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| 50mg |
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| 100mg |
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| Targets |
4,4'-Iminodiphenol has been identified as an inactive estrogen receptor ligand. It does not activate the estrogen receptor, making it useful as a negative control in studies investigating estrogen receptor signaling. Its primary utility is in materials science as a building block for polymers, where it contributes to the structural properties of the final material rather than interacting with biological targets.
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| ln Vitro |
4,4'-Iminodiphenol is characterized as an inactive estrogen receptor ligand, meaning it shows no significant agonistic or antagonistic activity at the estrogen receptor in standard in vitro assays. Its biological inactivity in this context makes it a valuable negative control for experiments investigating estrogen receptor signaling. In materials science, its properties as a difunctional phenol allow it to act as a monomer in polymerization reactions.
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| ln Vivo |
In vivo activity data for 4,4'-Iminodiphenol are not extensively reported, as its primary applications are in materials science and as an in vitro research tool. As an inactive estrogen receptor ligand, it is not expected to elicit significant in vivo hormonal effects. Its use in biological research is largely confined to in vitro assays where it serves as a control compound to validate the specificity of estrogen receptor-mediated responses.
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| Enzyme Assay |
In vitro enzyme/receptor binding (non-cell) assays for 4,4'-Iminodiphenol typically involve radioligand binding studies to evaluate its interaction with the estrogen receptor. The receptor is incubated with increasing concentrations of the compound and a fixed concentration of a radiolabeled estrogen (e.g., ³H-estradiol) in binding buffer. Bound and free radioligand are separated, and radioactivity is measured. The compound's lack of significant binding or activity is confirmed, establishing it as an inactive ligand.
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| Cell Assay |
For in vitro cell-based assays, cells expressing the estrogen receptor are cultured and treated with 4,4'-Iminodiphenol at various concentrations. Its inactivity is confirmed by measuring the expression of estrogen-responsive genes via qRT-PCR or by assessing cell proliferation. The compound shows no significant effect, validating its use as a negative control in studies of estrogen receptor signaling. Cell viability can be assessed using standard assays like MTT.
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| Animal Protocol |
In vivo animal studies with 4,4'-Iminodiphenol are not typically performed, as it is not a therapeutic candidate. Its use is confined to in vitro research as a negative control for estrogen receptor studies and as a building block in polymer chemistry. The compound is not intended for in vivo pharmacological evaluation, and no specific animal study protocols are available in the literature.
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| ADME/Pharmacokinetics |
4,4'-Iminodiphenol (CAS: 1752-24-5) has a molecular weight of 201.22 g/mol and a molecular formula of C12H11NO2. It is also known as 4-(4-hydroxyanilino)phenol or leucoindophenol. As a solid compound, it is typically stored under standard laboratory conditions. Its physicochemical properties are consistent with its use as a monomer in polymer synthesis and as a research chemical.
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| Toxicity/Toxicokinetics |
4,4'-Iminodiphenol is considered to have a low toxicity profile, consistent with its use as a chemical intermediate and research compound. It is not intended for human therapeutic use. As an inactive estrogen receptor ligand, it does not pose hormonal risks. Standard laboratory safety precautions should be followed when handling the compound. No significant toxicity has been reported in the available literature.
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| Additional Infomation |
4,4'-Iminodiphenol is an organic compound with the CAS number 1752-24-5, also known as 4,4'-dihydroxydiphenylamine. It is a key intermediate in the synthesis of polymers and resins. In biological research, it serves as an inactive estrogen receptor ligand and a useful negative control for studies of estrogen signaling. It is not an approved drug and is intended for research and industrial use only.
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| Molecular Formula |
C12H11NO2
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| Molecular Weight |
201.22124
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| Exact Mass |
201.079
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| CAS # |
1752-24-5
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| PubChem CID |
74461
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.32g/cm3
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| Boiling Point |
363ºC at 760mmHg
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| Flash Point |
158.8ºC
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| Vapour Pressure |
8.88E-06mmHg at 25°C
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| Index of Refraction |
1.707
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| LogP |
2.914
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
15
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| Complexity |
162
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| Defined Atom Stereocenter Count |
0
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| SMILES |
N(C1C=CC(O)=CC=1)C1C=CC(O)=CC=1
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| InChi Key |
YRUPBAWWCPVHFT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H11NO2/c14-11-5-1-9(2-6-11)13-10-3-7-12(15)8-4-10/h1-8,13-15H
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| Chemical Name |
4-(4-hydroxyanilino)phenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9697 mL | 24.8484 mL | 49.6968 mL | |
| 5 mM | 0.9939 mL | 4.9697 mL | 9.9394 mL | |
| 10 mM | 0.4970 mL | 2.4848 mL | 4.9697 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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