| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| ln Vitro |
1. Effect on glutamate uptake in rat brain slices: Rat cerebral cortex slices were incubated in Krebs-Ringer buffer containing 2,3-Diaminopropionic acid (1 mM, 5 mM) for 30 minutes. The uptake of [³H]-labeled L-glutamate by the slices was measured using a liquid scintillation counter. Results showed that 2,3-Diaminopropionic acid did not inhibit glutamate uptake at the tested concentrations, whereas the positive control (β-ODAP, 1 mM) significantly reduced glutamate uptake by (35.2 ± 4.1)% [1]
2. Effect on brain slice viability: After incubating rat brain slices with 2,3-Diaminopropionic acid (1 mM, 5 mM) for 2 hours, the lactate dehydrogenase (LDH) release rate was detected. No significant increase in LDH release was observed, indicating that 2,3-Diaminopropionic acid had no obvious cytotoxic effect on brain slices under the experimental conditions [1] |
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| ln Vivo |
1. Acute toxicity in mice: Swiss albino mice (20-25 g) were administered 2,3-Diaminopropionic acid via intraperitoneal injection at doses of 200 mg/kg and 400 mg/kg. The mice were observed continuously for 24 hours. No neurotoxic symptoms (such as convulsions, ataxia, paralysis) or death were found in the 2,3-Diaminopropionic acid-treated groups, while mice injected with β-ODAP (100 mg/kg) showed severe convulsions and 80% mortality within 12 hours [1]
2. Effect on brain glutamate content: Mice were sacrificed 24 hours after intraperitoneal injection of 2,3-Diaminopropionic acid (400 mg/kg). The cerebral cortex was homogenized, and glutamate content was measured by high-performance liquid chromatography (HPLC). No significant difference in brain glutamate levels was found between the 2,3-Diaminopropionic acid group and the normal saline control group, whereas β-ODAP (100 mg/kg) significantly increased brain glutamate content by (42.5 ± 5.3)% [1] |
| Animal Protocol |
1. Mouse acute toxicity experiment: Swiss albino mice (n=6 per group, male and female in half, weight 20-25 g) were acclimated for 3 days before the experiment. 2,3-Diaminopropionic acid was dissolved in normal saline to prepare solutions with concentrations of 20 mg/mL and 40 mg/mL. The drug was administered via intraperitoneal injection at doses of 200 mg/kg (10 mL/kg body weight) and 400 mg/kg (10 mL/kg body weight); the control group received an equal volume of normal saline. After administration, the mice were placed in individual cages, and their behavioral changes (such as movement, posture, response to stimuli) and mortality were recorded every 2 hours for 24 hours [1]
2. Mouse brain glutamate detection experiment: After 24 hours of 2,3-Diaminopropionic acid (400 mg/kg) administration, mice were sacrificed by cervical dislocation. The cerebral cortex was quickly dissected on an ice-cold plate, weighed, and homogenized in 5 volumes of ice-cold perchloric acid (0.4 M) to precipitate proteins. The homogenate was centrifuged at 12,000 × g for 15 minutes at 4°C, and the supernatant was neutralized with potassium hydroxide. The neutralized supernatant was filtered through a 0.22 μm membrane, and glutamate content was determined by HPLC with a UV detector [1] |
| Toxicity/Toxicokinetics |
1. Acute toxicity: No neurotoxic symptoms (convulsions, ataxia, paralysis) or death were observed in mice after intraperitoneal injection of up to 400 mg/kg of 2,3-diaminopropionic acid within 24 hours. [1]
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| References | |
| Additional Infomation |
3-Amino-L-alanine is a 3-aminoalanine with an S-configuration. It is a derivative of L-alanine, a non-protein L-α-amino acid, and also a 3-aminoalanine. It is the conjugate base of 3-amino-L-alanine (1+) and 3-amino-L-alanine. It is the conjugate acid of 3-amino-L-alanine. It is the enantiomer of 3-amino-D-alanine. It is the zwitterion tautomer of 3-amino-L-alanine. (S)-2,3-diaminopropionate has been reported in Coniophora puteana, and relevant data are available. 2,3-Diaminopropionic acid is a non-protein amino acid isolated from the seeds of Lathyrus sativus, and it is a structural analog of the neurotoxin β-N-oxalyl-L-αβ-diaminopropionic acid (β-ODAP). This study used 2,3-diaminopropionic acid as a control to confirm that the neurotoxicity of wild pea is caused by β-ODAP specifically, rather than by its structural analogue 2,3-diaminopropionic acid [1].
|
| Molecular Formula |
C3H8N2O2
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|---|---|
| Molecular Weight |
104.10782
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| Exact Mass |
104.058
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| CAS # |
4033-39-0
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| Related CAS # |
2,3-Diaminopropionic acid hydrochloride;1482-97-9
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| PubChem CID |
97328
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
325.6±32.0 °C at 760 mmHg
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| Flash Point |
150.7±25.1 °C
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| Vapour Pressure |
0.0±1.5 mmHg at 25°C
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| Index of Refraction |
1.522
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| LogP |
-1.74
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
7
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| Complexity |
73.3
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| Defined Atom Stereocenter Count |
1
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| SMILES |
N[C@@H](CN)C(O)=O
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| InChi Key |
PECYZEOJVXMISF-REOHCLBHSA-N
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| InChi Code |
InChI=1S/C3H8N2O2/c4-1-2(5)3(6)7/h2H,1,4-5H2,(H,6,7)/t2-/m0/s1
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| Chemical Name |
(2S)-2,3-diaminopropanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.6052 mL | 48.0261 mL | 96.0523 mL | |
| 5 mM | 1.9210 mL | 9.6052 mL | 19.2105 mL | |
| 10 mM | 0.9605 mL | 4.8026 mL | 9.6052 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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