| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
10-Methyl-9-(phenoxycarbonyl)acridinium does not have a specific biological target. It is a chemiluminescent probe that reacts with hydrogen peroxide and reactive oxygen species to produce light. The compound's mechanism of action is based on its chemical reactivity rather than engagement with a pharmacological target. Upon reaction with hydrogen peroxide under neutral conditions, the acridinium ester undergoes chemiluminescence, producing a detectable signal.
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| ln Vitro |
In vitro, 10-Methyl-9-(phenoxycarbonyl)acridinium is used as a chemiluminescent reagent for the quantification of hydrogen peroxide. It has been used in chemiluminescence reactions to detect and quantify ROS. The compound produces chemiluminescence under neutral conditions, making it suitable for biological assays. Specific activity data, such as EC50 values, are not applicable as it is a detection reagent rather than a pharmacologically active compound.
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| ln Vivo |
In vivo applications of 10-Methyl-9-(phenoxycarbonyl)acridinium are not typical, as it is primarily used in vitro as a chemiluminescent probe. It is not administered to living animals for therapeutic or diagnostic purposes. Its use is confined to laboratory research settings for detecting hydrogen peroxide and ROS in biological samples.
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| Enzyme Assay |
A cell-free assay for 10-Methyl-9-(phenoxycarbonyl)acridinium involves measuring its chemiluminescence upon reaction with hydrogen peroxide. In these assays, the compound is incubated with hydrogen peroxide under neutral conditions, and the resulting chemiluminescence is measured using a luminometer. The intensity of the signal is proportional to the concentration of hydrogen peroxide, allowing for quantification.
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| Cell Assay |
Cellular experiments with 10-Methyl-9-(phenoxycarbonyl)acridinium typically involve using it as a probe to detect ROS production in cells. Cells are incubated with the compound, and upon production of ROS or hydrogen peroxide, chemiluminescence is generated and measured. However, specific protocols are not detailed in the available sources. The compound is not stable in solution and should be used freshly prepared.
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| Animal Protocol |
In vivo animal experiments for 10-Methyl-9-(phenoxycarbonyl)acridinium are not applicable, as it is a chemiluminescent probe used in vitro.
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| ADME/Pharmacokinetics |
10-Methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate has a molecular formula of C21H16FNO5S and a molecular weight of 413.42. It is soluble in DMSO at approximately 35.71 mg/mL. The compound should be stored in a sealed and protected environment, avoiding exposure to moisture and light. It is not stable in solution and should be used freshly prepared. For powder storage, it can be kept at -20°C for 3 years or at 4°C for 2 years.
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| Toxicity/Toxicokinetics |
Toxicity data for 10-Methyl-9-(phenoxycarbonyl)acridinium are not provided in the available sources. As a chemiluminescent probe, it is generally considered to have low toxicity for its intended research applications. However, specific toxicological information is not available. The compound is intended for research use only and not for human consumption.
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| References | |
| Additional Infomation |
10-Methyl-9-(phenoxycarbonyl)acridinium fluorosulfonate (CAS: 149300-54-9) is a phenyl ester of acridinium ester that produces chemiluminescence under neutral conditions. It is also known as PMAC. The compound is used for the quantification of hydrogen peroxide and as a sensitive tool for detection of reactive oxygen species. Its molecular formula is C21H16FNO5S and its molecular weight is 413.42. It is not a therapeutic drug and is strictly for research purposes.
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| Molecular Formula |
C21H16NO2+.O3FS-
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|---|---|
| Molecular Weight |
413.41884
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| Exact Mass |
413.073
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| CAS # |
149300-54-9
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| PubChem CID |
16218376
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
4.533
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
29
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| Complexity |
500
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| Defined Atom Stereocenter Count |
0
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. (2). This product is not stable in solution, please use freshly prepared working solution for optimal results. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~35.71 mg/mL (~86.38 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4188 mL | 12.0942 mL | 24.1885 mL | |
| 5 mM | 0.4838 mL | 2.4188 mL | 4.8377 mL | |
| 10 mM | 0.2419 mL | 1.2094 mL | 2.4188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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