| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg | |||
| Other Sizes |
| Targets |
5-DTAF does not have a specific biological target as a drug. Its primary application is as a fluorescent labeling reagent for polysaccharides and other alcohols. The dichlorotriazine group reacts with hydroxyl groups to form covalent bonds. This reactivity is exploited to label carbohydrates, glycoproteins, and other hydroxyl-containing biomolecules. The fluorescein moiety provides bright green fluorescence upon excitation, enabling detection and tracking of the labeled molecules.
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| ln Vitro |
In vitro, 5-DTAF is a fluorescein isomer derivative that reacts directly with polysaccharides and other alcohols in aqueous solutions at pH > 9. Upon excitation at 488 nm, its maximum emission line is at 518 nm. The compound is used as a fluorescent label to track biomolecules in cells and tissues. Its reactivity with hydroxyl groups allows for the labeling of a wide range of biomolecules.
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| ln Vivo |
In vivo activity of 5-DTAF is not typically studied, as it is a fluorescent labeling reagent rather than a bioactive drug. However, the labeled biomolecules (e.g., labeled polysaccharides) could be used in vivo for imaging or tracking purposes. The dye's green fluorescence would allow for detection using standard fluorescence imaging systems.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays using 5-DTAF typically involve labeling polysaccharides or other hydroxyl-containing compounds with the dye. A standard protocol for labeling a polysaccharide: the polysaccharide is dissolved in a buffer at pH > 9. 5-DTAF is added to the polysaccharide solution, and the reaction mixture is incubated at room temperature for several hours. The labeled polysaccharide is purified by dialysis or precipitation. The degree of labeling is determined by measuring the absorbance at 488 nm and the polysaccharide concentration.
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| Cell Assay |
In vitro cell-based assays using 5-DTAF are not common, as it is primarily used for labeling extracellular biomolecules. However, it could be used to label cell surface carbohydrates. A standard protocol: cells are harvested and incubated with 5-DTAF in a buffer at pH > 9. The cells are washed to remove unreacted dye. The labeled cells can be analyzed by flow cytometry to assess the expression of specific carbohydrates on the cell surface. Alternatively, the labeled cells can be visualized by fluorescence microscopy.
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| Animal Protocol |
In vivo animal experiments for 5-DTAF are not typically performed for the compound itself. However, 5-DTAF-labeled biomolecules could be administered to animals for tracking purposes. For example, a 5-DTAF-labeled polysaccharide could be injected intravenously, and its distribution in the body could be monitored by measuring the fluorescence in blood and tissues. Such studies would require careful consideration of the dye's properties and the stability of the labeled molecule.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-DTAF are not a primary focus of study. The compound is a reactive labeling reagent that is typically used in vitro. Once conjugated to a biomolecule, the pharmacokinetics of the conjugate are determined by the biomolecule rather than the dye. The compound is typically stored as a powder and protected from light.
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| Toxicity/Toxicokinetics |
Toxicity data for 5-DTAF is limited. As a chemical reagent, it should be handled with standard laboratory precautions. The compound contains a dichlorotriazine group, which is reactive and may cause irritation. Inhalation, ingestion, and skin contact should be avoided. Appropriate personal protective equipment should be worn.
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| References | |
| Additional Infomation |
5-DTAF is a fluorescein isomer derivative that reacts directly with polysaccharides and other alcohols in aqueous solutions at pH > 9. It has an excitation at 488 nm and emission at 518 nm. It is used as a fluorescent label to track biomolecules. It is not a drug and has no clinical use or approval status. It is commercially available from chemical suppliers for research purposes only.
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| Molecular Formula |
C23H12CL2N4O5
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|---|---|
| Molecular Weight |
495.2712
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| Exact Mass |
529.995
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| CAS # |
51306-35-5
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| Related CAS # |
21811-74-5 (mono-hydrochloride);68425-77-4 (di-hydrochloride)
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| PubChem CID |
123934
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.81g/cm3
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| Boiling Point |
834.4ºC at 760mmHg
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| Melting Point |
>325ºC
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| Flash Point |
458.4ºC
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| LogP |
6.079
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
34
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| Complexity |
759
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HWQQCFPHXPNXHC-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C23H12Cl2N4O5/c24-20-27-21(25)29-22(28-20)26-10-1-4-14-13(7-10)19(32)34-23(14)15-5-2-11(30)8-17(15)33-18-9-12(31)3-6-16(18)23/h1-9,30-31H,(H,26,27,28,29)
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| Chemical Name |
6-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~100.96 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0191 mL | 10.0955 mL | 20.1910 mL | |
| 5 mM | 0.4038 mL | 2.0191 mL | 4.0382 mL | |
| 10 mM | 0.2019 mL | 1.0096 mL | 2.0191 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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