| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Lipid bilayers, cell membranes, and detergent micelles. The compound integrates into hydrophobic environments via its C12 acyl chain, while the fluorescein moiety provides fluorescence for detection and imaging.
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|---|---|
| ln Vitro |
The fluorescence of 5-dodecanoylaminofluorescein (λex=490-495 nm, λem=515-520 nm) is sensitive to the polarity of its environment. It has been used to analyze membrane fluidity, assess the critical micelle concentration (CMC) of detergents, and prepare hydrophobic nanospheres for drug delivery.
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| ln Vivo |
Not applicable; this is an imaging/diagnostic probe, not a therapeutic agent. It is not administered in vivo as a drug. It may be used for ex vivo imaging of tissues or for preparing fluorescent nanoparticles.
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| Enzyme Assay |
5-Dodecanoylaminofluorescein is dissolved in DMSO at 1-10 mM. Various concentrations (0.1-100 microM) are added to detergent solutions (e.g., SDS, Triton X-100, 0.001-10%). Fluorescence intensity is measured (λex=495 nm, λem=520 nm) to determine the CMC. Alternatively, anisotropy is measured to assess membrane fluidity.
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| Cell Assay |
Cells (e.g., HeLa, Jurkat, 1×10^6 cells) are incubated with 5-dodecanoylaminofluorescein (0.1-10 microM) in serum-free media for 15-60 minutes at 37degC. After washing, cells are analyzed by flow cytometry (FL1 channel) or fluorescence microscopy to visualize membrane labeling.
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| Animal Protocol |
Not applicable as a drug. For nanoparticle studies, 5-dodecanoylaminofluorescein is incorporated into PLGA or lipid nanoparticles by solvent evaporation. The fluorescent nanoparticles (10-100 microg/mL in PBS) are injected intravenously into mice (50-200 microL) and biodistribution is imaged ex vivo.
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| ADME/Pharmacokinetics |
Not applicable for the free probe. When incorporated into nanoparticles, the encapsulated probe exhibits a circulation half-life of 1-6 hours depending on nanoparticle size and surface coating. The free probe is rapidly cleared from plasma (t1/2 < 5 min).
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| Toxicity/Toxicokinetics |
No formal toxicity data. The compound is typically used at low concentrations (0.1-10 microM) for cell labeling and shows minimal cytotoxicity in short-term studies. As with any fluorescent dye, avoid prolonged light exposure to prevent photobleaching.
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| References |
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| Additional Infomation |
5-Dodecanoylaminofluorescein is for research use only, not clinically approved. It is a valuable tool for studying membrane organization, liposome stability, and drug delivery systems. It is also known as DAF (dodecanoylaminofluorescein) or AFC12.
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| Molecular Formula |
C32H35NO6
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|---|---|
| Molecular Weight |
529.6234
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| Exact Mass |
529.246
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| CAS # |
107827-77-0
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| PubChem CID |
147135
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.3g/cm3
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| Boiling Point |
773.7ºC at 760mmHg
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| Flash Point |
421.7ºC
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| Vapour Pressure |
8.28E-25mmHg at 25°C
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| Index of Refraction |
1.648
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| LogP |
8.174
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
11
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| Heavy Atom Count |
39
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| Complexity |
808
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VJNRUQFWKRLMSL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C32H35NO6/c1-2-3-4-5-6-7-8-9-10-11-30(36)33-21-12-15-25-24(18-21)31(37)39-32(25)26-16-13-22(34)19-28(26)38-29-20-23(35)14-17-27(29)32/h12-20,34-35H,2-11H2,1H3,(H,33,36)
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| Chemical Name |
N-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)dodecanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8881 mL | 9.4407 mL | 18.8815 mL | |
| 5 mM | 0.3776 mL | 1.8881 mL | 3.7763 mL | |
| 10 mM | 0.1888 mL | 0.9441 mL | 1.8881 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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