| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 500mg | |||
| Other Sizes |
| Targets |
4-Di-16-ASP targets cell membranes, specifically the lipid bilayer. It is a lipophilic dye that integrates into lipid bilayers and labels plasma membranes. It is used for membrane staining and tracking neuronal activity. The dye's hydrophobic structure supports selective staining of neuronal processes, including axons and dendrites, without crossing into the cytoplasm.
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|---|---|
| ln Vitro |
In vitro, 4-Di-16-ASP is used as a fluorescent membrane dye. It is a lipophilic aminostyryl probe with a broad emission spectrum. It is widely applied in neuroscience, cell biology, and live-cell imaging, offering long-term retention and high photostability. It is used for labeling plasma membranes and monitoring membrane potential changes.
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| ln Vivo |
There are no significant in vivo applications for 4-Di-16-ASP as a therapeutic agent. It is a fluorescent dye for in vitro and ex vivo imaging. It is not administered to live animals for therapeutic purposes.
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| Enzyme Assay |
There are no in vitro enzyme/receptor binding assays for 4-Di-16-ASP as it is a fluorescent dye, not an enzyme inhibitor or receptor ligand.
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| Cell Assay |
In vitro cell-based assays for 4-Di-16-ASP involve incubating cells with the dye, which partitions into the plasma membrane. Fluorescence microscopy or flow cytometry is used to visualize or quantify membrane staining. The dye is used to study membrane dynamics, neuronal activity, and cell morphology.
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| Animal Protocol |
There are no standard in vivo animal experimental protocols for 4-Di-16-ASP as it is not administered to live animals for therapeutic or pharmacokinetic studies. It is a research tool for in vitro cell biology imaging.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 4-Di-16-ASP are not characterized as it is not a drug. It is a fluorescent dye for research use. It is lipophilic and integrates into lipid bilayers. For storage, it should be kept at -20°C.
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| Toxicity/Toxicokinetics |
No toxicity data for 4-Di-16-ASP are available. As a research chemical, it should be handled with standard laboratory safety precautions. It is not for human or veterinary use.
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| References | |
| Additional Infomation |
4-(4-Hexadecylaminostyryl)-N-methylpyridine iodide is an organic iodide salt and a pyridine salt. It is used as a fluorescent dye. It contains 4-(4-hexadecylaminostyryl)-N-methylpyridine.
4-Di-16-ASP is a green fluorescent membrane dye. It is a lipophilic aminostyryl probe with a broad emission spectrum. It is used for membrane staining and neuronal activity tracking. Its CAS number is 114041-00-8. |
| Molecular Formula |
C46H79N2+.I-
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|---|---|
| Molecular Weight |
787.03686
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| Exact Mass |
786.529
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| CAS # |
114041-00-8
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| PubChem CID |
6439243
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| Appearance |
Brown to reddish brown solid powder
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| LogP |
11.454
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
33
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| Heavy Atom Count |
49
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| Complexity |
640
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCCCN(CCCCCCCCCCCCCCCC)C1=CC=C(C=C1)/C=C/C2=CC=[N+](C=C2)C.[I-]
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| InChi Key |
YPGZWUVVEWKKDQ-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C46H79N2.HI/c1-4-6-8-10-12-14-16-18-20-22-24-26-28-30-40-48(41-31-29-27-25-23-21-19-17-15-13-11-9-7-5-2)46-36-34-44(35-37-46)32-33-45-38-42-47(3)43-39-45;/h32-39,42-43H,4-31,40-41H2,1-3H3;1H/q+1;/p-1
|
| Chemical Name |
N,N-dihexadecyl-4-[(E)-2-(1-methylpyridin-1-ium-4-yl)ethenyl]aniline;iodide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~2 mg/mL (~2.54 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2706 mL | 6.3529 mL | 12.7058 mL | |
| 5 mM | 0.2541 mL | 1.2706 mL | 2.5412 mL | |
| 10 mM | 0.1271 mL | 0.6353 mL | 1.2706 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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