| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
DIB-Cl targets primary and secondary amine groups on small molecules, including excitatory neurotransmitters (e.g., glutamate, aspartate) and other amine-containing compounds. It is a chemical derivatization reagent, not a biological target-directed drug.
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|---|---|
| ln Vitro |
In cell-free derivatization reactions, DIB-Cl reacts with amines to form stable fluorescent amide derivatives. The resulting conjugates are highly fluorescent and can be detected with high sensitivity (low picomole to femtomole levels) via HPLC with fluorescence detection. The reagent shows high reactivity and specificity for amines under mild reaction conditions.
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| ln Vivo |
No biological activity data are documented as DIB-Cl is a derivatization reagent for analytical chemistry, not a pharmacologically active compound for in vivo or cell-based assays.
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| Enzyme Assay |
This is a chemical derivatization reaction, not a biological enzyme or receptor binding assay. Amine-containing compounds (primary or secondary amines) are dissolved in an appropriate organic solvent or aqueous buffer (pH 8-9). DIB-Cl is added in molar excess (2-10 equivalents) and the reaction proceeds at room temperature or 40-60degC for 15-60 min with occasional shaking. The resulting fluorescent amide derivatives are analyzed by reversed-phase HPLC with fluorescence detection (Ex/Em ~250-350/400-500 nm).
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| Cell Assay |
No cell-based assays are performed with DIB-Cl as it is a chemical derivatization reagent, not a live-cell probe. Instead, cells or tissues are processed to extract amine-containing analytes of interest. The extracted amines are then derivatized with DIB-Cl as described in the cell-free protocol, and the derivatives are analyzed by HPLC-FLD to quantify amine levels in the original biological sample.
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| Animal Protocol |
DIB-Cl is not administered to live animals as it is a derivatization reagent. For tissue amine analysis, animals are euthanized, and brain or other tissues are rapidly dissected, homogenized, and deproteinized. Amines are extracted, then derivatized with DIB-Cl under optimal reaction conditions (pH 8-9, 40-60degC, 15-60 min). Fluorescent derivatives are analyzed by HPLC-FLD to quantify excitatory amino acids and other amines in brain tissue.
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| ADME/Pharmacokinetics |
Pharmacokinetic data are not applicable as DIB-Cl is a derivatization reagent for analytical chemistry, not a drug candidate. The reagent is stable as a solid when stored at -20degC, protected from moisture and light. For use, a stock solution is prepared in anhydrous organic solvent (e.g., acetonitrile, DMF, DMSO) at 1-10 mM immediately before use, as the compound may be moisture-sensitive.
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| Toxicity/Toxicokinetics |
DIB-Cl is a reactive acyl chloride and potential lachrymator. It can cause irritation to the eyes, respiratory tract, and skin. Standard safety precautions for reactive acyl chlorides should be strictly followed: use only in a well-ventilated fume hood, avoid inhalation and skin contact, wear appropriate PPE (gloves, lab coat, safety goggles), and ensure all containers are tightly sealed to prevent exposure to moisture. The compound is for research use only, not for human consumption.
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| References | |
| Additional Infomation |
This is a research-use-only fluorescent labeling reagent, not a drug. It has been successfully used in HPLC determination of excitatory amines in biological samples. Molecular formula: C22H15ClN2O; molecular weight: 358.82. Purity: ≥98%. Also known as DIB-Cl and 4-(4,5-Diphenyl-imidazol-2-yl)benzoyl-chloride. Store at -20degC in a sealed, dry container, protected from light.
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| Molecular Formula |
C22H15N2OCL
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|---|---|
| Molecular Weight |
358.8203
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| Exact Mass |
358.087
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| CAS # |
162756-62-9
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| PubChem CID |
4072355
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| Appearance |
White to off-white solid powder
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| LogP |
5.789
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
26
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| Complexity |
465
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PHMRAWURTKPWFB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H15ClN2O/c23-21(26)17-11-13-18(14-12-17)22-24-19(15-7-3-1-4-8-15)20(25-22)16-9-5-2-6-10-16/h1-14H,(H,24,25)
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| Chemical Name |
4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~33.33 mg/mL (~92.89 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7869 mL | 13.9346 mL | 27.8691 mL | |
| 5 mM | 0.5574 mL | 2.7869 mL | 5.5738 mL | |
| 10 mM | 0.2787 mL | 1.3935 mL | 2.7869 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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