| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
The compound itself is not a drug that targets a biological receptor; it is a chemical intermediate for dye synthesis. The dyes prepared from this building block may target various biological molecules, such as proteins and nucleic acids, for fluorescent labeling and imaging. The anhydride functional group allows for conjugation with amines, enabling the attachment of fluorescent labels to biomolecules. The sulfonate groups enhance water solubility, which is important for biological applications.
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| ln Vitro |
In vitro, the dyes synthesized from this building block are used for fluorescent labeling of cells and biomolecules. The compound itself is not biologically active but serves as a precursor for active fluorescent probes. The resulting dyes can be used in various in vitro assays, including fluorescence microscopy, flow cytometry, and immunohistochemistry. The sulfonate groups provide water solubility, and the anhydride group allows for conjugation to amine-containing molecules.
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| ln Vivo |
In vivo, dyes synthesized from this building block may be used for imaging applications, such as tracking cells or detecting specific biomarkers in live animals. The water-soluble nature of the dyes, conferred by the sulfonate groups, makes them suitable for intravenous administration. However, the specific in vivo applications depend on the properties of the final dye product. The compound itself is not administered in vivo but is used as a synthetic intermediate.
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| Enzyme Assay |
The compound is used as a starting material for the synthesis of fluorescent dyes. The anhydride functional group reacts with primary amines to form amide bonds, allowing the attachment of the dye to biomolecules. The reaction is typically carried out in a suitable solvent, such as DMSO or an aqueous buffer, under mild conditions. The resulting dye conjugates can be purified by chromatography and characterized by mass spectrometry and fluorescence spectroscopy. The protocols are for research purposes only and have not been independently validated by all suppliers.
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| Animal Protocol |
The compound is not used directly in animal experiments but is used to synthesize dyes for in vivo imaging studies. The dyes are typically administered intravenously to animal models to visualize specific tissues or cells. The animals are then imaged using appropriate fluorescence imaging systems. The protocols depend on the specific dye and the biological target. The compound is for research use only and not for human therapeutic use.
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| ADME/Pharmacokinetics |
4-Amino-3,6-disulfonaphthalic anhydride dipotassium is a water-soluble building block with a molecular weight of 449.50. It contains sulfonate groups that enhance water solubility and an anhydride group that reacts with amines. The compound is a solid and should be stored at room temperature in a dry environment. It is stable under standard laboratory conditions. The compound is for research use only and not for human therapeutic use.
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| Toxicity/Toxicokinetics |
Specific toxicity data for 4-Amino-3,6-disulfonaphthalic anhydride dipotassium are not extensively documented. As a chemical intermediate, it may be irritating to the skin, eyes, and respiratory tract. The compound is for research use only and not for human therapeutic use. It should be handled with appropriate safety precautions, including the use of personal protective equipment. The compound should be stored in a sealed container in a cool, dry place away from incompatible materials.
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| References | |
| Additional Infomation |
4-Amino-3,6-disulfonaphthalic anhydride dipotassium is also known as Lucifer Yellow anhydride dipotassium salt. It is a molecular building block used to prepare dyes for biological research. It is a dipotassium salt with the molecular formula C₁₂H₅K₂NO₈S₂. The compound is used as an intermediate in the synthesis of fluorescent probes for imaging and labeling applications. It is supplied as a solid and should be stored at room temperature. It is for research use only.
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| Molecular Formula |
C12H5NO9S2-2.2[K+]
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|---|---|
| Molecular Weight |
449.496
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| Exact Mass |
448.868
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| CAS # |
79539-35-8
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| PubChem CID |
12868665
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| Appearance |
Typically exists as solid at room temperature
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| Melting Point |
>300ºC(lit.)
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| LogP |
2.283
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
26
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| Complexity |
752
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[K].O=C1C2C3C(C=C(S(O)(=O)=O)C=2)=C(N)C(S(O)(=O)=O)=CC=3C(=O)O1
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| InChi Key |
MIOVHACHHDENNE-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C12H7NO9S2.2K/c13-10-5-1-4(23(16,17)18)2-6-9(5)7(12(15)22-11(6)14)3-8(10)24(19,20)21;;/h1-3H,13H2,(H,16,17,18)(H,19,20,21);;/q;2*+1/p-2
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| Chemical Name |
dipotassium;8-amino-2,4-dioxo-3-oxatricyclo[7.3.1.05,13]trideca-1(12),5,7,9(13),10-pentaene-7,11-disulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2247 mL | 11.1235 mL | 22.2469 mL | |
| 5 mM | 0.4449 mL | 2.2247 mL | 4.4494 mL | |
| 10 mM | 0.2225 mL | 1.1123 mL | 2.2247 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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