| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
2',4'-Dihydroxy-6'-methoxyacetophenone targets microbial pathogens, exhibiting antimicrobial activity. Its phenolic structure enables radical scavenging and reduction of oxidative stress. As an analog of artemisinin, it may share some antimalarial properties. The compound may interact with various cellular targets including enzymes involved in oxidative stress, microbial metabolism, and inflammatory pathways. Specific molecular targets are not extensively characterized.
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| ln Vitro |
2',4'-Dihydroxy-6'-methoxyacetophenone exhibits antimicrobial activity. It shows antioxidant activity through radical scavenging mechanisms. As an analog of artemisinin, it may have antimalarial potential. Specific IC₅₀ values for various activities are not extensively documented in the literature. The compound is used in the synthesis of pyran-sickle derivatives and as a research tool for studying phenolic compounds.
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| ln Vivo |
In vivo activity data for 2',4'-Dihydroxy-6'-methoxyacetophenone are limited in the literature. Based on its in vitro antimicrobial and antioxidant activities, it is anticipated to have potential in vivo efficacy in models of infection and oxidative stress. However, specific animal studies detailing its therapeutic effects and pharmacokinetics are not extensively documented.
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| Enzyme Assay |
The non-cellular enzyme/receptor binding assays for 2',4'-Dihydroxy-6'-methoxyacetophenone would typically involve antioxidant activity evaluation using chemical assays such as DPPH, ABTS, and FRAP. The compound's ability to scavenge free radicals is measured spectrophotometrically. Antimicrobial activity can be assessed using broth microdilution assays against bacterial and fungal strains. The compound is incubated with the microorganism, and minimum inhibitory concentrations (MICs) are determined.
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| Cell Assay |
In vitro cellular assays for 2',4'-Dihydroxy-6'-methoxyacetophenone typically use bacterial and fungal cultures to evaluate antimicrobial activity. MICs are determined by broth microdilution. For antioxidant activity, cell-based assays using macrophages or other cell lines can be employed. Cells are treated with the compound and exposed to oxidative stress. Intracellular ROS levels are measured using fluorescent probes such as DCFH-DA. Cell viability is assessed using MTT assays.
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| Animal Protocol |
In vivo animal studies for 2',4'-Dihydroxy-6'-methoxyacetophenone are not extensively documented. Based on its antimicrobial and antioxidant activities, typical study designs would involve mouse models of bacterial or fungal infection, or models of oxidative stress-related diseases. The compound would be administered orally or intraperitoneally. Efficacy endpoints would include microbial load, oxidative stress markers, and histopathological analysis.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2',4'-Dihydroxy-6'-methoxyacetophenone are not extensively characterized. As a small phenolic compound with molecular weight 182.17, it is expected to have reasonable oral absorption. Metabolic pathways likely involve phase II conjugation (glucuronidation and sulfation) of the phenolic hydroxyl groups, as well as phase I oxidation of the methoxy and acetyl groups. Specific PK parameters such as half-life, Cmax, and bioavailability are not well documented.
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| Toxicity/Toxicokinetics |
Toxicological data for 2',4'-Dihydroxy-6'-methoxyacetophenone are limited. As a naturally occurring phenolic compound, it is generally considered to have low toxicity. However, comprehensive toxicology studies are not available. The compound may cause skin and eye irritation upon contact. Standard laboratory safety precautions should be observed when handling this compound.
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| References | |
| Additional Infomation |
2',4'-Dihydroxy-6'-methoxyacetophenone is an aromatic ketone. It has been reported to exist in Artemisia, Chrysanthemum, and other organisms with relevant data.
2',4'-Dihydroxy-6'-methoxyacetophenone is a research-grade natural product intended for laboratory use only. It is not approved for clinical use as a therapeutic agent. Its primary applications include studying the antimicrobial and antioxidant activities of phenolic acetophenones, investigating the chemistry and bioactivity of Artemisia annua constituents, and serving as a precursor for the synthesis of pyran-sickle derivatives. It is also used as a reference standard in natural product research. |
| Molecular Formula |
C9H10O4
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|---|---|
| Molecular Weight |
182.1733
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| Exact Mass |
182.057
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| CAS # |
3602-54-8
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| PubChem CID |
10965145
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
333.5±22.0 °C at 760 mmHg
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| Melting Point |
197 - 199 °C
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| Flash Point |
135.5±15.8 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.573
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| LogP |
2.19
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
13
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| Complexity |
192
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=O)C1=C(C=C(C=C1OC)O)O
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| InChi Key |
IETZAWFZIAOWQX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H10O4/c1-5(10)9-7(12)3-6(11)4-8(9)13-2/h3-4,11-12H,1-2H3
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| Chemical Name |
1-(2,4-dihydroxy-6-methoxyphenyl)ethanone
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4894 mL | 27.4469 mL | 54.8938 mL | |
| 5 mM | 1.0979 mL | 5.4894 mL | 10.9788 mL | |
| 10 mM | 0.5489 mL | 2.7447 mL | 5.4894 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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