| Size | Price | Stock | Qty |
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| 10mg |
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| Targets |
The primary target of 1,4-Dichloro 5-carboxytetramethylrhodamine is not a biological receptor or enzyme, but rather the biomolecule to which it is conjugated for detection purposes. As a fluorescent labeling reagent, the compound is used to label proteins, antibodies, nucleic acids, and other biomolecules for fluorescence-based detection and imaging applications. The carboxy group on the compound can be activated to form reactive esters (e.g., NHS esters) that react with primary amines on biomolecules to form stable amide bonds. The labeled biomolecules can then be detected by fluorescence microscopy, flow cytometry, or other fluorescence-based methods.
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| ln Vitro |
In vitro, 1,4-Dichloro 5-carboxytetramethylrhodamine is used as a fluorescent labeling reagent for various biochemical applications. The compound has excitation/emission wavelengths of 541/568 nm, making it suitable for use in fluorescence microscopy, flow cytometry, and other fluorescence-based assays. The compound is used to label proteins, antibodies, nucleic acids, and other biomolecules. The labeled biomolecules retain their biological activity and can be used for detection and imaging purposes. The compound's fluorescence properties allow for sensitive detection of target molecules in complex biological samples.
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| ln Vivo |
In vivo studies of 1,4-Dichloro 5-carboxytetramethylrhodamine are limited, as the compound is primarily used as an in vitro research reagent for labeling and detection. However, fluorescent dyes like this compound may be used in animal models for imaging applications, such as tracking cells or biomolecules in vivo. In such studies, labeled biomolecules are administered to animals, and their distribution and fate are monitored by fluorescence imaging. The compound's fluorescence properties enable sensitive detection in biological samples. No extensive in vivo pharmacological or toxicological studies have been reported.
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| Enzyme Assay |
For fluorescence characterization, 1,4-Dichloro 5-carboxytetramethylrhodamine is dissolved in an appropriate solvent (e.g., DMSO, methanol, or aqueous buffer) and its absorption and emission spectra are measured using a UV-Vis spectrophotometer and fluorescence spectrometer, respectively. Excitation and emission maxima are determined (Ex=541 nm, Em=568 nm). For labeling reactions, the compound is activated by converting the carboxy group to an NHS ester using EDC and NHS. The activated ester is then reacted with the target biomolecule (e.g., protein, antibody, or nucleic acid) in a buffer at pH 7.0-8.5 for 1-2 hours at room temperature. The labeled product is purified by dialysis, size exclusion chromatography, or other methods. The labeling efficiency is assessed by measuring the absorbance or fluorescence of the labeled product.
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| Cell Assay |
For cellular studies, 1,4-Dichloro 5-carboxytetramethylrhodamine-labeled antibodies or other probes are used to stain cells for fluorescence microscopy or flow cytometry. Cells are cultured and fixed, then incubated with the labeled probe. After washing, cells are analyzed by fluorescence microscopy or flow cytometry using appropriate filter sets for excitation at 541 nm and emission at 568 nm. The labeled probes can be used to detect specific antigens, proteins, or other targets in cells. For live-cell imaging, cells may be incubated with labeled probes and imaged in real-time.
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| Animal Protocol |
For in vivo imaging studies, 1,4-Dichloro 5-carboxytetramethylrhodamine-labeled biomolecules are administered to animals via intravenous or intraperitoneal injection. The distribution and fate of the labeled molecules are monitored using an in vivo imaging system equipped with appropriate excitation and emission filters. Tissues and organs may be collected at various time points for ex vivo fluorescence imaging or homogenization for fluorometric analysis. However, such studies are not typical for this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 1,4-Dichloro 5-carboxytetramethylrhodamine are not available, as the compound is used as a research reagent rather than a therapeutic agent. If used in vivo, the compound would be expected to have distribution to various tissues. However, no dedicated ADME studies have been reported. The compound is primarily used in vitro and is not intended for in vivo pharmacokinetic characterization.
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| Toxicity/Toxicokinetics |
Toxicological data for 1,4-Dichloro 5-carboxytetramethylrhodamine are limited, as the compound is used as a research reagent. The compound is generally considered to have low toxicity at the concentrations used for labeling and staining applications. No acute toxicity, mutagenicity, or carcinogenicity data have been reported. As with all chemical reagents, appropriate safety precautions should be taken when handling 1,4-Dichloro 5-carboxytetramethylrhodamine, including the use of personal protective equipment and work in a well-ventilated area.
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| Additional Infomation |
1,4-Dichloro 5-carboxytetramethylrhodamine (CAS 198546-47-3) is a rhodamine-class fluorescent dye with excitation/emission of 541/568 nm. It has a molecular formula of C₂₅H₂₀Cl₂N₂O₅ and a molecular weight of 499.34. The compound is a fluorescent labeling reagent used to label proteins, antibodies, and nucleic acids. It is a xanthylium-based compound with dimethylamino groups. The labeled biomolecules can be detected by fluorescence microscopy or flow cytometry. The compound is strictly for research use only.
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| Molecular Formula |
C25H20N2O5CL2
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|---|---|
| Molecular Weight |
499.343
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| Exact Mass |
498.074
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| CAS # |
198546-47-3
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| PubChem CID |
91827773
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| Appearance |
Brown to black solid powder
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| LogP |
4.4
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
34
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| Complexity |
948
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN(C)C1=CC2=C(C=C1)C(=C3C=CC(=[N+](C)C)C=C3O2)C4=C(C=C(C(=C4C(=O)O)Cl)C(=O)[O-])Cl
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| InChi Key |
KWFRWLVSVLQCPR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H20Cl2N2O5/c1-28(2)12-5-7-14-18(9-12)34-19-10-13(29(3)4)6-8-15(19)20(14)21-17(26)11-16(24(30)31)23(27)22(21)25(32)33/h5-11H,1-4H3,(H-,30,31,32,33)
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| Chemical Name |
3-carboxy-2,5-dichloro-4-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0026 mL | 10.0132 mL | 20.0264 mL | |
| 5 mM | 0.4005 mL | 2.0026 mL | 4.0053 mL | |
| 10 mM | 0.2003 mL | 1.0013 mL | 2.0026 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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