| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
4-Bromomethyl-6,7-dimethoxycoumarin does not have a specific biological target as a drug. It is a fluorescent labeling reagent used in analytical chemistry. The compound contains a bromomethyl group that reacts with carboxyl groups to form the corresponding ester in the presence of a base. This reaction is used to label carboxylic acids for detection by HPLC with fluorescence detection. Its value is in analytical chemistry and organic synthesis.
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| ln Vitro |
4-Bromomethyl-6,7-dimethoxycoumarin does not have direct in vitro biological activity as a pharmacological agent. Its value is as a fluorescent labeling reagent for the detection of carboxylic acids in chromatographic analysis. The compound is used in HPLC labeling and detection applications.
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| ln Vivo |
4-Bromomethyl-6,7-dimethoxycoumarin does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for analytical chemistry.
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| Enzyme Assay |
In vitro analytical assays using 4-Bromomethyl-6,7-dimethoxycoumarin involve its use as a fluorescent labeling reagent for carboxylic acids. The compound reacts with carboxylic acids in the presence of a base to form fluorescent esters. These derivatives are then separated by reversed-phase HPLC and detected by fluorescence.
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| Cell Assay |
Cell culture experiments are not applicable to 4-Bromomethyl-6,7-dimethoxycoumarin as it is a labeling reagent for analytical chemistry, not a pharmacological compound. The compound is used in chemical analysis and is not intended for cell-based assays.
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| Animal Protocol |
In vivo animal experiments are not applicable to 4-Bromomethyl-6,7-dimethoxycoumarin as it is a labeling reagent, not a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for analytical chemistry.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to 4-Bromomethyl-6,7-dimethoxycoumarin as it is a labeling reagent, not a therapeutic agent. The compound has a molecular formula of C12H11BrO4 and a molecular weight of 299.12. Appearance: Light yellow to amber to dark green powder to crystal. Storage: room temperature.
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| Toxicity/Toxicokinetics |
Safety and toxicology data for 4-Bromomethyl-6,7-dimethoxycoumarin are limited. As a chemical reagent, it should be handled with appropriate safety precautions. The compound is for research use only and is not for human therapeutic use. No specific toxicity data are available.
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| References |
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| Additional Infomation |
4-Bromomethyl-6,7-dimethoxycoumarin has CAS number 88404-25-5, molecular formula C12H11BrO4, and molecular weight 299.12. Synonyms: 4-BDMC. It is a fluorescent labeling reagent for carboxylic acids used in HPLC detection. Purity: ≥95%. Not for human use; for research purposes only.
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| Molecular Formula |
C12H11BRO4
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|---|---|
| Molecular Weight |
299.1173
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| Exact Mass |
297.984
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| CAS # |
88404-25-5
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| PubChem CID |
128870
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
417.1±45.0 °C at 760 mmHg
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| Melting Point |
212-216 °C(lit.)
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| Flash Point |
206.0±28.7 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.579
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| LogP |
2.7
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
17
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| Complexity |
329
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
JGODLBJJCNQFII-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H11BrO4/c1-15-10-4-8-7(6-13)3-12(14)17-9(8)5-11(10)16-2/h3-5H,6H2,1-2H3
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| Chemical Name |
4-(bromomethyl)-6,7-dimethoxychromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~10 mg/mL (~33.43 mM ()
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3431 mL | 16.7157 mL | 33.4314 mL | |
| 5 mM | 0.6686 mL | 3.3431 mL | 6.6863 mL | |
| 10 mM | 0.3343 mL | 1.6716 mL | 3.3431 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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