| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
No classical pharmacological receptor or enzyme target. The compound is a fluorescent lipid probe; its “target” is the cholesterol-rich membrane environment, where it partitions into lipid rafts and reports on cholesterol transport, esterification, and membrane organization.
|
|---|---|
| ln Vitro |
The compound itself is not a modulator of enzyme or receptor activity. Its in vitro “activity” is optical and lipid-based: it incorporates into membranes and reports on cholesterol distribution and dynamics by fluorescence microscopy, enabling studies of cholesterol uptake, efflux, and intracellular trafficking.
|
| ln Vivo |
In vivo data are limited. The probe is primarily used for in vitro live-cell imaging and for ex vivo tissue staining. Systemic administration for whole-animal imaging is not a standard application because of its lipophilicity and potential for non-specific membrane partitioning.
|
| Enzyme Assay |
For cell-free membrane studies, incorporate the probe into liposomes of defined lipid composition at 0.1–1 mol% and measure fluorescence emission (Ex ≈ 465 nm, Em ≈ 535 nm) to assess cholesterol-dependent membrane order.
|
| Cell Assay |
For live-cell imaging, incubate cells with 1–5 µM 3-NBD-C12 Cholesterol in serum-free medium for 15–60 min at 37°C. Wash and image by fluorescence microscopy using a GFP/FITC filter set. Include a no-probe control and a cholesterol-depleting agent (e.g., methyl-β-cyclodextrin) as a negative control.
|
| Animal Protocol |
For tissue staining, incubate fresh tissue sections with 1–5 µM probe in physiological buffer for 15–30 min at 37°C. Wash and image by fluorescence microscopy. Include a no-probe control and a cholesterol oxidase–treated sample to confirm specificity.
|
| References |
| Molecular Formula |
C46H72N4O5
|
|---|---|
| Molecular Weight |
761.088
|
| Exact Mass |
760.55
|
| CAS # |
186833-02-3
|
| PubChem CID |
165412500
|
| Appearance |
Yellow to orange solid powder
|
| LogP |
14.3
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
8
|
| Rotatable Bond Count |
20
|
| Heavy Atom Count |
55
|
| Complexity |
1290
|
| Defined Atom Stereocenter Count |
8
|
| SMILES |
C[C@]12CC[C@H](OC(=O)CCCCCCCCCCCN(C3=CC=C(N(=O)=O)C4=NON=C34)C)CC1=CC[C@@]1([H])[C@]3([H])CC[C@]([H])([C@H](C)CCCC(C)C)[C@@]3(C)CC[C@]21[H]
|
| InChi Key |
MKEQHMRXIADXQQ-KLHFYLHASA-N
|
| InChi Code |
InChI=1S/C46H72N4O5/c1-32(2)17-16-18-33(3)37-22-23-38-36-21-20-34-31-35(26-28-45(34,4)39(36)27-29-46(37,38)5)54-42(51)19-14-12-10-8-7-9-11-13-15-30-49(6)40-24-25-41(50(52)53)44-43(40)47-55-48-44/h20,24-25,32-33,35-39H,7-19,21-23,26-31H2,1-6H3/t33-,35+,36+,37-,38+,39+,45+,46-/m1/s1
|
| Chemical Name |
[(3S,8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl]-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] 12-[methyl-(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]dodecanoate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMF: ~10 mg/mL (~13.1 mM; with ultrasonication and warming by heating to 60°C)
DMSO: ~0.5 mg/mL (~0.7 mM; with ultrasonication and warming by heating to 60°C) |
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3139 mL | 6.5695 mL | 13.1391 mL | |
| 5 mM | 0.2628 mL | 1.3139 mL | 2.6278 mL | |
| 10 mM | 0.1314 mL | 0.6570 mL | 1.3139 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
