| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
(1,1'-Dipyrenyl)dimethyl ether does not have a specific biological target. As a fluorescent probe, its "target" is its local environment within lipid bilayers. The compound partitions into the hydrophobic core of membranes, where its fluorescence properties are influenced by the surrounding lipid environment. Pyrene derivatives form excimers (excited state dimers) at high concentrations or when in close proximity, and the ratio of excimer to monomer fluorescence can be used to measure membrane fluidity and molecular diffusion.
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| ln Vitro |
(1,1'-Dipyrenyl)dimethyl ether exhibits characteristic pyrene fluorescence with monomer emission bands in the 370-400 nm range and excimer emission at approximately 470 nm. The excimer-to-monomer fluorescence ratio (E/M) is sensitive to the local concentration of the probe and the mobility of molecules within the membrane. The compound is used as a probe to study membrane fluidity, lipid diffusion, and lipid-lipid interactions in model membranes and biological membranes. The compound does not exhibit pharmacological activity.
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| ln Vivo |
(1,1'-Dipyrenyl)dimethyl ether does not have in vivo pharmacological activity. It is a fluorescent probe used for research applications, primarily in vitro and ex vivo, to study membrane biophysics. The compound is not administered to animals for therapeutic or pharmacological studies. Its use is limited to research applications in biophysics and membrane biology.
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| Enzyme Assay |
Non-cellular assays for (1,1'-Dipyrenyl)dimethyl ether typically involve its use as a fluorescent probe in liposomes or model membranes. Liposomes are prepared with varying concentrations of the probe, and the fluorescence spectra are recorded using a spectrofluorometer. The excimer-to-monomer (E/M) ratio is calculated from the fluorescence intensities at the excimer and monomer emission maxima. The E/M ratio is correlated with membrane fluidity, lipid packing, or probe concentration.
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| Cell Assay |
In vitro cellular assays for (1,1'-Dipyrenyl)dimethyl ether involve using the probe to study membrane properties in living cells. Cells are incubated with the probe, which partitions into the plasma membrane. The fluorescence of the probe is measured using fluorescence microscopy or spectrofluorometry. Changes in the E/M ratio can indicate changes in membrane fluidity or lipid organization in response to various stimuli or treatments.
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| Animal Protocol |
(1,1'-Dipyrenyl)dimethyl ether is not used in in vivo animal studies as a therapeutic or pharmacological agent. Its application is limited to in vitro and ex vivo research applications for studying membrane biophysics. The compound may be used to study membrane properties in cells isolated from animal models.
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| ADME/Pharmacokinetics |
(1,1'-Dipyrenyl)dimethyl ether is not a drug and does not have pharmacokinetic properties. Its physical and chemical properties are more relevant: it is a pyrene derivative with a molecular weight of 450.57 and a molecular formula of C₃₄H₂₆O. The compound is lipophilic and is soluble in organic solvents. It is typically prepared as a stock solution in organic solvent for addition to aqueous samples.
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| Toxicity/Toxicokinetics |
Specific toxicological data for (1,1'-Dipyrenyl)dimethyl ether are not extensively detailed in standard product information. As a research chemical, it should be handled with standard laboratory precautions, avoiding inhalation, ingestion, and skin contact. The compound is not intended for human use. Pyrene derivatives are generally considered to have low toxicity at the concentrations used for fluorescence studies.
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| References | |
| Additional Infomation |
(1,1'-Dipyrenyl)dimethyl ether is a research-grade fluorescent probe intended for laboratory use only and is not approved for clinical use. Its CAS number is 74833-81-1. The primary applications of (1,1'-Dipyrenyl)dimethyl ether include studying membrane fluidity and lipid bilayer properties, investigating lipid diffusion and lipid-lipid interactions, and as a tool for biophysical research on biological membranes. The compound is a valuable tool for membrane biophysics and cell biology research.
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| Molecular Formula |
C34H22O
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|---|---|
| Molecular Weight |
446.53788
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| Exact Mass |
446.167
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| CAS # |
74833-81-1
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| PubChem CID |
173315
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| Appearance |
Light yellow to yellow solid powder
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| Density |
1.335g/cm3
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| Boiling Point |
671.9ºC at 760 mmHg
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| Melting Point |
169-170ºC
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| Flash Point |
408.8ºC
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| Index of Refraction |
1.89
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| LogP |
9.198
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
35
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| Complexity |
698
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KDWSQWLDGXGQTK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C34H22O/c1-3-21-7-9-25-11-13-27(29-17-15-23(5-1)31(21)33(25)29)19-35-20-28-14-12-26-10-8-22-4-2-6-24-16-18-30(28)34(26)32(22)24/h1-18H,19-20H2
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| Chemical Name |
1-(pyren-1-ylmethoxymethyl)pyrene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2394 mL | 11.1972 mL | 22.3944 mL | |
| 5 mM | 0.4479 mL | 2.2394 mL | 4.4789 mL | |
| 10 mM | 0.2239 mL | 1.1197 mL | 2.2394 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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