| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
| Targets |
2'-Deoxyadenosine inhibits the activity of S-adenosyl-L-homocysteine hydrolase (SAHH). It inhibits glucose-stimulated increases in islet cyclic AMP (cAMP) accumulation. It activates caspase-3 and promotes apoptosis.
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|---|---|
| ln Vitro |
In vitro, 2'-deoxyadenosine (0.01-0.5 mM; 24-72 h) inhibits the growth of LoVo human colon carcinoma cells in a concentration- and time-dependent manner and promotes apoptosis, as indicated by characteristic apoptotic morphology. It also reduces islet cAMP accumulation.
|
| ln Vivo |
In vivo, 2'-deoxyadenosine is used in paradoxical stimulation studies of human sperm motility. It plays an essential role as a sperm motility stimulant used in sperm micro-injection on mouse and human embryo development. Its anticancer effects have been observed in colon cancer models.
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| Enzyme Assay |
Cell-free assays for 2'-deoxyadenosine involve measuring its inhibition of SAHH activity. The enzyme is incubated with the compound and its substrate, and the formation of products is quantified using HPLC or spectrophotometric methods to determine the inhibitory potency.
|
| Cell Assay |
In vitro cytotoxicity assays are performed using cancer cell lines such as LoVo human colon carcinoma cells. Cells are treated with varying concentrations of 2'-deoxyadenosine for 24-72 hours, and cell viability is measured using MTT or similar assays. Apoptosis is confirmed by morphological examination and caspase-3 activity measurement.
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| Animal Protocol |
In vivo studies have been conducted in mouse and human embryo development models. 2'-Deoxyadenosine is used as a sperm motility stimulant in sperm micro-injection procedures. The compound's effects on development and fertility are evaluated by monitoring embryo development and pregnancy outcomes.
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| ADME/Pharmacokinetics |
2'-Deoxyadenosine (C10H13N5O3) has a molecular weight of 251.25 g/mol. It is a nucleoside that is a component of DNA. Its physical properties and stability are typical of nucleosides; it is generally stored at -20°C and is soluble in aqueous solutions.
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| Toxicity/Toxicokinetics |
The toxicity of 2'-deoxyadenosine is related to its ability to induce apoptosis and inhibit cell proliferation. Its effects on rapidly dividing cells, such as cancer cells and immune cells, have been studied. Detailed toxicological data, including organ-specific toxicity and systemic effects, are limited to research applications.
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| Additional Infomation |
2'-Deoxyadenosine is a purine 2'-deoxynucleoside with adenine as its nucleobase. It is a metabolite found in humans, as well as in Saccharomyces cerevisiae, Escherichia coli, and mice. It is a purine 2'-deoxy-D-ribonucleoside. Deoxyadenosine is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). It has been reported in Fritillaria przewalskii, humans, and other organisms with relevant data. 2'-Deoxyadenosine is a purine nucleoside component of DNA, formed by adenosine linked to the C1 carbon atom of deoxyribose via its N9 nitrogen atom. Deoxyadenosine is a derivative of the nucleoside adenosine. It is formed by adenine linked to the deoxyribose portion via an N9-glycosidic bond. The difference between deoxyribose and ribose is that deoxyribose lacks an oxygen atom at the 3' position of its ribose ring. Deoxyadenosine is an important component of DNA. 2'-Deoxyadenosine is a metabolite found or produced in Saccharomyces cerevisiae.
2'-Deoxyadenosine is used in paradoxical stimulation studies of human sperm motility. It plays an essential role as a sperm motility stimulant used in sperm micro-injection on mouse and human embryo development. It is a nucleoside adenosine derivative that pairs with deoxythymidine (T) in double-stranded DNA. |
| Molecular Formula |
C10H13N5O3
|
|---|---|
| Molecular Weight |
251.25
|
| Exact Mass |
251.101
|
| CAS # |
958-09-8
|
| PubChem CID |
13730
|
| Appearance |
White to off-white solid powder
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| Density |
1.9±0.1 g/cm3
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| Boiling Point |
627.2±65.0 °C at 760 mmHg
|
| Melting Point |
187-189ºC
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| Flash Point |
333.1±34.3 °C
|
| Vapour Pressure |
0.0±1.9 mmHg at 25°C
|
| Index of Refraction |
1.863
|
| LogP |
-0.55
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
18
|
| Complexity |
307
|
| Defined Atom Stereocenter Count |
3
|
| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=NC3=C(N=CN=C32)N)CO)O
|
| InChi Key |
OLXZPDWKRNYJJZ-RRKCRQDMSA-N
|
| InChi Code |
InChI=1S/C10H13N5O3/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(17)6(2-16)18-7/h3-7,16-17H,1-2H2,(H2,11,12,13)/t5-,6+,7+/m0/s1
|
| Chemical Name |
(2R,3S,5R)-5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol
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| Synonyms |
NSC 141848 NSC1418482'-Deoxyadenosine NSC-141848
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9801 mL | 19.9005 mL | 39.8010 mL | |
| 5 mM | 0.7960 mL | 3.9801 mL | 7.9602 mL | |
| 10 mM | 0.3980 mL | 1.9900 mL | 3.9801 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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