| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Not applicable; this is a fluorescent pH indicator dye. It targets proton/hydroxyl ions via its pH-dependent ionization state. The dye is used for imaging intracellular pH changes rather than binding to a specific biological target like a receptor or enzyme.
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|---|---|
| ln Vitro |
5(6)-Carboxynaphthalene fluorescein, having a pKa value of 7.6, is a luminous pH indicator that is sensitive in the pH range of 6.6 to 8.6 [1]. The emission spectrum of 5-(6)-carboxynaphthalene fluorescein is 668 nm, and its excitation wavelength is 593 nm [1].
The compound serves as a ratiometric pH indicator with a pKa of 7.6, functioning effectively over the pH range of 6.6 to 8.6. It exhibits intense red fluorescence at basic pH and shifts to shorter wavelengths (green fluorescence) at acidic or neutral pH, enabling quantification of proton concentration changes. |
| ln Vivo |
Not applicable; this is an in vitro imaging probe and is not administered as a therapeutic agent in animal models. It can be used in ex vivo assays on tissue sections or in live-cell imaging to monitor intracellular pH during pharmacological treatments.
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| Enzyme Assay |
A working solution is prepared at 1-10 mM in DMSO. For assay, it is diluted to 1-10 microM in pH calibration buffers (e.g., pH 4.0-9.0, 50 mM citrate or phosphate buffer). Fluorescence is measured using a spectrophotometer (λex=512 nm, λem=567 nm) or a plate reader to generate a pH standard curve.
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| Cell Assay |
Cells are loaded with 5(6)-Carboxynaphthofluorescein by incubation with 1-10 microM of the dye in culture medium for 15-30 minutes at 37degC. After washing, cells are treated with test compounds or pH-altering agents, and fluorescence is analyzed by flow cytometry (FL1 channel) or confocal microscopy.
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| Animal Protocol |
Not applicable; this dye is not administered to animals in standard protocols. However, it can be loaded into cells ex vivo after they are harvested from treated animals, allowing researchers to study pH changes in primary cells derived from various disease models.
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| ADME/Pharmacokinetics |
Not applicable for the dye itself. The compound is stable in aqueous solutions for several hours but gradually photobleaches under prolonged illumination. Its cell-permeant properties allow rapid equilibration across cell membranes, with intracellular accumulation reaching a steady state within 30 minutes.
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| Toxicity/Toxicokinetics |
No toxicity; the compound is used at low micromolar concentrations for cell labeling and exhibits minimal cytotoxicity in short-term experiments. Standard laboratory safety precautions (gloves, lab coat, eye protection) should be followed when handling the solid dye.
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| References | |
| Additional Infomation |
5(6)-Carboxynaphthofluorescein is for research use only, not FDA-approved. It is widely used for monitoring endosomal/lysosomal acidification, evaluating drug effects on cellular pH homeostasis, and developing pH-sensitive biosensors for wound monitoring and other biomedical applications.
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| Molecular Formula |
2[C29H16O7]
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|---|---|
| Molecular Weight |
952.866280000001
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| Exact Mass |
476.09
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| CAS # |
128724-35-6
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| Appearance |
Brown to black solid powder
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| LogP |
6.021
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| InChi Key |
SHYSWDIJAFDQED-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C28H18O3/c1-16-3-2-4-19(13-16)26-24-9-5-17-14-20(29)7-11-22(17)27(24)31-28-23-12-8-21(30)15-18(23)6-10-25(26)28/h2-15,29H,1H3
|
| Chemical Name |
19-hydroxy-13-(3-methylphenyl)-2-oxapentacyclo[12.8.0.03,12.04,9.017,22]docosa-1(14),3,5,8,10,12,15,17(22),18,20-decaen-7-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~262.36 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0495 mL | 5.2473 mL | 10.4946 mL | |
| 5 mM | 0.2099 mL | 1.0495 mL | 2.0989 mL | |
| 10 mM | 0.1049 mL | 0.5247 mL | 1.0495 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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