| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| ln Vitro |
3-O-Acetyl-16α-hydroxytrametenolic acid showed cytotoxic activity against human lung carcinoma A549 cells with an IC50 value of 44.3 ± 39.1 µM, and against human prostate carcinoma DU145 cells with an IC50 value of 140 ± 50.7 µM in a 72-hour growth inhibition assay.[1]
3-O-Acetyl-16α-hydroxytrametenolic acid was tested for its antioxidant activity by measuring its ability to scavenge DPPH free radicals. At a concentration of 200 µM, the compound did not show significant free radical scavenging activity.[1] |
|---|---|
| Cell Assay |
Cell growth inhibition was quantified using the MTT assay. A549 or DU145 cells were seeded in 96-well tissue culture plates (5000 and 7500 cells per well, respectively) and cultured for 72 hours. Cells were treated with media containing the pure test compound or vehicle control in triplicate for each concentration. After treatment, MTT reagent was added to each well following the standard protocol. The absorbance of the formazan product was measured at 590 nm using a spectrophotometer. Absorbance values were normalized to vehicle controls. Data from three independent experiments were analyzed to determine IC50 values through nonlinear regression curve fitting.[1]
To evaluate antioxidant activity, a DPPH free radical scavenging assay was performed. A solution of the test compound (200 µM, 100 µL) was added to an ethanolic solution of DPPH radical (200 µM, 100 µL). The reaction mixture was shaken vigorously and incubated in the dark for 2 hours. After incubation, the absorbance of the remaining DPPH was measured at 516 nm and compared to a control containing only DPPH. Vitamin E was used as a positive control.[1] |
| References | |
| Additional Infomation |
3-O-acetyl-16α-hydroxytrimethenoic acid is a triterpenoid compound. It has been reported in the literature that 3-O-acetyl-16α-hydroxytrimethenoic acid was found in Poria cocos. 3-O-acetyl-16α-hydroxytrimethenoic acid is a known lanostane-type triterpenoid isolated from the sclerotium of Poria cocos. [1] It was isolated from a 95% ethanol extract of dried Poria cocos sclerotium by a series of chromatographic steps, including rapid silica gel column chromatography and ODS column chromatography. [1] Its structure was determined by comparing its physical and spectroscopic data (¹H-NMR, ¹³C-NMR, and MS) with those reported in the literature. [1] This study indicated that this compound, along with several other tested lanostane-type triterpenoids, exhibited better… cytotoxicity against A549 lung cancer cells compared to DU145 prostate cancer cells. [1]
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| Molecular Formula |
C32H50O5
|
|---|---|
| Molecular Weight |
514.7364
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| Exact Mass |
514.365
|
| CAS # |
168293-13-8
|
| PubChem CID |
15226712
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| Appearance |
White to off-white solid powder
|
| Density |
1.1±0.1 g/cm3
|
| Boiling Point |
606.9±55.0 °C at 760 mmHg
|
| Melting Point |
>300 °C
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| Flash Point |
185.1±25.0 °C
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| Vapour Pressure |
0.0±3.9 mmHg at 25°C
|
| Index of Refraction |
1.546
|
| LogP |
8.23
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
7
|
| Heavy Atom Count |
37
|
| Complexity |
1000
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
CC(=CCC[C@H]([C@H]1[C@@H](C[C@@]2([C@@]1(CCC3=C2CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)OC(=O)C)C)C)C)O)C(=O)O)C
|
| InChi Key |
SRDNLMOBFKJOSD-KOCSMGCBSA-N
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| InChi Code |
InChI=1S/C32H50O5/c1-19(2)10-9-11-21(28(35)36)27-24(34)18-32(8)23-12-13-25-29(4,5)26(37-20(3)33)15-16-30(25,6)22(23)14-17-31(27,32)7/h10,21,24-27,34H,9,11-18H2,1-8H3,(H,35,36)/t21-,24-,25+,26+,27+,30-,31-,32+/m1/s1
|
| Chemical Name |
(2R)-2-[(3S,5R,10S,13R,14R,16R,17R)-3-acetyloxy-16-hydroxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl]-6-methylhept-5-enoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~7.14 mg/mL (~13.87 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9427 mL | 9.7136 mL | 19.4273 mL | |
| 5 mM | 0.3885 mL | 1.9427 mL | 3.8855 mL | |
| 10 mM | 0.1943 mL | 0.9714 mL | 1.9427 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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