| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt does not have a specific pharmacological target as it is a phospholipid used for membrane studies rather than a drug. Its function is to form lipid bilayers and vesicles that mimic biological membranes for research applications. The negatively charged phosphate group provides the lipid with anionic properties, making it useful for studying membrane interactions with charged molecules. DOPA is used to study the structure and function of membrane proteins, including ion channels, transporters, and receptors. The lipid's oleic acid chains provide fluidity to the bilayer, mimicking the properties of biological membranes. DOPA is also used in drug delivery research as a component of liposomal formulations. The compound's ability to form stable bilayers makes it suitable for various biophysical techniques including surface plasmon resonance, Langmuir-Blodgett films, and electrophysiology.
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| ln Vitro |
1,2-Dioleoyl-sn-glycero-3-sodium phosphate (DOPA) is capable of being utilized to create Zol-Ca composite nanoparticle molecules, which will increase Zol's hydrophobicity and improve its distribution in mice tumor models [1]. Hi, When used in conjunction with the fluorescent dye calcium phosphate, 2-Dioleoyl-sn-glycero-3-phosphate sodium salt (DOPA) can improve the dye's capacity to bind to bacterial surfaces, transfer fluorescent groups, and activate S. aureus biofilms, hence improving biofilm staining [2].
In vitro, 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt is used to prepare liposomes and lipid bilayers for studying membrane proteins and lipid interactions. The lipid forms fluid bilayers at physiological temperatures due to the unsaturated oleic acid chains. DOPA is used to create supported lipid bilayers on solid surfaces for biophysical studies. The negatively charged headgroup allows for the study of electrostatic interactions with proteins and other molecules. DOPA liposomes are used to encapsulate drugs or other compounds for delivery studies. The lipid is also used in studies of membrane fusion, as phosphatidic acid is involved in membrane fusion processes. In enzyme assays, DOPA is used as a substrate for phospholipases and other lipid-modifying enzymes. The compound's properties make it suitable for studying the structure and function of membrane-associated proteins. |
| ln Vivo |
In vivo, 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt is not used as a therapeutic agent but as a research tool for studying lipid metabolism and membrane biology. The compound may be used in animal studies as a component of liposomal drug delivery systems. DOPA liposomes can be administered intravenously, intraperitoneally, or via other routes to deliver encapsulated drugs. The lipid's biocompatibility makes it suitable for in vivo applications. In studies of lipid metabolism, DOPA may be administered to animals to study its metabolism and effects on lipid homeostasis. The compound is also used in studies of phosphatidic acid signaling, as phosphatidic acid is a lipid second messenger involved in various cellular processes. The compound is not approved for clinical use and is intended for research applications only.
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| Enzyme Assay |
In vitro experiments with 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt typically involve preparing lipid films or liposomes. The lipid is dissolved in chloroform or other organic solvent, and the solvent is evaporated to form a thin lipid film. The film is hydrated with aqueous buffer to form multilamellar vesicles, which can be further processed by extrusion to form unilamellar vesicles. For electrophysiological measurements, DOPA bilayers are formed across an aperture in a partition separating two aqueous compartments. For supported bilayers, DOPA vesicles are deposited onto a solid support such as glass or mica. The lipid's properties make it suitable for various biophysical techniques. The compound is soluble in chloroform and other organic solvents. For long-term storage, the lipid is kept at -20°C and protected from light.
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| Cell Assay |
In vitro cell-based assays using 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt are typically performed using liposomes or lipid nanoparticles as delivery vehicles or membrane models. DOPA liposomes can be loaded with drugs or other compounds and used to study cellular uptake and drug delivery. Cells are incubated with DOPA liposomes, and cellular uptake is measured using fluorescent or radioactive labels. The lipid's biocompatibility makes it suitable for cell culture applications. DOPA liposomes can be used to study membrane fusion, endocytosis, and intracellular trafficking. The lipid's negatively charged headgroup allows for the study of electrostatic interactions with cell surface proteins. The compound's effects on cell viability and function should be assessed to ensure compatibility with cellular assays. The lipid is typically stored at -20°C and protected from light.
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| Animal Protocol |
In vivo animal experiments with 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt are typically conducted using liposomal formulations for drug delivery studies. DOPA liposomes can be administered intravenously, intraperitoneally, or via other routes to deliver encapsulated drugs. The lipid's biocompatibility and low toxicity make it suitable for in vivo applications. In drug delivery studies, DOPA liposomes are used to improve the pharmacokinetics and biodistribution of therapeutic agents. The lipid's stability in biological fluids allows for sustained drug release. DOPA liposomes can be targeted to specific tissues or cells by incorporating targeting ligands. The compound is not used as a therapeutic agent itself but as a component of drug delivery systems. Dosing and administration protocols depend on the specific drug being delivered and the experimental design.
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| ADME/Pharmacokinetics |
1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt has a molecular weight of approximately 722.95 g/mol and the formula C39H72NaO8P. The lipid is soluble in chloroform and other organic solvents. For long-term storage, the compound is kept at -20°C and protected from light. The lipid forms fluid bilayers at physiological temperatures due to the unsaturated oleic acid chains. The negatively charged phosphate group provides the lipid with anionic properties. The compound is used to prepare liposomes and lipid bilayers for biophysical and biochemical studies. DOPA is a member of the phosphatidic acid family and is used to mimic biological membranes. The compound is intended for research use only and is not approved for clinical use. Its stability and properties make it a valuable tool for membrane research.
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| Toxicity/Toxicokinetics |
The toxicity of 1,2-Dioleoyl-sn-glycero-3-phosphate, sodium salt is low as it is a naturally occurring phospholipid analog used in research applications. The compound is intended for research use only and is not for human use. Standard laboratory safety precautions should be followed when handling the compound, including the use of gloves and eye protection. The lipid is biocompatible and has been used in various drug delivery studies without significant toxicity. In cell culture applications, DOPA liposomes are generally well-tolerated. The compound is not classified as a hazardous substance. Safety data sheets recommend standard handling procedures for research chemicals. The lipid's low toxicity makes it suitable for in vivo applications as a component of drug delivery systems. The compound's effects on cell viability and function should be assessed for specific applications.
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| References |
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| Molecular Formula |
C39H72NAO8P
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| Molecular Weight |
722.9605
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| Exact Mass |
744.468
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| Elemental Analysis |
C, 64.79; H, 10.04; Na, 3.18; O, 17.70; P, 4.28
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| CAS # |
108392-02-5
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| Related CAS # |
61617-08-1 (free acid);108392-02-5 (sodium);
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| PubChem CID |
131865588
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| Appearance |
Colorless to light yellow ointment
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| LogP |
12.502
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
38
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| Heavy Atom Count |
49
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| Complexity |
838
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| Defined Atom Stereocenter Count |
1
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| SMILES |
CCCCCCCC/C=C\CCCCCCCC(=O)OC[C@H](COP(=O)(O)[O-])OC(=O)CCCCCCC/C=C\CCCCCCCC.[Na+]
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| Synonyms |
1,2-dioleoyl-sn-glycero-3-phosphate (sodium salt); DOPA; PA(18:1(9Z)/18:1(9Z));DOPA-NA
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
MEthanol : ~10 mg/mL (~13.83 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3832 mL | 6.9160 mL | 13.8320 mL | |
| 5 mM | 0.2766 mL | 1.3832 mL | 2.7664 mL | |
| 10 mM | 0.1383 mL | 0.6916 mL | 1.3832 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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