Absorption, Distribution and Excretion
This study evaluated life-stage-dependent toxicity and dose-dependent toxicokinetics (TK) in Sprague Dawley rats following dietary intake of 2,4-dichlorophenoxyacetic acid (2,4-D). Renal clearance of 2,4-D is affected by dose-dependent saturation of renal organic anion transporters; therefore, this study aimed to determine the inflection point of the nonlinear dietary TK to guide dose selection in toxicity studies. Male and female rats were fed a 2,4-D-fortified diet at doses up to 1600 ppm for 4 weeks prior to mating, <2 weeks during mating, and from parental (P1) male rats to test day (TD) 71, and from P1 female rats during gestation/lactation to TD 96. F1 offspring were exposed to 2,4-D via milk and continued to be fed this diet until day 35 postnatal (PND 35). Nonlinear pharmacokinetic (TK) was observed in male P1 rats at concentrations ≥1200 ppm (63 mg/kg/day) and in female P1 rats at concentrations 200–400 ppm (14–27 mg/kg/day). 2,4-D levels in maternal milk and pup plasma were higher on day 14 of lactation (LD14) than on day 4 of lactation (LD4). 2,4-D levels were higher in mothers during late gestation/lactation and post-weaning pups (PND 21–35) compared to adult P1 rats, consistent with increased intake per kilogram of body weight. Dosage selection using the conventional maximum tolerated dose (MTD) criterion based on body weight variation would have resulted in a maximum dose approximately twice that determined after incorporating key TK data. These data suggest that if a nonlinear dose-kinetic kinase (TK) exists at dose levels far above the actual human exposure dose, confirmation of a nonlinear TK is a key dose-selection consideration for improving the human relevance of toxicity studies compared to studies employing conventional MTD dose-selection strategies. 2,4-D is distributed throughout the body, but there is no evidence of its accumulation. In mammals, the conversion of 2,4-D appears to be low, primarily involving the formation of 2,4-D conjugates with sugars or amino acids. Following a single dose, the drug is excreted over several days, primarily in the urine, with smaller amounts excreted in bile and feces. Pretreatment of rats with 2,4-D (250 mg/kg, subcutaneous injection) resulted in significant occupation of binding sites on plasma proteins, leading to altered distribution of 2,4-D upon intravenous injection 3.5 to 4.5 hours later compared to the control group. Concentrations in plasma and kidneys were decreased, while concentrations in the liver, brain, cerebrospinal fluid, testes, lungs, heart, and muscles were increased.
...2,4-D is primarily excreted in urine by the human body. Plasma clearance time depends on the dose, individual characteristics, and the presence of compounds that may competitively inhibit 2,4-D excretion. Following a single oral dose of 2,4-D, its biological half-life in plasma is approximately one day, with the exact time depending on various factors. However, forced alkaline diuresis can shorten this time to 3.7 hours.
For more complete data (36 items) on the absorption, distribution, and excretion of 2,4-D, please visit the HSDB records page.

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Metabolism/Metabolites
In studies using Arthrobacter spp. enzyme preparations, 2,4-D was converted to 2,4-D-phenol and glyoxylic acid. The two glyoxylic acid molecules undergo condensation, with one carboxyl group losing CO2. A compound identical to alanine was observed chromatographically. Using ring-labeled 2,4-D, labeled succinic acid was generated.
2,4-D esters are hydrolyzed in animals. Phenoxy acid compounds are primarily excreted unchanged in rat urine after oral administration, with small amounts conjugated with the amino acids glycine, taurine, and glucuronic acid. Soybean root callus cultures can metabolize 2,4-D. Identified metabolites include 2,4-D-glutamic acid and 2,4-D-aspartic acid conjugates; other unidentified 2,4-D amino acid conjugates; 2,5-dichloro-4-hydroxyphenoxyacetic acid (4-OH-2,5-D); and 5-OH-2,4-D… No qualitative differences were observed in the metabolism of 2,4-D in soybean callus, soybean plants, and maize plants. Hydroxyl compounds exist mainly as glycosides and have been identified as 5-OH-2,4-D, 4-OH-2,3-D, and 4-OH-2,5-D. Amino acid conjugates have been identified as 2,4-D conjugates of aspartic acid, glutamic acid, alanine, valine, phenylalanine, tryptophan, and leucine. Some data indicate the presence of amino acid conjugates of cyclically hydroxylated 2,4-D. /SRP: Unspecified salt or ester of 2,4-D/
A single dose of 5 mg/kg was administered to male volunteers. Excretion was primarily in the form of 2,4-D (82.3%), with a small amount in the form of 2,4-D conjugates (12.8%). /SRP: Unspecified salt or ester of 2,4-D/
For more complete data on the metabolism of 2,4-D (7 metabolites in total), please visit the HSDB record page.
2,4-D is minimally metabolized in the human body and is almost entirely excreted unchanged. In particular, 2,4-D is rapidly excreted from the body, primarily through urine. While some 2,4-D is excreted as conjugates, the majority of the compound appears to be excreted unchanged. 2,4-D is metabolized to 2,4-dichlorophenol (2,4-DCP) by cytochrome P450 3A4 (CYP 3A4), the main monooxygenase in human liver.

