| Size | Price | Stock | Qty |
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| Targets |
The compound is a sterol derivative used as a biochemical standard and reference material for analytical testing. As a sterol, it is involved in the biosynthesis of steroids, particularly in the ergosterol biosynthetic pathway in fungi and the cholesterol biosynthetic pathway in animals. It does not target a specific protein for pharmacological activity; rather, it serves as an intermediate or byproduct in sterol synthesis, functioning as a reference compound for understanding sterol metabolism and membrane dynamics.
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| ln Vitro |
The compound is not a drug and does not exert pharmacological activity. It is used as a standard for analytical testing and for studies of sterol metabolism and membrane behavior. It is a sterol derivative of episterol and is used in research on the biosynthesis of steroids, particularly as a reference standard for chromatographic analysis (e.g., HPLC, GC-MS) and lipidomics studies.
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| ln Vivo |
Not applicable. 24(28)-Dehydroergosterol is a biochemical reference compound and is not administered as a therapeutic agent in animal models. It is used as a standard for analytical testing in biochemical research, and its “in vivo activity” is not relevant, as it is not studied for therapeutic efficacy. It is used in studies of sterol metabolism, where its levels in animal or fungal tissues are measured as a marker of sterol biosynthesis activity.
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| Enzyme Assay |
Not applicable. This compound is a sterol standard and is not used in enzyme/receptor binding assays. Instead, it is used as a reference standard in analytical methods. A typical application is in GC-MS analysis: the compound is dissolved in an organic solvent (e.g., methanol or chloroform), derivatized (e.g., silylation with BSTFA), and injected onto a gas chromatograph equipped with a mass spectrometer. The retention time and mass spectrum of the sample are compared to that of the reference standard for identification and quantification.
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| Cell Assay |
As a sterol standard, the compound is not used for cell-based viability assays. It can be used in lipidomics studies to analyze the sterol composition of cells. A typical protocol involves extracting total lipids from cell pellets using a chloroform/methanol/water mixture, followed by saponification (if analyzing total sterols) and purification. The sterol extract is then analyzed by HPLC with UV detection or by GC-MS. The retention time of the sample peak is compared to that of the reference standard, and the concentration is calculated based on a calibration curve.
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| Animal Protocol |
In vivo animal experiments are not applicable for this compound as a therapeutic. However, in metabolic tracer studies, the compound can be used as a reference standard. A protocol for sterol metabolism studies involves administering stable isotope-labeled sterol precursors (e.g., 13C-acetate or 2H2O) to mice, collecting tissues (liver, plasma, brain) at various time points, extracting sterols, and analyzing by GC-MS. The compound serves as an external or internal standard for quantifying the biosynthetic products and intermediates of the cholesterol or ergosterol pathways.
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| ADME/Pharmacokinetics |
As a sterol reference compound, pharmacokinetic properties are not applicable. The compound is a naturally occurring sterol with physical properties characteristic of steroids: it is highly lipophilic, insoluble in water, and soluble in organic solvents such as chloroform, methanol, and DMSO. It is used as an analytical standard, not as an administered drug.
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| Toxicity/Toxicokinetics |
The compound is a naturally occurring sterol and is considered to have low toxicity at the concentrations used for analytical standards. When used as a reference material, standard laboratory safety practices for handling organic compounds should be followed. It is not intended for human or veterinary use, and no toxicological studies are required for its use as a research reagent.
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| References | |
| Additional Infomation |
Ergoster-5,7,22,24(28)-tetraen-3β-ol is a 3β-sterol with double bonds at positions 5, 7, and 22, and a methylene group at position 24. It is a metabolite of Saccharomyces cerevisiae. It is a 3β-sterol and a 3β-hydroxy-Δ(5)-sterol compound. It is derived from the hydride of 5α-ergosterane. Ergoster-5,7,22,24(28)-tetraen-3β-ol has been reported in cyanobacteria, Arabidopsis thaliana, and other organisms with relevant data. Ergoster-5,7,22,24(28)-tetraen-3β-ol is a metabolite found or produced in Saccharomyces cerevisiae.
24(28)-Dehydroergosterol is a research-grade reference standard and is not a drug candidate. It is an essential tool for lipidomics and sterol research, enabling the accurate quantification and identification of sterol intermediates in biosynthetic pathways. It is used in studies of fungal ergosterol biosynthesis for antifungal drug development, in studies of cholesterol metabolism for cardiovascular disease research, and in membrane biology for understanding sterol effects on membrane fluidity and permeability. As of the latest updates, it has not been approved for clinical use. |
| Molecular Formula |
C28H42O
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|---|---|
| Molecular Weight |
394.63248872757
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| Exact Mass |
394.324
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| CAS # |
29560-24-5
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| PubChem CID |
11090531
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| Appearance |
White to off-white solid powder
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| LogP |
7.25
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
29
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| Complexity |
752
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| Defined Atom Stereocenter Count |
7
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| SMILES |
C[C@H](/C=C/C(=C)C(C)C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3C2=CC=C4[C@@]3(CC[C@@H](C4)O)C)C
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| InChi Key |
SQFQJKZSFOZDJY-CVGLIYDESA-N
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| InChi Code |
InChI=1S/C28H42O/c1-18(2)19(3)7-8-20(4)24-11-12-25-23-10-9-21-17-22(29)13-15-27(21,5)26(23)14-16-28(24,25)6/h7-10,18,20,22,24-26,29H,3,11-17H2,1-2,4-6H3/b8-7+/t20-,22+,24-,25+,26+,27+,28-/m1/s1
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| Chemical Name |
(3S,9S,10R,13R,14R,17R)-10,13-dimethyl-17-[(E,2R)-6-methyl-5-methylidenehept-3-en-2-yl]-2,3,4,9,11,12,14,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5340 mL | 12.6701 mL | 25.3402 mL | |
| 5 mM | 0.5068 mL | 2.5340 mL | 5.0680 mL | |
| 10 mM | 0.2534 mL | 1.2670 mL | 2.5340 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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