| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The primary target of 1-methyl-2-pentyl-4(1H)-quinolinone is Plasmodium falciparum type II NADH dehydrogenase (PfNDH2). The compound is a specific inhibitor of PfNDH2. By inhibiting this enzyme, the compound disrupts the parasite's energy metabolism. 1-Methyl-2-pentyl-4(1H)-quinolinone significantly inhibits malaria parasite development in the mosquito stage. The compound has low toxicity.
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|---|---|
| ln Vitro |
In vitro studies have demonstrated the biological activities of 1-methyl-2-pentyl-4(1H)-quinolinone. The compound has antibacterial activity. It has anticancer bioactivity. The compound inhibits PfNDH2. These activities support its potential for further investigation.
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| ln Vivo |
In vivo studies have shown that 1-methyl-2-pentyl-4(1H)-quinolinone significantly inhibits the development of malaria parasites in the mosquito stage. The compound has low toxicity. It has potential for blocking malaria transmission. Specific in vivo data have been reported in the literature.
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| Enzyme Assay |
For PfNDH2 inhibition assays, recombinant PfNDH2 enzyme is incubated with a substrate in the presence of 1-methyl-2-pentyl-4(1H)-quinolinone. Enzyme activity is measured, and IC₅₀ values are calculated. For antibacterial assays, standard broth microdilution methods are used. For anticancer assays, cancer cell lines are treated with the compound.
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| Cell Assay |
For in vitro cell-based studies, cancer cell lines or bacterial cells are cultured in appropriate media and treated with serial dilutions of 1-methyl-2-pentyl-4(1H)-quinolinone. Cell viability is assessed using MTT or other assays. For antimalarial studies, P. falciparum parasites are cultured and treated with the compound.
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| Animal Protocol |
In vivo animal studies for 1-methyl-2-pentyl-4(1H)-quinolinone have been conducted in models of malaria transmission. The compound's effects on mosquito-stage parasite development have been evaluated. Standard protocols for in vivo efficacy studies are described in the literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 1-methyl-2-pentyl-4(1H)-quinolinone have not been extensively characterized. As a quinolone alkaloid with a molecular weight of 229.32 g/mol, it is expected to have reasonable membrane permeability. The compound has low toxicity. Specific PK parameters have not been reported.
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| Toxicity/Toxicokinetics |
Toxicological data for 1-methyl-2-pentyl-4(1H)-quinolinone indicate that the compound has low toxicity. As a natural alkaloid, it may have a favorable safety profile. However, systematic toxicological studies have not been extensively published. The compound is for research use only.
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| References | |
| Additional Infomation |
1-Methyl-2-pentyl-4(1H)-quinolinone is a research compound used in studies of quinolone alkaloids and their biological activities. It is a specific PfNDH2 inhibitor with potential for blocking malaria transmission. The compound also has antibacterial and anticancer activities. No clinical trials or therapeutic applications have been reported.
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| Molecular Formula |
C15H19NO
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|---|---|
| Molecular Weight |
229.32
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| Exact Mass |
229.146
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| CAS # |
22048-98-2
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| PubChem CID |
21709636
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
332.1±42.0 °C at 760 mmHg
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| Flash Point |
115.3±17.3 °C
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| Vapour Pressure |
0.0±0.7 mmHg at 25°C
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| Index of Refraction |
1.539
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| Source |
Originated from plants: other families
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| LogP |
3.97
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
17
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| Complexity |
308
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C([H])=C(C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H])N(C([H])([H])[H])C2=C([H])C([H])=C([H])C([H])=C21
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| InChi Key |
CHULATXGEVFYAJ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H19NO/c1-3-4-5-8-12-11-15(17)13-9-6-7-10-14(13)16(12)2/h6-7,9-11H,3-5,8H2,1-2H3
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| Chemical Name |
1-methyl-2-pentylquinolin-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~218.0 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.90 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.90 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (10.90 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3607 mL | 21.8036 mL | 43.6072 mL | |
| 5 mM | 0.8721 mL | 4.3607 mL | 8.7214 mL | |
| 10 mM | 0.4361 mL | 2.1804 mL | 4.3607 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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