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Biological half-life
Systemic: 220 hours (can be shortened to 4-7 hours by urinary alkalinity); Mean plasma half-life: 12 hours; [TDR, p. 510]
…The urinary excretion half-life in rats is 3 hours, in calves and hens it is 8 hours, and in pigs it is approximately 12 hours.
…After oral or intravenous injection of 2,4-D in rats, it is mainly excreted in urine, with a half-life of approximately 2 hours.
Six male volunteers were given a subtoxic dose of 5 mg/kg 2,4-D orally, and urine and blood samples were collected to monitor 2,4-D levels. Based on pharmacokinetic analysis data, the plasma clearance half-life was determined to be 33 hours. /SRP: Unspecified salt or ester of 2,4-D/
Five male volunteers were administered a single oral dose of 5 mg/kg. Following this dose, 2,4-D was eliminated from plasma via first-order kinetics with a mean biological half-life of 11.7 hours. All subjects excreted 2,4-D in the urine with a mean biological half-life of 17.7 hours, primarily as free 2,4-D (83.3%), with a small amount excreted as 2,4-D conjugates (12.8%). /SRP: Unspecified salt or ester of 2,4-D/

Toxicity Summary
Identification and Uses: 2,4-Dichlorophenoxyacetic acid (2,4-D) is a herbicide. It is a white powder with a slightly phenolic odor. It is readily soluble in water, although the solubility of its ester products varies. It is used as a solid alkaline salt concentrate, a base-based water-soluble solution, or an ester-based emulsifiable concentrate; it can also be mixed with other herbicides. It is a component of the military defoliant Agent Orange. It is used to control broadleaf weeds in cereals, grain crops, roadsides, and farm buildings, and to increase latex yield in older rubber trees. Human Exposure and Use: 2,4-D can be absorbed through the gastrointestinal tract, by inhalation, and less frequently by intact skin. A study observed 220 men exposed to 0.5 to 22 years of 2,4-D at a manufacturing plant. Medical evaluation showed no difference compared to a control group of 4,600 men. Karyotype analysis was performed on 10 men in the exposure group. Results showed no impact on the structural integrity of lymphocyte chromosomes or the arrangement of genetic material. However, in vitro studies have shown that 2,4-D leads to increased chromatid and chromosome breakage, micronucleus numbers, and nucleus bud numbers, regardless of the presence of metabolic activators. The presence of S9 mixtures further increased the number of chromatid breakage and micronuclei in treated lymphocytes. In a plant producing amine salts and butyl esters, workers reported signs and symptoms including general weakness, fatigue, frequent headaches, and dizziness. Cases of arterial hypotension were also observed. Workers with long-term exposure to the herbicide may show signs of liver dysfunction. In two groups of agricultural workers, 250 and 45, respectively, reported excessive fatigue, epigastric pain, anorexia, occasional respiratory symptoms, and decreased taste. The reported cases of poisoning are mainly due to accidental or suicidal ingestion. Peripheral neuropathy and contact dermatitis have also been reported with poisoning. Animal studies: The substance can be absorbed through the gastrointestinal tract, inhalation, or intact skin. In vivo liver mitochondrial studies have shown that the herbicide can be uncoupled and oxidatively phosphorylated at low concentrations. Young female rats were given different doses of 2,4-D orally via gastric tube five times a week for four consecutive weeks. Animals in the high-dose group exhibited varying degrees of gastrointestinal irritation, mild hepatic turbidity and swelling, and decreased growth rate. The increased mortality rate in the high-dose group was due to severe gastrointestinal irritation. Cumulative effects may manifest as liver or kidney damage, but long-term exposure did not lead to definitive biochemical damage. Female rats were fed different concentrations of 2,4-D in their diet for up to two years. There was no significant difference in mortality between the experimental and control groups. Autopsies of animals surviving for two years revealed no difference in body weight and normal hematological parameters, but final examinations at 22 months showed a possible tendency towards giant cell, polychromatic, and hypochromatic lesions. The incidence of bile duct hyperplasia, mild hepatitis, and nephritis was slightly higher in the experimental group than in the control group. 2,4-D is not considered a carcinogen. A two-year rat feeding study found a slight increase in tumor incidence in female rats, but the raw data were insufficient to determine whether 2,4-D is carcinogenic. In some developmental experiments, rats, guinea pigs, hamsters, and mice treated with high doses of 2,4-D showed an increased incidence of mild skeletal deformities. 2,4-D is maternally toxic and embryo-lethal in rats, and can induce malformations of the urogenital system in rat fetuses. It is also teratogenic and embryotoxic in mice. Ecotoxicity studies: Crayfish were exposed to three sublethal concentrations of 2,4-D for 96 hours, then placed in a Y-maze system with fish gelatin food sources randomly placed in the left and right arms of the maze. Results showed that the crayfish's foraging ability was impaired. This reduced ability to find and ingest sufficient food may lead to decreased population weight and health in crayfish exposed to 2,4-D in their natural habitat. A mixture of 2,4-D and sodium formaldehyde ester may impair gill function and increase the crayfish's susceptibility to herbicide toxicity. 2,4-Dichlorophenoxyacetic acid is a strong oxidizing agent known to cause lipid peroxidation and the generation of free radicals, thereby modifying lipids and proteins. It is also known to inhibit glutathione S-transferase, leading to the depletion of ATP, NADPH, and glutathione (A3122, A3123). These effects can lead to cytotoxicity and apoptosis in metabolically active cells. Some endocrine effects of 2,4-D may be mediated through the following pathways: 2,4-D-mediated displacement of sex hormones from sex hormone-binding globulins, or 2,4-D-mediated blockade or blockade of OAT6 transporters, which are essential for the transport of functional organic ions and dicarboxylate salts (including estrone sulfate).

