| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 100mg | |||
| Other Sizes |
| Targets |
5-BrdUTP sodium salt targets DNA, specifically sites of DNA double-strand breaks. As a substrate for TdT, it is incorporated into the 3'-hydroxyl termini of DNA fragments, allowing for the detection and labeling of DNA damage.
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|---|---|
| ln Vitro |
In vitro, 5-BrdUTP sodium salt is used as a labeling substrate for TdT reactions. It is utilized to mark broken DNA double strands. Its activity is assessed by its incorporation into DNA fragments by TdT, which can be detected by various methods.
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| ln Vivo |
In vivo, 5-BrdUTP sodium salt is not used as a therapeutic agent but as a research tool. It could be used in animal models to label cells with DNA damage, but its primary application is in in vitro biochemical and cellular assays.
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| Enzyme Assay |
In vitro assays for 5-BrdUTP sodium salt are based on its function as a TdT substrate. The assay involves incubating the compound with TdT and a DNA substrate, and measuring the incorporation of the labeled nucleotide into the DNA. This can be detected by fluorescence or other detection methods.
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| Cell Assay |
Cellular assays for 5-BrdUTP sodium salt are used to detect DNA double-strand breaks in cells. Cells are fixed and permeabilized, and then incubated with TdT and 5-BrdUTP. The incorporated nucleotide is then detected with an anti-BrdU antibody, allowing for the visualization and quantification of DNA damage.
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| Animal Protocol |
In vivo animal studies for 5-BrdUTP sodium salt are not typical. As a research tool, it is primarily used in in vitro assays. It could potentially be used in vivo for labeling DNA damage, but such applications are not well-documented.
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| ADME/Pharmacokinetics |
The pharmacokinetic properties of 5-BrdUTP sodium salt are not relevant for its intended use as a research reagent. It has a molecular weight of 547.04 g/mol (free acid). It is provided as a solution in water and is stored at -20degC.
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| Toxicity/Toxicokinetics |
The toxicological data for 5-BrdUTP sodium salt are limited. It is a research reagent and is not intended for human therapeutic use. Standard safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
5-BrdUTP sodium salt is a TdT substrate used to label DNA double-strand breaks. It is also known as 5-Bromo-2'-deoxyuridine 5'-triphosphate sodium salt. It is used in DNA synthesis and TdT reactions and is available for research use only.
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| Molecular Formula |
C9H10N2O14P3BR-4.4[NA+]
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|---|---|
| Molecular Weight |
634.9653
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| Exact Mass |
545.884
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| CAS # |
102212-99-7
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| PubChem CID |
65267
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
-4.1
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
29
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| Complexity |
857
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O=C(N1)N([C@@H]2O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)C2)C=C(Br)C1=O.[4Na]
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| InChi Key |
BLQCQNFLEGAHPA-RRKCRQDMSA-N
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| InChi Code |
InChI=1S/C9H14BrN2O14P3/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18)/t5-,6+,7+/m0/s1
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| Chemical Name |
[[(2R,3S,5R)-5-(5-bromo-2,4-dioxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5749 mL | 7.8744 mL | 15.7488 mL | |
| 5 mM | 0.3150 mL | 1.5749 mL | 3.1498 mL | |
| 10 mM | 0.1575 mL | 0.7874 mL | 1.5749 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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