| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
5-BrUTP sodium salt targets nascent RNA during transcription. It serves as a substrate for RNA polymerases and is incorporated into newly synthesized RNA in place of uridine triphosphate (UTP). The bromine atom provides a unique epitope that can be detected immunologically with specific antibodies. This enables visualization and quantification of RNA synthesis in cells.
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| ln Vitro |
5-BrUTP sodium salt is used as a labeling reagent for measuring RNA transcription in vitro. The compound is incorporated into RNA during transcription reactions. Incorporated 5-BrUTP can be detected immunologically with antibodies. The labeling does not significantly interfere with transcription or RNA function. The compound is widely used in studies of RNA synthesis, processing, and dynamics.
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| ln Vivo |
5-BrUTP sodium salt is used in vivo for labeling RNA to measure transcription. RNA or cells labeled via 5-BrUTP incorporation may be detected immunologically with antibodies. The compound can be delivered to cells or tissues for labeling nascent RNA. This allows monitoring of RNA synthesis and processing in living cells and tissues. The labeling approach is widely used in studies of transcriptional regulation.
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| Enzyme Assay |
In vitro transcription assays using 5-BrUTP sodium salt involve setting up transcription reactions with RNA polymerase, template DNA, nucleoside triphosphates (including 5-BrUTP instead of UTP), and appropriate buffer conditions. Reactions are incubated at appropriate temperature (typically 37degC) for 1-2 hours. The resulting 5-BrUTP-labeled RNA can be detected by dot blot, Northern blot, or ELISA using anti-BrUTP antibodies.
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| Cell Assay |
In vitro cellular assays for 5-BrUTP sodium salt involve treating cells with the compound to label newly synthesized RNA. Cells are incubated with 5-BrUTP (typically 0.1-1 mM range) for 1-4 hours. Labeled RNA is detected by immunocytochemistry using anti-BrUTP antibodies, followed by fluorescence microscopy. Alternatively, labeled RNA can be purified and quantified by ELISA or detected by flow cytometry. Incorporation efficiency is assessed by comparison with controls.
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| Animal Protocol |
In vivo animal studies with 5-BrUTP sodium salt typically involve administration of the compound to label nascent RNA in tissues. The compound can be injected locally or systemically, and tissues are harvested at various time points. Labeled RNA is detected by immunohistochemistry using anti-BrUTP antibodies. This approach enables assessment of transcriptional activity in specific tissues or cell types. The compound is not used for therapeutic purposes.
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| ADME/Pharmacokinetics |
5-BrUTP sodium salt is a water-soluble nucleotide that is typically provided as a solution in water. Following administration, the compound is taken up by cells and incorporated into RNA. The compound's stability and labeling efficiency depend on experimental conditions. The sodium salt form enhances solubility and stability. The compound is typically stored at -20degC. It is for research use only and not for human use.
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| Toxicity/Toxicokinetics |
5-BrUTP sodium salt is generally considered safe for research applications. As a modified nucleotide, standard laboratory safety precautions should be observed including appropriate PPE. The compound is not intended for therapeutic use. It is used for research purposes only. Comprehensive toxicology data is not available as the compound is not intended for human use. Proper disposal procedures should be followed according to institutional guidelines.
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| References | |
| Additional Infomation |
5-BrUTP sodium salt (CAS# 161848-60-8) is a modified nucleotide used to label RNA and measure transcription. It is also known as 5-bromouridine 5'-triphosphate sodium salt or BrUTP. This compound is widely used in scientific research to study RNA transcription and processing. RNA or cells labeled via 5-BrUTP incorporation may be detected immunologically with antibodies. The compound is for research use only and not for human use.
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| Molecular Formula |
C9H11N2O15P3BR-3.3[NA+]
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|---|---|
| Molecular Weight |
628.98284
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| Exact Mass |
685.809
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| CAS # |
161848-60-8
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| PubChem CID |
168012295
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
31
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| Complexity |
889
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| Defined Atom Stereocenter Count |
4
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~33.33 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5899 mL | 7.9494 mL | 15.8988 mL | |
| 5 mM | 0.3180 mL | 1.5899 mL | 3.1798 mL | |
| 10 mM | 0.1590 mL | 0.7949 mL | 1.5899 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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