| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate does not have a specific biological target in the traditional pharmacological sense. Its mechanism of action is chemical and relates to its use as a fluorogenic probe. The compound is a non-fluorescent, membrane-permeable diacetate ester that is hydrolyzed by intracellular esterases to release the fluorescent carboxy-2',7'-dichlorofluorescein (CDF) product. The fluorescence of CDF is dependent on intracellular esterase activity and can also be used to measure intracellular pH due to the pH-sensitivity of CDF fluorescence.
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| ln Vitro |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is non-fluorescent in its intact form. Upon hydrolysis by intracellular esterases, it is converted to the fluorescent carboxy-2',7'-dichlorofluorescein (CDF), which has excitation and emission maxima at approximately 495 nm and 520 nm, respectively. The fluorescence intensity is proportional to the esterase activity in the cell and can also be used as an indicator of cell viability. The compound does not exhibit pharmacological activity; its utility is as a fluorogenic probe for cell biology research.
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| ln Vivo |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate does not have in vivo pharmacological activity. It is a fluorogenic probe used for research applications, primarily in vitro and ex vivo, to assess cell viability, esterase activity, and phagocytosis. The compound may be used in flow cytometry or fluorescence microscopy applications with cells isolated from animal models, but this is for research purposes and not for therapeutic evaluation.
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| Enzyme Assay |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is used as a fluorogenic probe in non-cellular assays to measure esterase activity. In a typical assay, the compound is dissolved in an appropriate buffer, and esterase enzyme (e.g., from porcine liver) is added. The hydrolysis of the compound is monitored by measuring the increase in fluorescence over time using a spectrofluorometer or fluorescence microplate reader with excitation at 495 nm and emission at 520 nm.
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| Cell Assay |
In vitro cellular assays for 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate typically involve loading cells with the probe to assess esterase activity, cell viability, or phagocytosis. Cells are incubated with the compound at concentrations of 1-10 μM for 15-60 minutes. The non-fluorescent diacetate ester enters cells and is hydrolyzed by intracellular esterases to the fluorescent CDF product. Fluorescence is measured using flow cytometry or fluorescence microscopy. For phagocytosis assays, fluorescently labeled particles are used in combination with CDFDA to distinguish phagocytic cells from non-phagocytic cells.
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| Animal Protocol |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is not used in in vivo animal studies as a therapeutic or pharmacological agent. Its application is limited to ex vivo analysis of cells collected from animals. For example, cells isolated from blood, tissues, or tumors can be stained with CDFDA and analyzed by flow cytometry to assess cell viability, esterase activity, or phagocytic function.
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| ADME/Pharmacokinetics |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is not a drug and does not have pharmacokinetic properties. Its physical and chemical properties are more relevant: it is a fluorogenic probe with a molecular weight of 529.28 and a molecular formula of C₂₅H₁₄Cl₂O₉. The compound is soluble in DMSO and is typically prepared as a stock solution in DMSO for addition to aqueous buffers or cell culture media.
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| Toxicity/Toxicokinetics |
Specific toxicological data for 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate are not extensively detailed in standard product information. As a research chemical, it should be handled with standard laboratory precautions, avoiding inhalation, ingestion, and skin contact. The compound is not intended for human use. CDFDA is generally considered to have low toxicity at the concentrations used for cell staining (typically 1-10 μM).
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| References | |
| Additional Infomation |
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate is a research-grade fluorogenic probe intended for laboratory use only and is not approved for clinical use. Its CAS number is 127770-45-0. The primary applications of 5(6)-CDFDA include assessment of cell viability and esterase activity in flow cytometry and fluorescence microscopy, measurement of intracellular pH, and detection of phagocytosis. The compound is a valuable tool for cell biology, immunology, and drug discovery research.
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| Molecular Formula |
C25H14CL2O9
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|---|---|
| Molecular Weight |
529.27900
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| Exact Mass |
528.001
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| CAS # |
127770-45-0
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| PubChem CID |
16211578
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
743.3±60.0 °C at 760 mmHg
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| Melting Point |
210ºC (dec.)(lit.)
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| Flash Point |
403.3±32.9 °C
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| Vapour Pressure |
0.0±2.6 mmHg at 25°C
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| Index of Refraction |
1.712
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| LogP |
3.66
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
18
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
72
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| Complexity |
1790
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8894 mL | 9.4468 mL | 18.8936 mL | |
| 5 mM | 0.3779 mL | 1.8894 mL | 3.7787 mL | |
| 10 mM | 0.1889 mL | 0.9447 mL | 1.8894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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