| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
- 1,4-b-D-Xylopentaose binds to carbohydrate binding modules (CBMs), specifically CBM64 and CBM35, which are associated with xyloglucan-degrading enzymesThe binding affinity was characterized by a dissociation constant (Kd) of ~12 μM for CBM64 (measured via isothermal titration calorimetry, ITC) [1]
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| ln Vitro |
- 1,4-b-D-Xylopentaose formed stable complexes with CBM64 and CBM35, as confirmed by X-ray crystallography. The complex structure revealed that 1,4-b-D-Xylopentaose interacted with CBM64 through polar contacts (hydrogen bonds) between its hydroxyl groups and amino acid residues (e.g., Asn42, Gln68) of CBM64, and via CH-Π interactions between its pyranose rings and aromatic residues (e.g., Trp28) of CBM64 [1]
- Via isothermal titration calorimetry (ITC), 1,4-b-D-Xylopentaose showed specific binding to CBM64 with a stoichiometry (n) of ~1:1 (1 molecule of 1,4-b-D-Xylopentaose per CBM64 monomer) and a dissociation constant (Kd) of ~12 μM. No significant binding was detected between 1,4-b-D-Xylopentaose and CBM35 in ITC assays [1] |
| Enzyme Assay |
- For ITC-based binding assay (CBM64 and 1,4-b-D-Xylopentaose): CBM64 protein was purified and dialyzed against a buffer containing 20 mM Tris-HCl (pH 7.5) and 150 mM NaCl. 1,4-b-D-Xylopentaose was dissolved in the same buffer to a concentration of 1 mM. The ITC experiment was conducted at 25°C, with 1,4-b-D-Xylopentaose injected stepwise (2 μL per injection) into the sample cell containing CBM64 (20 μM). The heat change per injection was recorded, and the data were fitted to a one-site binding model to calculate the dissociation constant (Kd), stoichiometry (n), and binding enthalpy (ΔH) [1]
- For X-ray crystallography of CBM64-1,4-b-D-Xylopentaose complex: CBM64 protein was concentrated to 10 mg/mL in a buffer with 20 mM Tris-HCl (pH 7.5) and 100 mM NaCl. 1,4-b-D-Xylopentaose was added to the protein solution at a molar ratio of 1.5:1 (ligand:protein), and the mixture was incubated on ice for 1 h. Crystals were grown via the hanging-drop vapor diffusion method using a reservoir solution containing 20% polyethylene glycol 3350 and 0.2 M sodium citrate (pH 6.0). Diffraction data were collected at a synchrotron radiation source, and the complex structure was solved by molecular replacement using the unliganded CBM64 structure as a template [1] |
| References | |
| Additional Infomation |
1,4-β-D-xylopentanose is a linear pentameric xylosaccharide oligosaccharide (β-1,4-linked D-xylopyranose unit) that can be used as a model ligand to study the substrate specificity of carbohydrate binding modules (CBMs) involved in the degradation of plant cell wall polysaccharides [1]. The binding of 1,4-β-D-xylopentanose to CBM64 provides structural information for CBM to recognize xylosaccharide oligosaccharides: the polar contacts and CH-π interactions found in the complex are conserved features of CBM-xyloside binding, which helps to explain the role of CBM in targeting xyloglucan-degrading enzymes to their substrates [1].
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| Molecular Formula |
C25H42O21
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|---|---|
| Molecular Weight |
678.5884
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| Exact Mass |
678.222
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| CAS # |
49694-20-4
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~147.36 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4736 mL | 7.3682 mL | 14.7364 mL | |
| 5 mM | 0.2947 mL | 1.4736 mL | 2.9473 mL | |
| 10 mM | 0.1474 mL | 0.7368 mL | 1.4736 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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