InChi Code |
InChI=1S/C22H24O9/c1-28-15-7-12(29-22-21(27)20(26)19(25)17(10-23)31-22)8-16-18(15)13(24)9-14(30-16)11-5-3-2-4-6-11/h2-8,14,17,19-23,25-27H,9-10H2,1H3/t14-,17+,19+,20-,21+,22+/m0/s1
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| Chemical Name |
(2S)-5-methoxy-2-phenyl-7-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2,3-dihydrochromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.3126 mL | 11.5628 mL | 23.1257 mL | |
| 5 mM | 0.4625 mL | 2.3126 mL | 4.6251 mL | |
| 10 mM | 0.2313 mL | 1.1563 mL | 2.3126 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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