| Size | Price | Stock | Qty |
|---|---|---|---|
| 250mg |
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| Other Sizes |
| Targets |
1,2-Distearoyl-sn-glycerol does not have a specific biological target but functions as an analytical standard and a lipid molecule. As a 1,2-diacyl-sn-glycerol, it is a structural analog of diacylglycerol (DAG), a key lipid second messenger involved in protein kinase C (PKC) signaling pathways. The compound is used as an internal standard in lipid analysis. It is also used in studies of platelet-derived growth factor (PDGF) influence on diacylglycerol phosphorylation.
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| ln Vitro |
1,2-Distearoyl-sn-glycerol is used as an internal standard for the isolation and identification of 1,2-diacyl-sn-glycerol (DAG) molecular species. It is a 1,2-diacyl-sn-glycerol and a diacylglycerol 36:0. The compound is used in the study of the influence of platelet-derived growth factor (PDGF) on diacylglycerol phosphorylation in Swiss 3T3 cells. Its biological activity is primarily as a reference standard.
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| ln Vivo |
In vivo, 1,2-distearoyl-sn-glycerol is a lipid molecule that can be used in studies of lipid metabolism and signaling. It is an internal standard for lipid analysis and is not used as a therapeutic agent. The compound may be incorporated into cells and metabolized similarly to endogenous diacylglycerols. Specific in vivo efficacy studies are not applicable, as the compound is used as an analytical standard.
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| Enzyme Assay |
In vitro assays for 1,2-distearoyl-sn-glycerol typically involve its use as an internal standard in lipidomics studies. The compound is added to lipid extracts at known concentrations before analysis by HPLC, TLC, or mass spectrometry. The compound is dissolved in organic solvents such as THF or chloroform. Standard protocols involve lipid extraction, separation by chromatography, and detection by mass spectrometry.
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| Cell Assay |
In vitro cellular assays for 1,2-distearoyl-sn-glycerol typically involve treating cells with the compound to study lipid metabolism and signaling. Cells are incubated with the compound at concentrations ranging from 1 to 100 µM for various time periods. Cellular lipids are extracted and analyzed by HPLC or mass spectrometry. The compound's effects on PKC activation or other signaling pathways can be assessed. The compound is dissolved in DMSO or ethanol and diluted in cell culture medium.
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| Animal Protocol |
In vivo animal studies for 1,2-distearoyl-sn-glycerol are not typically performed, as the compound is used as an analytical standard. The compound may be used in metabolic studies to trace lipid metabolism. The compound is administered to animals, and tissue samples are collected for lipid analysis. The compound is not intended for human therapeutic use.
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| ADME/Pharmacokinetics |
1,2-Distearoyl-sn-glycerol has a molecular weight of 625.02 g/mol and formula C39H76O5. It typically exists as a solid at room temperature. Recommended storage is powder at -20°C for 3 years. The compound is soluble in THF (50 mg/mL). It is a 1,2-diacyl-sn-glycerol and a diacylglycerol 36:0. Detailed PK parameters are not applicable for this analytical standard.
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| Toxicity/Toxicokinetics |
1,2-Distearoyl-sn-glycerol is intended for research use only and is not approved for human therapeutic applications. As a lipid molecule, it is generally considered to have low toxicity. Appropriate safety precautions should be taken when handling. The compound is used as an internal standard for lipid analysis.
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| Additional Infomation |
1,2-Dioctanoyl-sn-glycerol is a 1,2-diacyl-sn-glycerol in which both acyl groups are octadecanoyl groups. It is a 1,2-diacyl-sn-glycerol, and also a diacylglycerol 36:0. Functionally, it is related to octadecanoic acid. 1,2-Dioctanoyl-sn-glycerol is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). DG(18:0/18:0/0:0) is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). 1,2-Distearateyl-sn-glycerol has also been reported in Sciadopitys verticillata, and relevant data are available.
1,2-Distearoyl-sn-glycerol (CAS 10567-21-2) is a 1,2-diacyl-sn-glycerol in which both acyl groups are octadecanoyl groups. It has a molecular weight of 625.02 g/mol and formula C39H76O5. The compound is used as an internal standard for the isolation and identification of 1,2-diacyl-sn-glycerol (DAG) molecular species. It is also known as DSG and 1,2-dioctadecanoyl-sn-glycerol. The compound is not approved for clinical use. |
| Molecular Formula |
C39H76O5
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|---|---|
| Molecular Weight |
625.01774
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| Exact Mass |
624.569
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| CAS # |
10567-21-2
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| PubChem CID |
9543705
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| Appearance |
White to light yellow solid powder
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| Density |
0.9±0.1 g/cm3
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| Boiling Point |
662.3±22.0 °C at 760 mmHg
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| Flash Point |
181.8±15.8 °C
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| Vapour Pressure |
0.0±4.6 mmHg at 25°C
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| Index of Refraction |
1.467
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| LogP |
16.47
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
38
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| Heavy Atom Count |
44
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| Complexity |
593
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CCCCCCCCCCCCCCCCCC(=O)OCC(CO)OC(=O)CCCCCCCCCCCCCCCCC
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| InChi Key |
UHUSDOQQWJGJQS-QNGWXLTQSA-N
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| InChi Code |
InChI=1S/C39H76O5/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-31-33-38(41)43-36-37(35-40)44-39(42)34-32-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h37,40H,3-36H2,1-2H3/t37-/m0/s1
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| Chemical Name |
[(2S)-3-hydroxy-2-octadecanoyloxypropyl] octadecanoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
THF : 50 mg/mL (~80.00 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5999 mL | 7.9997 mL | 15.9995 mL | |
| 5 mM | 0.3200 mL | 1.5999 mL | 3.1999 mL | |
| 10 mM | 0.1600 mL | 0.8000 mL | 1.5999 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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