| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg |
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| 500mg | |||
| Other Sizes |
| Targets |
The primary targets of 5-Geranoxy-7-methoxycoumarin include inflammatory pathways, microbial pathogens, and cancer cell lines. It has been shown to exhibit anti-inflammatory activity by modulating cytokine production and inhibiting inflammatory mediators. The compound also demonstrates antimicrobial activity against various bacterial and fungal strains. Its anticancer potential is attributed to its ability to induce apoptosis and inhibit cell proliferation in cancer cells.
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| ln Vitro |
In SW480 cells, 5-geranoxy-7-methoxycoumarin (12.5-200 µM; 24-72 hours) significantly inhibits cell proliferation in a time-dependent fashion[1]. 5-Geranoxy-7-methoxycoumarin (50 µM; 48 hours) inhibits the p38 MAPK signaling pathway in SW480 cells, which leads to DNA fragmentation and apoptosis[1]. After 48 hours of incubation, the percentage of cells in the sub G1/G0 phase rises from 5.3% (in control cells) to 18% when 5-geranoxy-7-methoxycoumarin (50 µM) is added[1].
In vitro, 5-Geranoxy-7-methoxycoumarin has been reported to possess anti-inflammatory, antimicrobial, and anticancer activities. Studies have shown that it can inhibit the production of pro-inflammatory cytokines and reduce the activation of NF-κB signaling. The compound also exhibits inhibitory effects against the growth of several Gram-positive and Gram-negative bacteria, as well as fungi. In cancer cell lines, it induces cell cycle arrest and apoptosis. |
| ln Vivo |
In vivo activity of 5-Geranoxy-7-methoxycoumarin has not been extensively characterized. As a natural coumarin derivative, it has potential therapeutic applications in the treatment of inflammatory diseases, infections, and cancer. However, detailed in vivo pharmacokinetic and pharmacodynamic studies are lacking. The compound is primarily studied for its in vitro biological activities.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for 5-Geranoxy-7-methoxycoumarin are not well established, as it is a natural product with multiple activities. Standard assays include anti-inflammatory activity using LPS-stimulated macrophages and measuring cytokine levels. Antimicrobial activity is assessed by broth microdilution to determine MIC values. Anticancer activity is evaluated by MTT assays on cancer cell lines. The compound is typically tested in a concentration range of 1-100 µM.
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| Cell Assay |
Cell Proliferation Assay [1]
Cell Types: SW480 Tested Concentrations: 0-20 μM Incubation Duration: 24, 48 and 72 hrs (hours) Experimental Results: Inhibited cell growth by 67% at 72 hrs (hours). Apoptosis Analysis[1] Cell Types: SW480 cells Tested Concentrations: 50 μM Incubation Duration: 48 hrs (hours) Experimental Results: Induced cell apoptosis. Cell Cycle Analysis[1] Cell Types: SW480 cells Tested Concentrations: 50 μM Incubation Duration: 48 hrs (hours) Experimental Results: Inhibited cell cycle progression in vitro. In vitro cellular assays for 5-Geranoxy-7-methoxycoumarin involve testing its effects on immune cells, microbial cultures, and cancer cells. For anti-inflammatory studies, macrophages are stimulated with LPS and treated with the compound; cytokine levels are measured by ELISA. For antimicrobial studies, bacterial and fungal cultures are treated with serial dilutions, and growth inhibition is monitored. For anticancer studies, cancer cell lines are treated, and cell viability and apoptosis are assessed. |
| Animal Protocol |
In vivo animal studies for 5-Geranoxy-7-methoxycoumarin are limited. For anti-inflammatory efficacy, animal models of inflammation (e.g., carrageenan-induced paw edema) could be used. For antimicrobial efficacy, infection models could be applied. For anticancer efficacy, tumor xenograft models could be employed. Animals would be treated with the compound via oral or intraperitoneal administration, and relevant endpoints would be measured.
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| ADME/Pharmacokinetics |
5-Geranoxy-7-methoxycoumarin has a molecular formula of C20H24O4 and a molecular weight of 328.40. It appears as a solid with a purity of ≥98%. It is soluble in organic solvents such as DMSO, ethanol, and methanol. The compound should be stored in a dry, dark place at -20°C. Stability information in solution is limited, and it is recommended to prepare fresh stock solutions. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 5-Geranoxy-7-methoxycoumarin has not been extensively characterized. As a natural coumarin, it is generally considered to have low to moderate toxicity. Standard toxicity studies would include acute oral toxicity, dermal irritation, and repeated-dose toxicity in animal models. The compound is intended for research use only and is not approved for clinical use.
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| References |
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| Additional Infomation |
5-Germanoxy-7-methoxycoumarin is a terpene lactone.
5-Geranoxy-7-methoxycoumarin (CAS 7380-39-4) is a naturally occurring coumarin derivative found in various plants. It has a molecular formula of C20H24O4 and a molecular weight of 328.40. The compound has been studied for its anti-inflammatory, antimicrobial, and anticancer activities. It exhibits inhibition of pro-inflammatory cytokines, antimicrobial effects against various pathogens, and antiproliferative effects on cancer cells. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C20H24O4
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|---|---|
| Molecular Weight |
328.40
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| Exact Mass |
328.167
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| CAS # |
7380-39-4
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| PubChem CID |
6441377
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.092g/cm3
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| Boiling Point |
487.1ºC at 760 mmHg
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| Melting Point |
86-88ºC
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| Flash Point |
213.2ºC
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| Index of Refraction |
1.542
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| LogP |
4.873
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
24
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| Complexity |
514
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=CCCC(=CCOC1=CC(=CC2=C1C=CC(=O)O2)OC)C)C
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| InChi Key |
WXUOSNJWDJOHGW-XNTDXEJSSA-N
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| InChi Code |
InChI=1S/C20H24O4/c1-14(2)6-5-7-15(3)10-11-23-18-12-16(22-4)13-19-17(18)8-9-20(21)24-19/h6,8-10,12-13H,5,7,11H2,1-4H3/b15-10+
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| Chemical Name |
5-[(2E)-3,7-dimethylocta-2,6-dienoxy]-7-methoxychromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0451 mL | 15.2253 mL | 30.4507 mL | |
| 5 mM | 0.6090 mL | 3.0451 mL | 6.0901 mL | |
| 10 mM | 0.3045 mL | 1.5225 mL | 3.0451 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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