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Toxicity Data
LC50 (Rat)> 1,790 mg/m3
LD50: 1400 mg/kg (dermal, rabbit) (T14)
LD50: 469 mg/kg (oral, guinea pig)
LD50: 639 mg/kg (oral, rat) (L1982)
LD50: 138 mg/kg (oral, mouse) (L1982)

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Interactions
Toxicity of a widely used herbicide (Dicarmine D) This study investigated the toxicity of a herbicide with 72% sodium 2,4-D as the active ingredient, either alone or in combination with three heavy metals (copper sulfate, cadmium sulfate, and lead acetate) (simulating environmental heavy metal loads), using an injection method on chicken embryos. Treatments were administered on day 0 of incubation. Different concentrations of test materials were prepared into solutions and emulsions and injected into the air cavities of chicken embryos at a volume of 0.1 mL. Macroscopic assessment was performed on day 19 of incubation. The results showed that, compared with the control group, the toxicity of using herbicide formulations containing 72% 2,4-D alone was lower than that of using herbicides and heavy metals simultaneously. Compared with the single application of herbicide formulations containing 72% 2,4-D and heavy elements, the simultaneous application of cadmium and herbicides resulted in the highest embryonic mortality rate, while the incidence of developmental abnormalities was highest in the study of copper-pesticide interaction. /Dikamin D/
This study investigated the effects of a mixture of parathion (PA; 5 mg/kg), toxaphene (TOX; 50 mg/kg), and/or 2,4-dichlorophenoxyacetic acid (2,4-D; 50 mg/kg) on the mixed-function oxygenase (MFO) system of the liver in male ICR mice (21–24 g) by oral gavage once daily for 7 consecutive days. Overall, TOX and its mixtures induced the metabolism of aminopyrine (21-52%), aniline (58-72%), phenacetin (239-307%), pentobarbital (104-148%), and benzo[a]pyrene (143-304%) in 9000 g of liver supernatant, and increased the content of hepatocyte cytochrome P-450 (57-80%). Furthermore, TOX pretreatment effectively enhanced the biotransformation of paraoxon (PA) or paraoxon (PO) in the supernatant. 5 mM EDTA had no significant effect on this enhancement. Although TOX increased esterase activity in serum, liver homogenate, and supernatant by 31-158%, paraoxonase activity in these formulations was not affected. The TOX-induced increase in PA or PO metabolism was at least partially related to the MFO system, while paraoxonase was not significantly involved in this increase. These findings suggest that the PA+TOX mixture is less toxic than PA because TOX can increase the biotransformation of PA and PO, as well as the level of esterases, thus providing a non-critical enzyme for PO binding. Due to these properties of TOX, the PA+TOX+2,4-D mixture is also expected to be less toxic than PA. This study investigated the effects of prenatal exposure to a mixture of 2,4-dichlorophenoxyacetic acid (24-D)/2,4,5-trichlorophenoxyacetic acid on glutamate, γ-aminobutyric acid (GABA), protein, DNA, and RNA in rat brains. Pregnant Sprague Daley rats were orally administered a 1:1 mixture of 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid at doses of 0, 50, or 125 mg/kg/day between days 6 and 15 of gestation. This mixture is known to contain 0.0125 ppm of 2,3,7,8-tetrachlorodibenzo-p-oxin. On days 1, 15, or 22 postnatally, the brain tissue of newborn rats was isolated into the cerebrum, cerebellum, neocortex, and thalamus/hypothalamus, and the levels of glutamate, DNA, RNA, protein, and γ-aminobutyric acid (GABA) were measured. Prenatal exposure to the mixture had no effect on the concentrations of protein, DNA, and RNA in the brain regions, except for a decreased hypothalamic protein/DNA ratio in the 50 and 125 mg/kg dose groups on day 22 postnatally. Newborns exposed to 50 and 125 mg/kg 2,4-dichlorophenoxyacetic acid/2,4,5-trichlorophenoxyacetic acid on day 1 postnatally showed significantly reduced glutamate levels in the cerebrum and cerebellum, while no significant changes in glutamate levels were observed in offspring examined on days 15 and 22 postnatally. GABA was not significantly affected in any brain region at any time point. Myotonia is characterized by prolonged duration of skeletal muscle fiber contraction (delayed relaxation) accompanied by characteristic electromyographic findings. Calcium channel blockers are expected to reduce myotonia because they promote the relaxation of contracting skeletal muscles. This study aimed to evaluate the effect of the calcium channel blocker diltiazem on 2,4-dichlorophenoxyacetic acid (2,4-D)-induced myotonia. Rat diaphragms were exposed to a tissue bath containing 2.5 mM 2,4-D, and myotonia was quantified by recording the contraction time induced by supermaximal electrical stimulation. Diltiazem was added to the tissue bath at peak myotonia, and its effect on induced contraction was observed for 6 minutes. A concentration of 5 x 10⁻⁵ M was optimal, reducing contraction time by more than 90% within 3 minutes. For more complete data on interactions with 2,4-D (13 items in total), please visit the HSDB records page.

