| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| 250mg |
|
||
| Other Sizes |
| Targets |
3-Azidopropylamine functions as a click chemistry reagent through its azide group. The azide group can participate in copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, a widely used click chemistry reaction for bioconjugation. The compound reacts with the starch sugar of potato starch for complexation and transfection of plasmid DNA. This reaction involves oxidation of potato starch amylose followed by reaction with 3-azidopropylamine in the presence of N,N'-carbonyldiimidazole. The resulting azide-functionalized starch can be used for plasmid DNA complexation and transfection. The compound's amine group provides additional functionality for conjugation reactions.
|
|---|---|
| ln Vitro |
In order to complex and transfect plasmid DNA, 3-Azidopropylamine and N, N'-carbonyldiimidazole are used to azidize the amylose from potato starch[1].
In vitro, 3-azidopropylamine is used as a reagent for click chemistry applications. It reacts with the starch sugar of potato starch for complexation and transfection of plasmid DNA. The compound's azide group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with alkyne-functionalized molecules. This allows for the creation of bioconjugates and functionalized materials. The compound is used in the complexation and transfection of plasmid DNA. Its amine group provides additional functionality for further derivatization. The compound's click chemistry properties make it valuable for various bioconjugation and materials science applications. Detailed in vitro protocols are described in the chemical and biochemical literature. |
| ln Vivo |
In vivo applications of 3-azidopropylamine are limited, as the compound is primarily used as a chemical reagent for in vitro click chemistry and bioconjugation. Its use in plasmid DNA complexation and transfection suggests potential for gene delivery applications. However, the compound's azide group and reactivity may limit its use in vivo due to potential toxicity or off-target reactions. The compound is intended for research use only and not for human therapeutic applications. Researchers should consult the primary literature for any available in vivo data. The compound's click chemistry properties are primarily exploited in vitro for bioconjugation, materials science, and chemical biology research.
|
| Enzyme Assay |
For in vitro biochemical assays, 3-azidopropylamine is evaluated for its click chemistry reactivity and bioconjugation applications. Azide-alkyne cycloaddition reactions are performed with alkyne-functionalized molecules, and reaction products are analyzed by mass spectrometry, NMR, or chromatography. DNA complexation and transfection efficiency are assessed using gel electrophoresis and reporter gene assays. The compound's purity and reactivity are characterized using standard chemical analysis methods. Its azide content is determined by titration or spectroscopic methods. These assays help characterize the compound's reactivity and suitability for click chemistry applications.
|
| Cell Assay |
In vitro cellular assays for 3-azidopropylamine are limited, as the compound is primarily used as a chemical reagent. However, its use in plasmid DNA complexation and transfection can be evaluated in cell culture. Cells are transfected with plasmid DNA complexed with azide-functionalized starch prepared using 3-azidopropylamine. Transfection efficiency is assessed by measuring reporter gene expression using fluorescence or luminescence assays. Cell viability is assessed using MTT or LDH release assays to evaluate cytotoxicity. The compound's effects on cell function are studied in the context of gene delivery applications. These cellular assays help validate the compound's utility for DNA delivery.
|
| Animal Protocol |
In vivo animal experiments with 3-azidopropylamine are not extensively documented, as the compound is primarily used as a chemical reagent. If used in animal studies, typical applications would involve gene delivery using azide-functionalized DNA complexes. The compound would be administered as part of a DNA delivery formulation via injection. Efficacy would be assessed by measuring transgene expression in target tissues. Toxicity would be monitored through body weight, clinical signs, and clinical chemistry. However, the compound's azide group and reactivity may limit its in vivo use. Researchers should consult the primary literature for any available in vivo data.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of 3-azidopropylamine are not extensively documented. As a small molecule (molecular weight ~100 g/mol) with an azide group, it is expected to be rapidly absorbed and distributed if administered systemically. However, its reactivity and potential toxicity may limit its use in pharmacokinetic studies. The compound is primarily used as a chemical reagent rather than a therapeutic agent, and its pharmacokinetic properties are not typically characterized. Researchers should consult the primary literature for any available data. The compound is intended for research use only.
|
| Toxicity/Toxicokinetics |
The toxicological profile of 3-azidopropylamine is not extensively characterized. As an azide-containing compound, it may have potential for toxicity due to the reactivity of the azide group. Azides can be toxic and may cause adverse effects if ingested, inhaled, or absorbed through the skin. The compound should be handled with appropriate safety precautions. Comprehensive toxicity studies including acute, subchronic, and chronic toxicity assessments are limited. The compound is intended for research use only and not for human therapeutic applications. Researchers should follow standard laboratory safety practices when handling 3-azidopropylamine, including the use of appropriate personal protective equipment and working in a fume hood.
|
| References | |
| Additional Infomation |
3-Azidopropylamine is a valuable research tool for click chemistry, bioconjugation, and DNA delivery applications. Its azide group enables copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, a versatile method for creating bioconjugates and functionalized materials. The compound is used for complexation and transfection of plasmid DNA, making it relevant for gene delivery and nucleic acid research. Its reaction with potato starch provides a method for creating biocompatible materials for DNA delivery. The compound's amine group provides additional functionality for further derivatization. 3-Azidopropylamine is also used in materials science for creating functionalized surfaces and polymers.
|
| Molecular Formula |
C3H8N4
|
|---|---|
| Molecular Weight |
100.12
|
| Exact Mass |
100.075
|
| CAS # |
88192-19-2
|
| PubChem CID |
150110
|
| Appearance |
Colorless to light yellow liquid
|
| Density |
1.020 g/cm3
|
| Boiling Point |
50 °C/15 mmHg
|
| Flash Point |
60 °C
|
| Index of Refraction |
1.46
|
| LogP |
0.798
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
7
|
| Complexity |
73
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[N-]=[N+]=NCCCN
|
| InChi Key |
OYBOVXXFJYJYPC-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C3H8N4/c4-2-1-3-6-7-5/h1-4H2
|
| Chemical Name |
3-azidopropan-1-amine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 100 mg/mL (998.80 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.9880 mL | 49.9401 mL | 99.8801 mL | |
| 5 mM | 1.9976 mL | 9.9880 mL | 19.9760 mL | |
| 10 mM | 0.9988 mL | 4.9940 mL | 9.9880 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
