| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
15-Azido-pentadecanoic acid does not have a specific biological target as it is a chemical probe for studying protein palmitoylation. The compound serves as a fatty acid analog that can be metabolically incorporated into palmitoylated proteins, where the azide group enables subsequent detection and identification via click chemistry.
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| ln Vitro |
In vitro activity of 15-azido-pentadecanoic acid is demonstrated by its utility as a metabolic labeling probe for palmitoylated proteins. The compound is incorporated into proteins via the palmitoylation pathway, and the azide group enables detection and characterization through click chemistry with alkyne-containing tags.
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| ln Vivo |
In vivo activity of 15-azido-pentadecanoic acid is observed in its use for labeling palmitoylated proteins in living cells or organisms. The compound is metabolically incorporated into proteins via the palmitoylation pathway, enabling the identification and characterization of palmitoylated proteins in biological systems.
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| Enzyme Assay |
In vitro enzyme assays for 15-azido-pentadecanoic acid are not typically performed, as the compound is used as a metabolic labeling probe rather than as an enzyme inhibitor. Its incorporation into proteins can be assessed by detecting the azide tag using click chemistry with fluorescent or biotinylated alkyne probes.
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| Cell Assay |
In vitro cellular assays for 15-azido-pentadecanoic acid involve treating cells with the compound to allow metabolic incorporation into palmitoylated proteins. Labeled proteins are then detected and identified by click chemistry conjugation with alkyne-containing tags, followed by gel electrophoresis, Western blotting, or mass spectrometry.
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| Animal Protocol |
In vivo animal experiments for 15-azido-pentadecanoic acid would typically involve administering the compound to animals to label palmitoylated proteins in tissues. Labeled proteins can be detected and characterized using click chemistry with alkyne-containing tags in tissue lysates.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 15-azido-pentadecanoic acid are limited. As a fatty acid analog, its absorption, distribution, metabolism, and excretion properties would be influenced by its lipid nature. The compound is a solid with a purity of ≥98%. Further pharmacokinetic studies are needed.
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| Toxicity/Toxicokinetics |
Toxicological data for 15-azido-pentadecanoic acid are limited. As a research chemical, comprehensive toxicological studies have not been reported. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
15-Azido-pentadecanoic acid (Azido Palmitic Acid) (CAS#: 118162-46-2) has the molecular formula C15H29N3O2 and a molecular weight of 283.41. The compound is a click chemistry reagent used for identifying and characterizing palmitoylated proteins.
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| Molecular Formula |
C15H29N3O2
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|---|---|
| Molecular Weight |
283.41
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| Exact Mass |
283.226
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| CAS # |
118162-46-2
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| PubChem CID |
14006108
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| Appearance |
White to off-white solid powder
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| LogP |
4.905
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
15
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| Heavy Atom Count |
20
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| Complexity |
276
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CCCCCCCN=[N+]=[N-])CCCCCCC(=O)O
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| InChi Key |
PYGQAGHMXCCROO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H29N3O2/c16-18-17-14-12-10-8-6-4-2-1-3-5-7-9-11-13-15(19)20/h1-14H2,(H,19,20)
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| Chemical Name |
15-azidopentadecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (352.85 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5285 mL | 17.6423 mL | 35.2846 mL | |
| 5 mM | 0.7057 mL | 3.5285 mL | 7.0569 mL | |
| 10 mM | 0.3528 mL | 1.7642 mL | 3.5285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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