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Non-human toxicity values
Mouse LD50: 521 mg/kg
Rabbit skin LD50: 1400 mg/kg
Male Fischer-344 rat oral LD50: 443 mg/kg (95% confidence interval: 270-1103 mg/kg) (2,4-D acid soluble in corn oil).
The oral LD50 of undiluted 2,4-D/2,4,5-T (1:1) in 3-week-old chicks (male and female) is 4000 mg/kg (2700-5900 mg/kg). Acid equivalent not specified. /Excerpt from table/
For more complete data on non-human toxicity values for 2,4-D (of 8), please visit the HSDB records page.

2,4-Dichlorophenoxyacetic acid (2,4-D) is not naturally occurring in the environment. 2,4-D is the active ingredient in many herbicide products in the United States and around the world, used to control weeds on land and in water. 2,4-D is available in nine forms as a herbicide, typically sold in powder or liquid form. Sodium 2,4-D is a transparent brown to black liquid with a characteristic phenoxy odor. Its main hazard is environmental pollution. Immediate measures should be taken to limit its spread into the environment. It readily seeps into the soil, contaminating groundwater and nearby waterways. 2,4-Dichlorophenoxyacetic acid is an odorless white to brownish-red solid that sinks in water. (US Coast Guard, 1999) 2,4-D is a chlorophenoxyacetic acid in which the hydrogen atoms at positions 2 and 4 of the phenoxyacetic acid ring are replaced by chlorine atoms. It can be used as a synthetic auxin, defoliant, agrochemical, EC 1.1.1.25 (shikimate dehydrogenase) inhibitor, environmental pollutant, and phenoxy herbicide. It is a mixture of chlorophenoxyacetic acid and dichlorobenzene, and is the conjugate acid of (2,4-dichlorophenoxy)acetic acid ester. 2,4-Dichlorophenoxyacetic acid has been reported and data are available for its detection in Guanomyces polythrix, Nicotiana tabacum, and Phoma herbarum. 2,4-Dichlorophenoxyacetic acid (2,4-D) is a commonly used systemic herbicide for controlling broadleaf weeds. It is the most widely used herbicide in the world and the third most commonly used herbicide in North America. 2,4-D is also an important synthetic auxin, commonly used in plant research laboratories and as a supplement to plant cell culture media such as MS medium. (S685) 2,4-D can be formulated as emulsifiable concentrates, granules, soluble concentrates, and solid, water-dispersible granules and wettable powders. 2,4-D can be used alone, but is usually used in combination with dicamba, metolachlor, metolachlor-P, MCPA, and chlorpyrifos. 2,4-D is a component of Agent Orange, a herbicide widely used during the Vietnam War. Although 2,4-D constitutes 50% of Agent Orange, the health hazards of Agent Orange are related to dioxin contaminants generated during its production, not 2,4-D itself. On August 8, 2007, the U.S. Environmental Protection Agency issued a ruling stating that existing data do not support a link between human cancer and 2,4-D exposure.
A herbicide that is irritating to the eyes and gastrointestinal tract.

