| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The compound is a substrate for the enzyme 2-deoxyribose-5-phosphate aldolase (DERA), a class I aldolase that catalyzes the reversible aldol condensation of acetaldehyde with glyceraldehyde-3-phosphate to form 2-deoxyribose-5-phosphate. It is also a substrate for deoxyribokinase and deoxyribonucleoside kinases and plays a role in the cellular metabolic network regulating nucleotide pools for DNA replication and repair.
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| ln Vitro |
As a natural metabolite, 2-deoxyribose 5-phosphate disodium does not exert pharmacological activity. Instead, it serves as an essential substrate for biochemical and enzymatic reactions. Its primary "activity" is its ability to be converted by DERA into other metabolites, forming the basis of the deoxyribonucleotide salvage pathway. It is widely used as a starting material for the chemoenzymatic preparation of natural and modified purine and pyrimidine deoxynucleosides.
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| ln Vivo |
This compound is not a drug and is not used for in vivo activity studies in the context of treating a disease. It is a research tool for studying enzymatic mechanisms and for the in vitro synthesis of deoxynucleosides. Its "in vivo activity" is limited to its role as a natural metabolite, but it is not administered for any therapeutic effect. Exogenously administered compound would be rapidly metabolized via normal catabolic pathways.
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| Enzyme Assay |
To measure DERA activity, an enzyme assay is performed in a buffer containing 2-deoxyribose 5-phosphate disodium as the substrate. The purified DERA enzyme, along with varying concentrations of the compound, is incubated in a suitable buffer (e.g., 50 mM Tris-HCl, pH 7.5). The reaction is initiated by adding the substrate and is carried out at 37degC. The conversion of the substrate to products (acetaldehyde and glyceraldehyde-3-phosphate) is measured indirectly by a coupled enzyme assay using triose phosphate isomerase and alpha-glycerophosphate dehydrogenase, monitoring the oxidation of NADH at 340 nm.
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| Cell Assay |
As a cellular metabolite, the compound is not used for direct cell stimulation. Instead, it is used as a reagent in cell-free systems or as a supplement in culture media for certain microorganisms. For mammalian cells, the compound can be used in metabolic labeling studies where cells are cultured with stable isotope-labeled 2-deoxyribose 5-phosphate to trace the metabolic flux of the deoxyribonucleotide salvage pathway via LC-MS analysis.
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| Animal Protocol |
In vivo animal experiments are not applicable for this compound, as it is a research reagent for biochemical and enzymological studies. If used in an animal model, it would be part of a pharmacokinetic tracing study using a stable isotope-labeled version to study the absorption, distribution, and metabolism of deoxyribose phosphates. A protocol would involve intravenous administration of the labeled compound to mice, followed by serial blood collection and LC-MS analysis.
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| ADME/Pharmacokinetics |
2-Deoxyribose 5-phosphate disodium is a small, highly polar, water-soluble molecule. Its pharmacokinetics are similar to that of a naturally occurring metabolite; it would be rapidly cleared from the systemic circulation via renal filtration and cellular uptake. Specific quantitative PK parameters are not studied or required for this compound, as it is not a therapeutic candidate. Its primary properties of interest are solubility and enzymatic stability.
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| Toxicity/Toxicokinetics |
2-Deoxyribose 5-phosphate disodium is an endogenous metabolite and is considered non-toxic at physiological concentrations. In research settings, standard laboratory safety practices are sufficient. High systemic concentrations are not expected to be toxic but could potentially disrupt normal nucleotide pool balance. Its primary use is as a biochemical tool, not as a therapeutic, and formal toxicological studies are not routinely performed for its use as a reagent.
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| References | |
| Additional Infomation |
2-Deoxyribose 5-phosphate disodium is a fundamental research tool in biochemistry and enzymology. It is the natural substrate for the class I aldolase DERA, an enzyme that has garnered industrial interest for its use in the "de novo" synthesis of novel sugars and complex molecules via carbon-carbon bond formation. Therefore, this compound is not a drug but a key starting material and substrate for applied biocatalysis and synthetic biology.
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| Molecular Formula |
C5H9NA2O7P
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|---|---|
| Molecular Weight |
258.07
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| Exact Mass |
234.998
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| CAS # |
102916-66-5
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| PubChem CID |
87357745
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
15
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| Complexity |
188
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C(C=O)[C@@H]([C@@H](COP(=O)([O-])[O-])O)O.[Na+].[Na+]
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~387.49 mM)
H2O : ~83.33 mg/mL (~322.90 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8749 mL | 19.3746 mL | 38.7492 mL | |
| 5 mM | 0.7750 mL | 3.8749 mL | 7.7498 mL | |
| 10 mM | 0.3875 mL | 1.9375 mL | 3.8749 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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