| Size | Price | Stock | Qty |
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| 5mg |
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| Other Sizes |
| Targets |
As a deuterium-labeled internal standard, 2,6-Dimethylaniline-d6 HCl does not have a specific pharmacological target. 2,6-Dimethylaniline is an aromatic amine used as an intermediate in the synthesis of various chemicals, including pharmaceuticals, dyes, and pesticides. The deuterated form is used as an analytical standard for the quantification of 2,6-dimethylaniline in biological and environmental samples.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
2,6-Dimethylaniline-d6 HCl is used in vitro as an internal standard for the quantification of 2,6-dimethylaniline. The compound does not exhibit intrinsic pharmacological activity but serves as a quantitative tool for analytical method development and validation. Stable heavy isotopes have been incorporated into drug molecules largely as tracers for quantitation during the drug development process. |
| ln Vivo |
In vivo, 2,6-Dimethylaniline-d6 HCl is used as an internal standard to trace the absorption, distribution, metabolism, and excretion of 2,6-dimethylaniline. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry.
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| Enzyme Assay |
As an internal standard, 2,6-Dimethylaniline-d6 HCl is used in analytical assays such as GC-MS or LC-MS. Typical protocols involve spiking the labeled compound into samples prior to extraction and analysis. The labeled compound co-elutes with the unlabeled 2,6-dimethylaniline but is detected at a different mass-to-charge ratio, allowing for precise quantification and correction for matrix effects.
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| Cell Assay |
In vitro cell culture experiments using 2,6-Dimethylaniline-d6 HCl involve supplementing growth media with the labeled compound at defined concentrations for analytical method development. The compound can be used to study the metabolism and transport of 2,6-dimethylaniline in various cell types when used as a tracer.
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| Animal Protocol |
In vivo animal studies using 2,6-Dimethylaniline-d6 HCl involve administering the compound to rodents via oral gavage or intravenous injection. Blood, urine, and tissue samples are collected at various time points, and the concentration of the labeled compound is measured by mass spectrometry.
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| ADME/Pharmacokinetics |
2,6-Dimethylaniline-d6 HCl exhibits pharmacokinetic properties similar to those of unlabeled 2,6-dimethylaniline hydrochloride. As an aromatic amine, it is absorbed from the gastrointestinal tract and distributed throughout the body. It is metabolized primarily in the liver and excreted in urine. The deuterium label provides a distinct mass shift for analytical detection.
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| Toxicity/Toxicokinetics |
2,6-Dimethylaniline-d6 HCl is considered safe for research use at typical concentrations. The compound is non-hazardous for transport. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References | |
| Additional Infomation |
2,6-Dimethylaniline-d6 hydrochloride has a molecular formula of C8H6D6ClN and a molecular weight of 163.68. Purity is typically ≥98%. Isotopic enrichment is typically 98 atom% D. The compound appears as a solid at room temperature and is primarily used as an internal standard for mass spectrometry-based quantification of 2,6-xylidine.
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| Molecular Formula |
C8H12CLN
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|---|---|
| Molecular Weight |
163.677551269531
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| CAS # |
2748480-14-8
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| Appearance |
Typically exists as solid at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.1095 mL | 30.5474 mL | 61.0948 mL | |
| 5 mM | 1.2219 mL | 6.1095 mL | 12.2190 mL | |
| 10 mM | 0.6109 mL | 3.0547 mL | 6.1095 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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