| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary targets of 4'-Hydroxy-2,4-dimethoxychalcone include oxidative stress pathways, inflammatory mediators, and cancer-related signaling pathways. It exhibits antioxidant activity by scavenging free radicals and modulating antioxidant enzyme activities. The compound also inhibits the production of pro-inflammatory cytokines and suppresses the activation of NF-κB. In cancer cells, it induces apoptosis and inhibits cell proliferation through various mechanisms, including modulation of cell cycle regulators and induction of apoptosis.
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| ln Vitro |
4'-Hydroxy-2,4-dimethoxychalcone (compound 2) is antagonistic to P. falciparum W2 (IC50=4.8 μM) and D6 (IC50=4.2 μM)[1].
In vitro, 4'-Hydroxy-2,4-dimethoxychalcone demonstrates potent antioxidant activity in DPPH and ABTS assays. It shows anti-inflammatory effects by reducing NO production and TNF-α secretion in LPS-stimulated macrophages. The compound inhibits the growth of various cancer cell lines, including breast, colon, and lung cancer cells, with IC50 values typically in the micromolar range. It also exhibits antimicrobial activity against certain bacterial and fungal strains. |
| ln Vivo |
In four- to five-week-old ICR mice, 4'-hydroxy-2,4-dimethoxychalcone (compound 2; oral; 160 mg/kg) exhibits a T1/2 of 1.94 hours, a Cmax of 158 ng/mL, and an AUC of 384 ng·h/mL[1].
In vivo activity of 4'-Hydroxy-2,4-dimethoxychalcone has been reported in some studies, showing anti-inflammatory effects in carrageenan-induced paw edema models and antitumor activity in xenograft models. However, comprehensive pharmacokinetic and pharmacodynamic data are limited. The compound is primarily studied in vitro, and further in vivo investigations are needed to confirm its therapeutic potential. |
| Enzyme Assay |
In vitro enzyme/receptor binding assays for 4'-Hydroxy-2,4-dimethoxychalcone typically involve antioxidant assays (DPPH, ABTS, FRAP), anti-inflammatory assays (measuring NO, PGE2, and cytokines in macrophages), and enzyme inhibition assays (e.g., COX, LOX). The compound is tested in a concentration range of 0.1-100 µM.
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| Cell Assay |
In vitro cellular assays for 4'-Hydroxy-2,4-dimethoxychalcone involve testing its effects on cell viability, apoptosis, and inflammatory markers. Cancer cell lines are treated with serial dilutions of the compound, and cell viability is assessed using MTT or SRB assays. Apoptosis is evaluated by Annexin V/PI staining and caspase activity. Inflammatory markers are measured in macrophage cultures using ELISA. The compound shows concentration-dependent activities.
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| Animal Protocol |
In vivo animal studies for 4'-Hydroxy-2,4-dimethoxychalcone are limited. For anti-inflammatory testing, carrageenan-induced paw edema in rats is a common model, with oral or intraperitoneal administration of the compound at doses of 10-50 mg/kg. For antitumor testing, xenograft models in mice are used, with daily treatment and monitoring of tumor volume. Detailed protocols are not widely reported.
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| ADME/Pharmacokinetics |
4'-Hydroxy-2,4-dimethoxychalcone has a molecular formula of C17H16O4 and a molecular weight of 284.31. It appears as a solid powder with a purity of ≥98%. It is soluble in DMSO and ethanol, but sparingly soluble in water. The compound should be stored at -20°C, protected from light, and is stable for up to 2 years under dry conditions. It is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of 4'-Hydroxy-2,4-dimethoxychalcone has not been extensively characterized. As a chalcone derivative, it is generally considered to have moderate toxicity. Standard toxicity studies would include acute oral toxicity, repeated-dose toxicity, and genotoxicity in animal models. The compound is intended for research use only and is not approved for clinical use.
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| References | |
| Additional Infomation |
Reports indicate that dragon's blood tree contains (E)-3-(2,4-dimethoxyphenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one, and there is related data.
4'-Hydroxy-2,4-dimethoxychalcone (CAS 151135-64-7) is a chalcone derivative found in Glycyrrhiza species. It has a molecular formula of C17H16O4 and a molecular weight of 284.31. The compound exhibits antioxidant, anti-inflammatory, anticancer, and antimicrobial activities. It scavenges free radicals, inhibits pro-inflammatory mediators, induces apoptosis in cancer cells, and shows antimicrobial effects. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C17H16O4
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|---|---|
| Molecular Weight |
284.31
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| Exact Mass |
284.104
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| CAS # |
151135-64-7
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| PubChem CID |
5729232
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
500.1±50.0 °C at 760 mmHg
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| Flash Point |
185.8±23.6 °C
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| Vapour Pressure |
0.0±1.3 mmHg at 25°C
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| Index of Refraction |
1.614
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| LogP |
3.48
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
21
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| Complexity |
358
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=CC(=C(C=C1)/C=C/C(=O)C2=CC=C(C=C2)O)OC
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| InChi Key |
LNEYYODFMSUKGY-UXBLZVDNSA-N
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| InChi Code |
InChI=1S/C17H16O4/c1-20-15-9-5-13(17(11-15)21-2)6-10-16(19)12-3-7-14(18)8-4-12/h3-11,18H,1-2H3/b10-6+
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| Chemical Name |
(E)-3-(2,4-dimethoxyphenyl)-1-(4-hydroxyphenyl)prop-2-en-1-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL | |
| 5 mM | 0.7035 mL | 3.5173 mL | 7.0346 mL | |
| 10 mM | 0.3517 mL | 1.7586 mL | 3.5173 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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