| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
The primary target of CDF is the MRP2 (multidrug resistance-associated protein 2) transporter, as it is a substrate for this efflux pump. Its mechanism of action in this context involves being transported by MRP2 across membranes. As a fluorescent probe, its mechanism is based on its fluorescence properties. Upon oxidation by reactive oxygen species, the non-fluorescent dihydro form is converted to the highly fluorescent fluorescein form, allowing for the detection of ROS. This property makes it a valuable tool for assessing oxidative stress in cells.
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| ln Vitro |
In vitro, CDF is used as a fluorescein that is an ideal substrate for MRP2 vesicle transport experiments. It is also used as a fluorescent probe for detecting reactive oxygen species (ROS) and assessing cellular health. The compound's ability to be transported by MRP2 makes it a useful tool for studying the function of this efflux pump. Its fluorescence properties allow for sensitive detection of ROS in cell-based assays.
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| ln Vivo |
In vivo activity of CDF is not typically studied. However, as a substrate for MRP2, it could potentially be used to assess MRP2 function in vivo. Its use as a ROS probe could also be applied in vivo to measure oxidative stress in tissues. However, specific in vivo data is not available in the literature.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays using CDF typically involve the compound as a substrate for MRP2 or as a probe for ROS. A standard protocol for MRP2 vesicle transport assay: membrane vesicles containing MRP2 are incubated with CDF in a buffer containing ATP. The uptake of CDF into the vesicles is measured by fluorescence. The compound's transport by MRP2 is assessed by comparing the uptake in the presence and absence of ATP or in the presence of MRP2 inhibitors.
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| Cell Assay |
In vitro cell-based assays using CDF typically involve measuring ROS production or MRP2 activity. A standard protocol for ROS detection: cells are loaded with CDF (e.g., by incubation with the acetoxymethyl ester form) for 30-60 minutes at 37°C. The cells are then washed and stimulated with an agent that induces ROS production (e.g., hydrogen peroxide). The increase in fluorescence is measured over time using a fluorescence plate reader or flow cytometry. For MRP2 activity, cells expressing MRP2 are incubated with CDF, and the efflux of the compound is measured.
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| Animal Protocol |
In vivo animal experiments for CDF are not well-documented. For ROS detection, CDF could potentially be used to measure oxidative stress in tissues. For example, the compound could be injected into animals, and its fluorescence in various tissues could be measured. However, specific protocols and data are not available.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of CDF are not a primary focus of study. The compound is a fluorescent probe used in cell-based assays. Its cell permeability depends on the form used (e.g., the acetoxymethyl ester form is cell-permeable). The compound is typically stored as a powder and protected from light.
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| Toxicity/Toxicokinetics |
Toxicity data for CDF is limited. As a fluorescent probe, it is generally considered to have low toxicity at the concentrations used for assays. However, standard laboratory safety precautions should be followed when handling the compound. The compound is not intended for human use.
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| References | |
| Additional Infomation |
5-Carboxy-2',7'-Dichlorofluorescein is an oxaspirocyclic compound.
CDF is a fluorescent compound used as a probe for ROS and as a substrate for MRP2 vesicle transport experiments. It is also known as 5(6)-Carboxy-2',7'-dichlorofluorescein. It is not a drug and has no clinical use or approval status. It is commercially available from chemical suppliers for research purposes only. |
| Molecular Formula |
C21H10O7CL2
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|---|---|
| Molecular Weight |
445.2059
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| Exact Mass |
443.98
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| CAS # |
111843-78-8
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| PubChem CID |
132525
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| Appearance |
Yellow to orange solid powder
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| Density |
1.86g/cm3
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| Boiling Point |
732.3ºC at 760mmHg
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| Melting Point |
250ºC
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| Flash Point |
396.7ºC
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| Vapour Pressure |
0mmHg at 25°C
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| LogP |
4.67
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
30
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| Complexity |
702
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JGZVUTYDEVUNMK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H10Cl2O7/c22-13-4-11-17(6-15(13)24)29-18-7-16(25)14(23)5-12(18)21(11)10-2-1-8(19(26)27)3-9(10)20(28)30-21/h1-7,24-25H,(H,26,27)
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| Chemical Name |
2',7'-dichloro-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~31.25 mg/mL (~70.19 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2461 mL | 11.2307 mL | 22.4613 mL | |
| 5 mM | 0.4492 mL | 2.2461 mL | 4.4923 mL | |
| 10 mM | 0.2246 mL | 1.1231 mL | 2.2461 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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