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Mechanism of Action
The widely used hormone-based herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) can block in vitro meiotic maturation, thus it is a potential environmental endocrine disruptor with early reproductive effects. To examine whether maturation inhibition depends on the endogenous maturation inhibitor protein kinase A (PKA), researchers microinjected a specific PKA inhibitor, PKI, into oocytes and then exposed them to 2,4-D. The results showed that the oocytes failed to mature, indicating that the effect of 2,4-D is independent of PKA activity and may act on downstream targets, such as Mos. This study investigated the de novo synthesis of Mos protein triggered by poly(A) elongation of mRNA. Radiolabeled Mos RNA in vitro transcripts were microinjected into oocytes and treated with progesterone and 2,4-D, respectively. RNA analysis showed that progesterone induced poly(A) elongation as expected, while 2,4-D did not induce poly(A) elongation. Locational studies showed that 2,4-D-activated MAPK is located in the cytoplasm, but its induction of Rsk2 phosphorylation and activation is weak. In addition to inhibiting the G2/M phase transition, 2,4-D also caused a sharp decline in H1 kinase activity in MII oocytes. Attempts to rescue the maturation process of oocytes transiently exposed to 2,4-D failed, indicating that 2,4-D induces irreversible dysfunction of meiotic signaling mechanisms. Chlorphenoxy herbicides are chemical analogs of the plant growth hormone auxin, causing uncontrolled and lethal growth in target plants. Daily gavage administration of 2,4-D (100-200 mg/kg body weight) to male Wistar rats induced hepatic peroxisome proliferation, decreased serum lipid levels, and increased the activity of hepatic carnitine acetyltransferase and catalase. Data suggest that the compound induces hypolipidemia by preferentially increasing hepatic lipid utilization. 2,4-Dichlorophenoxyacetic acid (2,4-D) is a hormone-based herbicide widely used worldwide due to its significant control effects on broadleaf and woody plants. This study used 2,4-D-specific antiserum for immunoblotting analysis to confirm the in vivo covalent binding of the phenoxy herbicide 2,4-D to a single 52 kDa protein in rat liver mitochondrial preparations. Furthermore, we used liver mitochondrial preparations exposed to 14C-UL-2,4-D to confirm in vitro that 2,4-D directly participates in the formation of this adduct. Radiolabeled proteins were separated by SDS-PAGE and then electroeluted, revealing only one 52 kDa labeled protein. After removing the outer membrane of mitochondria exposed to the radiolabeled herbicide, the observed specific activity indicated that the protein involved in the covalent interaction was located in the inner mitochondrial membrane. We believe that the covalent binding of phenoxy herbicide 2,4-D to a very specific single protein of 52 kD, observed in vitro and in vivo, may be related to known alterations in mitochondrial function.

C₈H₅CL₂NAO₃

243.02

241.951

2702-72-9

2,4-D;94-75-7

1486

White to yellow crystalline powder /SRP: yellow color is phenolic impurities/
Colorless powder
White to yellow, crystalline ... powder
Crystals from benzene

345.6ºC at 760 mmHg

215°C

162.8ºC

2.31E-05mmHg at 25°C

1.122

1

3

3

13

186

0

ClC1C([H])=C(C([H])=C([H])C=1OC([H])([H])C(=O)O[H])Cl

RFOHRSIAXQACDB-UHFFFAOYSA-M

InChI=1S/C8H6Cl2O3.Na/c9-5-1-2-7(6(10)3-5)13-4-8(11)12;/h1-3H,4H2,(H,11,12);/q;+1/p-1

sodium;2-(2,4-dichlorophenoxy)acetate

2,4D sodium salt; 2,4 D sodium salt

2934.99.9001

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.

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Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)
*Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution.
Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)
Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil)
Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals).

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Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)]
*Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.
Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline)
Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline)
Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline)
Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline
Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil)
Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline)

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Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium)
Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose
Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals).

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Oral Formulation 3: Dissolved in PEG400
Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose
Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose
Oral Formulation 6: Mixing with food powders

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Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.

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 (Please use freshly prepared in vivo formulations for optimal results.)