| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
As a deuterium-labeled stable isotope, 1-Aminocyclopropane-1-carboxylic acid-d4 does not exhibit a specific pharmacological target in mammalian systems. However, its unlabeled counterpart, ACC, serves as the key endogenous precursor in the ethylene biosynthesis pathway in plants, where it is converted to ethylene by the enzyme ACC oxidase. The labeled compound is therefore utilized as an analytical tracer to study ACC metabolism and its role in plant physiology and development.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
1-Aminocyclopropane-1-carboxylic acid-d4 does not exhibit intrinsic pharmacological activity in mammalian systems. Its primary in vitro application is as an internal standard for the quantification of ACC in various biological matrices using mass spectrometry. Stable heavy isotopes such as deuterium have been incorporated into drug molecules and metabolites as tracers for quantitation during analytical method development. The compound serves as a quantitative tool for studying ACC levels in plant tissues and related biological samples. |
| ln Vivo |
In vivo, 1-Aminocyclopropane-1-carboxylic acid-d4 is used as a tracer in metabolic studies to track the absorption, distribution, metabolism, and excretion of ACC in both plant and animal systems. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry. Deuteration may affect the pharmacokinetic and metabolic properties of the compound, which is an important consideration in tracer studies.
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| Enzyme Assay |
As an analytical standard, 1-Aminocyclopropane-1-carboxylic acid-d4 is used in enzyme assays and metabolic flux studies. Typical protocols involve spiking the labeled compound into samples prior to extraction and analysis by GC-MS or LC-MS. The compound serves as an internal standard to correct for matrix effects and instrument variability. The labeled compound co-elutes with the unlabeled analyte but is detected at a different mass-to-charge ratio.
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| Cell Assay |
In vitro cell culture experiments using 1-Aminocyclopropane-1-carboxylic acid-d4 involve supplementing growth media with the labeled compound at defined concentrations. Plant or mammalian cells are cultured for specified periods, after which the metabolism of the compound is analyzed by mass spectrometry. The compound can also be used to study ACC transport and metabolism in various cell types. Detailed protocols are typically optimized based on the specific experimental design.
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| Animal Protocol |
In vivo animal studies using 1-Aminocyclopropane-1-carboxylic acid-d4 typically involve administering the compound to rodents via oral gavage or intravenous injection. Blood, urine, and tissue samples are collected at various time points to track the distribution and metabolism of ACC. The deuterium label allows for precise quantification using mass spectrometry. Detailed protocols are generally established based on standard pharmacokinetic study designs.
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| ADME/Pharmacokinetics |
1-Aminocyclopropane-1-carboxylic acid-d4 exhibits pharmacokinetic properties similar to those of unlabeled ACC. As an amino acid derivative, it is absorbed from the gastrointestinal tract via specific amino acid transporters and distributed throughout the body. It is metabolized primarily in the liver and excreted in urine. The deuterium label provides a distinct mass shift for analytical detection without significantly altering the compound's physicochemical properties.
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| Toxicity/Toxicokinetics |
1-Aminocyclopropane-1-carboxylic acid-d4 is considered safe for research use at typical concentrations. The compound is non-hazardous for transport and stable if stored under recommended conditions. After extended storage, the compound should be re-analyzed for chemical purity before use. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References | |
| Additional Infomation |
1-Aminocyclopropane-1-carboxylic acid-d4 has a molecular weight of 105.13 and typically appears as a white to off-white solid. The compound is stable isotope-labeled with an enrichment of 98 atom% D. It is primarily used as an internal standard for mass spectrometry-based quantification of ACC in plant science and metabolic research. The compound should be stored under recommended conditions and re-analyzed for chemical purity after three years.
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| Molecular Formula |
C4H7NO2
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|---|---|
| Molecular Weight |
105.128527879715
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| Exact Mass |
105.073
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| CAS # |
84392-07-4
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| Related CAS # |
1-Aminocyclopropane-1-carboxylic acid;22059-21-8
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| PubChem CID |
12510847
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| Appearance |
White to off-white solid powder
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| LogP |
0.262
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
7
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| Complexity |
106
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C1(C(C1(C(=O)O)N)([2H])[2H])[2H]
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| InChi Key |
PAJPWUMXBYXFCZ-LNLMKGTHSA-N
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| InChi Code |
InChI=1S/C4H7NO2/c5-4(1-2-4)3(6)7/h1-2,5H2,(H,6,7)/i1D2,2D2
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| Chemical Name |
1-amino-2,2,3,3-tetradeuteriocyclopropane-1-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ≥ 100 mg/mL (~951.20 mM)
DMSO :< 1 mg/mL |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.5120 mL | 47.5602 mL | 95.1203 mL | |
| 5 mM | 1.9024 mL | 9.5120 mL | 19.0241 mL | |
| 10 mM | 0.9512 mL | 4.7560 mL | 9.5120 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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