| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
(2R)-Glycerol-O-β-D-galactopyranoside targets enzymes involved in carbohydrate metabolism, particularly glycosidases such as β-galactosidase, which hydrolyze the glycosidic bond to release galactose and glycerol. It may also be used as a substrate or inhibitor for studying glycosidase activity. The compound's specific biological targets and mechanism of action depend on the experimental context.
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| ln Vitro |
In vitro, (2R)-Glycerol-O-β-D-galactopyranoside is used as a substrate for β-galactosidase and other glycosidases in enzyme activity assays. The compound is hydrolyzed by the enzyme to release galactose, which can be quantified using colorimetric or fluorometric methods. It may also be used in studies of glycoside transport and metabolism in bacteria and other microorganisms. The compound's in vitro activity is primarily related to its use as a biochemical tool rather than as a pharmacologically active compound.
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| ln Vivo |
In vivo, (2R)-Glycerol-O-β-D-galactopyranoside may be used in studies of carbohydrate metabolism and glycoside absorption. However, specific in vivo studies on this compound are limited, as it is primarily used as a research reagent rather than a therapeutic agent. Its metabolism and disposition would be expected to follow pathways similar to other galactose-containing glycosides.
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| Enzyme Assay |
The in vitro assays for (2R)-Glycerol-O-β-D-galactopyranoside typically involve measuring β-galactosidase activity. The compound is incubated with the enzyme in assay buffer at 37°C for a set period, and the released galactose is quantified using a galactose oxidase/peroxidase assay or by HPLC. Alternatively, the compound may be used as a substrate in colorimetric assays where the release of galactose is coupled to a chromogenic reaction. The assay is performed in 96-well plates with varying concentrations of the substrate to determine kinetic parameters (Km, Vmax). Positive controls (e.g., other glycosidase substrates) and negative controls (without enzyme) are included in each assay run.
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| Cell Assay |
For in vitro cellular assays, (2R)-Glycerol-O-β-D-galactopyranoside may be used in cell culture studies to investigate glycoside transport or metabolism. Cells are treated with the compound, and the accumulation of galactose or glycerol in the media or cells is measured by HPLC or enzymatic assays. The compound may also be used to study the effects of glycosides on cell function, though specific protocols vary depending on the study objectives.
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| Animal Protocol |
In vivo studies for (2R)-Glycerol-O-β-D-galactopyranoside are not well-documented. If performed, the compound would be administered to animals via oral or intravenous routes, and its metabolism and excretion would be studied. However, specific protocols are not available in publicly accessible literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for (2R)-Glycerol-O-β-D-galactopyranoside are not available in publicly accessible literature. Its absorption, distribution, metabolism, and excretion have not been characterized.
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| Toxicity/Toxicokinetics |
Toxicology data for (2R)-Glycerol-O-β-D-galactopyranoside are not available. As a naturally occurring glycoside, it is expected to have low toxicity, but comprehensive toxicology studies have not been performed.
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| Additional Infomation |
3-O-β-D-galactopyranosyl-sn-glycerol is a D-galactosylglycerol. It is a human metabolite. It has been reported to be present in chili peppers, Homo sapiens, and other organisms with relevant data.
(2R)-Glycerol-O-β-D-galactopyranoside is a research compound used as a substrate for glycosidase studies and as a tool for studying carbohydrate metabolism. It is not approved for human use and is intended for research purposes only. The compound is available as a research-grade reagent for laboratory use. |
| Molecular Formula |
C9H18O8
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|---|---|
| Molecular Weight |
254.23442
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| Exact Mass |
254.1
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| CAS # |
16232-91-0
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| PubChem CID |
16048618
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| Appearance |
Colorless to light yellow oil
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| Index of Refraction |
1.592
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| LogP |
-3.4
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
17
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| Complexity |
225
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| Defined Atom Stereocenter Count |
6
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| SMILES |
C([C@H](CO[C@H]1[C@@H]([C@H]([C@H]([C@@H](CO)O1)O)O)O)O)O
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| InChi Key |
NHJUPBDCSOGIKX-NTXXKDEISA-N
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| InChi Code |
InChI=1S/C9H18O8/c10-1-4(12)3-16-9-8(15)7(14)6(13)5(2-11)17-9/h4-15H,1-3H2/t4-,5-,6+,7+,8-,9-/m1/s1
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| Chemical Name |
(2R,3R,4S,5R,6R)-2-[(2R)-2,3-dihydroxypropoxy]-6-(hydroxymethyl)oxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9334 mL | 19.6672 mL | 39.3345 mL | |
| 5 mM | 0.7867 mL | 3.9334 mL | 7.8669 mL | |
| 10 mM | 0.3933 mL | 1.9667 mL | 3.9334 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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